bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 20, 2024
Abstract
The
progression
of
the
cell
cycle
is
regulated
by
expression
specific
genes
and
fluctuations
in
cellular
metabolic
states.
Previous
research
has
employed
cycle-based
transcriptomics,
proteomics,
metabolomics
analyses
to
identify
cycle-dependent
changes
at
gene
expression,
protein,
levels.
However,
role
protein
compartmentalization
regulating
function,
coupled
with
evidence
that
enzymes
can
localize
nucleus
influence
chromatin
states,
suggests
nuclear
metabolism
may
play
a
progression.
In
this
study,
we
developed
an
approach
resolve
during
unbiased
systematic
manner.
This
was
achieved
integrating
fluorescent
reporters
mass
spectrometry
imaging.
Our
investigation
focused
on
revealed
phosphatidylinositol
localizes
Moreover,
disruption
affects
distribution
4,5-bisphosphate,
alters
number
morphology
nucleoli,
influences
maintenance
distinct
heterochromatin
states
throughout
cycle.
Finally,
given
established
link
between
methionine
synthesis,
as
well
differential
impact
observed
histone
marks
when
perturbed,
proposed
pools
be
involved
decorate
