Protein-inorganic hybrid flowers with a two-stage accelerated strategy for stimulated activation of CRISPR/Cas12a enhance polynucleotide kinase biosensing

Talanta, Journal Year: 2025, Volume and Issue: 292, P. 127981 - 127981

Published: March 21, 2025

Language: Английский

---

CRISPR/Cas12a-based transition state molecular switch for low-background detection of HPV-16 on a microfluidic platform

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143556 - 143556

Published: April 1, 2025

Language: Английский

---

Nanomaterials functionalized signal-ON/OFF electrogenerated chemiluminescence biosensor for quantization of trypsin based on target-induced cleavage of peptide

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(5)

Published: April 4, 2025

Language: Английский

---

Electrochemiluminescence immunosensor for the sensitive detection of the pancreatic neuroendocrine tumor biomarker chromogranin B based on graphdiyne quantum dots

Analytical Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Construction of the sensitive label-free ECL immunosensor BSA/anti-CgB/GDY QDs@Au NPs/GCE and its application for detection pNET marker chromogranin B.

Language: Английский

---

β-Galactosidase-Mediated, Mn²⁺-Activated CRISPR/Cas12a Cascade Reaction for Immunosorbent Assay of Carbendazim

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

The CRISPR/Cas12a system is an emerging enzymatic tool for the development of enzyme-linked immunosorbent assay (ELISA) methods, owing to its robust signal amplification capability. Currently, most CRISPR/Cas12a-based ELISA approaches rely on strategies that convert target detection into nucleic acid analysis. This report presents a novel cascade reaction transduction and in method, utilizing β-galactosidase (β-gal)-mediated activation system. Carbendazim (CBD), widely used versatile broad-spectrum benzimidazole fungicide, was chosen as model analyte. In absence CBD, streptavidin-labeled β-gal captured by biotinylated secondary antibody immobilized microplate. catalyzes hydrolysis p-aminophenyl β-D-galactopyranoside generate p-aminophenol. compound subsequently facilitates decomposition MnO2 nanosheets, leading generation Mn2+ ions. ions modulate activity system, thus producing high fluorescence solution. presence amount microplate reduced, thereby preventing effective cleavage reporter molecule Cas12a, which results low signal. After systematically optimizing experimental conditions, developed method successfully detected demonstrating sensitivity, selectivity, applicability complex food matrices. comparison traditional acid-activated our approach, integrates β-gal-mediated, Mn2+-activated reactions ELISA, exhibits superior analytical performance, broadening sensitive convenient small-molecule

Language: Английский

---

Photoelectrochemical/fluorescent detection of NAD+ based on gold nanoparticles acted as ethanol dehydrogenase

Talanta, Journal Year: 2025, Volume and Issue: unknown, P. 128274 - 128274

Published: May 1, 2025

Language: Английский

---

Hierarchical ZnVO/g-C₃N₄ Nanoflowers with a Mesoporous Architecture for Photoelectrochemical Detection of Dopamine

ACS Applied Nano Materials, Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Language: Английский

---