RNA-based diagnostic innovations: A new frontier in diabetes diagnosis and management

Diabetes and Vascular Disease Research, Journal Year: 2025, Volume and Issue: 22(2)

Published: March 1, 2025

Background/Objective: Diabetes mellitus (DM) remains a major global health challenge due to its chronic nature and associated complications. Traditional diagnostic approaches, though effective, often lack the sensitivity required for early-stage detection. Recent advancements in molecular biology have identified RNA molecules, particularly non-coding RNAs such as microRNAs (miRNAs), long (lncRNAs), circular (circRNAs), promising biomarkers diabetes. This review aims explore role of RNA-based diagnosis, prognosis, management diabetes, highlighting their potential revolutionize diabetes care. Method: A comprehensive literature was conducted using electronic databases including PubMed, Scopus, Web Science. Articles published up 2024 were screened analyzed extract relevant findings related diagnostics Emphasis placed on studies demonstrating clinical utility, mechanistic insights, translational molecules. Results: Numerous species, miRNAs miR-375, miR-29, lncRNAs like H19 MEG3, exhibit altered expression patterns diabetic patients. These molecules are involved key regulatory pathways glucose metabolism, insulin resistance, β-cell function. Circulating detectable various biofluids, enabling non-invasive approaches. Emerging technologies, sequencing liquid biopsy platforms, enhanced specificity detection, fostering development novel tools personalized therapeutic strategies. Conclusion: hold significant promise advancing early risk stratification, monitoring Despite current challenges standardization validation, integration into routine practice could transform management, paving way precision medicine Further research multi-center trials essential validate these facilitate approval implementation.

Language: Английский

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Phosphorylation of an RNA‐Binding Protein Rck/Me31b by Hippo Is Essential for Adipose Tissue Aging

Aging Cell, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

ABSTRACT The metazoan lifespan is determined in part by a complex signaling network that regulates energy metabolism and stress responses. Key hubs this include insulin/IGF‐1, AMPK, mTOR, sirtuins. Hippo/Mammalian Ste20‐like Kinase1 (MST1) pathway has been reported to maintain Caenorhabditis elegans , but its role not studied higher metazoans. In study, we report overexpression of Hpo, the MST1 homolog Drosophila melanogaster decreased with concomitant changes lipid aging‐associated gene expression, while RNAi Hpo depletion increased lifespan. These effects were mediated primarily Hpo‐induced transcriptional activation RNA‐binding protein maternal expression at 31B (Me31b)/RCK, resulting stabilization mRNA‐encoding lipolytic hormone, Akh. mouse adipocytes, Hpo/Mst1 adipocyte differentiation, phosphorylation proteins such as Rck, decapping MRNA 2 (Dcp2), enhancer Of 3 (Edc3), nucleolin (NCL), glucagon mRNA stability interacting Rck. Decreased Hpo‐overexpressing lines required Me31b, DCP2, which was potentially recovering metabolic genes formation droplets. Taken together, our findings suggest plays conserved longevity regulating adipogenesis fatty acid metabolism.

Language: Английский

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Anti-obesity and immunomodulatory effects of oil and fermented extract dried from Tenebrio molitor larvae on aged obese mice

Animal Cells and Systems, Journal Year: 2024, Volume and Issue: 28(1), P. 340 - 352

Published: July 13, 2024

Preventing disease and maintaining the health of elderly are crucial goals for an aging population, with obesity immune function restoration being paramount importance. Obesity, particularly visceral characterized by excessive fat accumulation around abdominal organs, is linked to chronic conditions such as diabetes, hypertension, cardiovascular diseases, dysfunction. Globally, considered a disease, prompting significant research interest in its treatment. Therefore, it essential explore potential therapeutic preventive strategies address decline brought about aging.

Language: Английский

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The emerging era of multi-disciplinary metabolism research

Molecules and Cells, Journal Year: 2024, Volume and Issue: 47(2), P. 100032 - 100032

Published: Feb. 1, 2024

Language: Английский

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Obesity uncovers presence of inflammatory lung macrophage subsets with adipose tissue transcriptomic signature in influenza virus infection

The Journal of Infectious Diseases, Journal Year: 2024, Volume and Issue: 231(2), P. e317 - e327

Published: Nov. 1, 2024

Obesity is an independent risk factor for increased disease severity during influenza A virus (IAV) infection. White adipose tissue (WAT) inflammation promotes pathogenesis in obesity. Whether obesity modifies lung and WAT immune cells to amplify unknown. We show that establishes a proinflammatory transcriptome augmented IAV infection changes cell milieu Notably, decrease macrophages (ATM) inversely correlates with increase infiltrating obese IAV-infected mice. Further analyses of uncovered macrophage subset shares transcriptomic signature inflammatory ATMs. Importantly, adoptive transfer ATMs from mice into lean infected host infiltration the lungs. These findings suggest that, state, may exacerbate important pathologic responses

Language: Английский

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