Oxidative High Mobility Group Box-1 Accelerates Mitochondrial Transfer from Mesenchymal Stem Cells to Colorectal Cancer Cells Providing Cancer Cell Stemness

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1192 - 1192

Published: Jan. 30, 2025

Mitochondria are important organelles for cell metabolism and tissue survival. Their cell-to-cell transfer is the fate of recipient cells. Recently, bone marrow mesenchymal stem cells (BM-MSCs) have been reported to provide mitochondria cancer rescue mitochondrial dysfunction in However, details mechanism not yet fully elucidated. In this study, we investigated humoral factors inducing (MT) mechanisms. BM-MSCs produced MT colorectal (CRC) damaged by 5-fluorouracil (5-FU), but were suppressed anti-high mobility group box-1 (HMGB1) antibody. treated with oxidized HMGB1 had increased expression MT-associated genes, whereas reduced did not. Inhibition nuclear factor–κB, a downstream factor signaling, significantly decreased gene expression. CRC showed stemness 5-FU sensitivity correlation levels. mouse subcutaneous tumor model CRC, from host BM-MSCs. These results suggest that induces MTs MSCs promotes stemness. Targeting may attenuate CRCs.

Language: Английский

Functional Tumor Boundaries Revealed by Dynamic Electroimpedance Imaging Under Weak Stimuli: Dissipative Structures in Melanoma Area

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

Abstract Real-time imaging and monitoring of tumor boundaries are critical for understanding growth dynamics ensuring precise treatment. All physiological phenomena, specifically communicational, inherently bioelectrical. Our original technology “Skin Electrodynamic Introscopy” (SEI) firstly enabled time-lapse skin spectral electroimpedance landscape (SEL) with an unprecedented combination temporal spatial resolution. SEI the revelation several new phenomena in collective dynamics: emergence dynamic SEL structures melanoma region response to weak short stimuli, such as ischemia non-thermal EMF MF. The main objective this work was identify criteria assessing functional a its peritumor ecosystem. These conceptualized dissipative (DS)—self-organizing patterns that arise nonequilibrium thermodynamic systems, malignant tumors serving striking example. It is shown impedance exceed optical dimensions can vary at different levels intra- intercellular environment. proposed quasi-stationary manifestation entropy/negentropy flow from tumor. boundary be assessed by revealing pre-bifurcation zone measuring increment stimulation resulting activation emitters. In context, invasive front detected appearance antiphase structure border. Thus, previously discovered well number ones, considered first demonstrations DS tissue cellular level. determined primarily increased group sensitivity/state cells their components, rather than individual elements. insight into test-induced behavior allows more nuanced (i.e. local system) metabolism compared traditional reductionist approaches. nature observed most effectively represented fluctuation correlation fields SEL, reflecting thus fundamental concept DS: ‘Order chaos’, which especially relevant biology. particular, demonstrated as: - Transition initially chaotic specific back upon cessation stimulus; Abrupt shift magnitude depth front; Manifestation stroma pre-existing nevus mitochondrial field; Emergence resistance influence systemic post-effect, etc; Moreover, difference images frames made it possible trace process form of: Wave-like around morphological tumor; Expansion initial microclusters boundary; Reverse migration towards negentropy From chaos’ perspective, similarity found between manifestations information (EMF) energy (ischemia) influence. Herewith we also attempted estimate content spatio-temporal version ion balance index (as ratio modulus 2 kHz 1 MHz). support hypothesis, supplement presents analysis two experiments: Papillomatous model exploring viral theory cancer), revealed similar complementary effects reorganization SEL; Plant (plant leaf), demonstrating universal phenomenon structuring. summary, melanoma, particularly context boundaries, reveals insights how progression. discovery represents significant advancement our developed approach highlights potential advanced techniques, it: facilitates real-time biofeedback laboratory clinical settings; need paradigm cancer research; challenge views on exposure induce biological responses level; calls investigating entities shaped interactions environmental factors, thereby opening up opportunities innovative treatment approaches target adaptive behavior.

Language: Английский

Mitochondrial Transplantation in Brain Disorders: Achievements, Methods, and Challenges

Neuroscience & Biobehavioral Reviews, Journal Year: 2024, Volume and Issue: unknown, P. 105971 - 105971

Published: Dec. 1, 2024

Mitochondrial transplantation is a new treatment strategy aimed at repairing cellular damage by introducing healthy mitochondria into injured cells. The approach shows promise in protecting brain function various neurological disorders such as traumatic injury/ischemia, neurodegenerative diseases, cognitive disorders, and cancer. These conditions are often characterized mitochondrial dysfunction, leading to impaired energy production neuronal death. review highlights promising preclinical studies where has been shown restore function, reduce inflammation, improve motor functions several animal models. It also addresses significant challenges that must be overcome before this therapy can clinically applied. Current efforts these challenges, including advancements isolation techniques, cryopreservation methods, finding an appropriate source, potential delivery routes, discussed. Considering the rising incidence of limited effectiveness current treatments, offers comprehensive overview state research critically assesses remaining obstacles. provides valuable insights could steer future potentially lead more effective treatments for disorders.

Language: Английский