Current Opinion in Genetics & Development, Journal Year: 2021, Volume and Issue: 67, P. 142 - 150
Published: Feb. 7, 2021
Language: Английский
Current Opinion in Genetics & Development, Journal Year: 2021, Volume and Issue: 67, P. 142 - 150
Published: Feb. 7, 2021
Language: Английский
Nature Communications, Journal Year: 2018, Volume and Issue: 9(1)
Published: Jan. 9, 2018
Despite an abundance of new studies about topologically associating domains (TADs), the role genetic information in TAD formation is still not fully understood. Here we use our software, HiCExplorer (hicexplorer.readthedocs.io) to annotate >2800 high-resolution (570 bp) boundaries Drosophila melanogaster. We identify eight DNA motifs enriched at boundaries, including a motif bound by M1BP protein, and two boundary motifs. In contrast mammals, CTCF only on small fraction flanking inactive chromatin while most active contain or Beaf-32 proteins. demonstrate that can be accurately predicted using sequences open sites. propose sequence guides genome architecture allocation proteins genome. Finally, present interactive online database access explore spatial organization fly, mouse human genomes, available http://chorogenome.ie-freiburg.mpg.de .
Language: Английский
Citations
820Molecular Cell, Journal Year: 2019, Volume and Issue: 76(2), P. 306 - 319
Published: Sept. 11, 2019
Language: Английский
Citations
193Nature Reviews Genetics, Journal Year: 2018, Volume and Issue: 20(1), P. 39 - 50
Published: Oct. 24, 2018
Language: Английский
Citations
177Signal Transduction and Targeted Therapy, Journal Year: 2023, Volume and Issue: 8(1)
Published: Nov. 10, 2023
Abstract Proper subcellular localization is crucial for the functioning of biomacromolecules, including proteins and RNAs. Nuclear transport a fundamental cellular process that regulates many macromolecules within nuclear or cytoplasmic compartments. In humans, approximately 60 are involved in transport, nucleoporins form membrane-embedded pore complexes, karyopherins cargoes through these Ran system ensure directed rapid transport. Many play additional essential roles mitosis, biomolecular condensation, gene transcription. Dysregulation linked to major human diseases such as cancer, neurodegenerative diseases, viral infections. Selinexor (KPT-330), an inhibitor targeting export factor XPO1 (also known CRM1), was approved 2019 treat two types blood cancers, dozens clinical trials ongoing. This review summarizes three decades research data this field but focuses on structure function individual from recent studies, providing cutting-edge holistic view role health disease. In-depth knowledge rapidly evolving has potential bring new insights into biology, pathogenic mechanisms, therapeutic approaches.
Language: Английский
Citations
71Molecular Cell, Journal Year: 2019, Volume and Issue: 77(1), P. 67 - 81.e7
Published: Nov. 26, 2019
Language: Английский
Citations
90Nature Genetics, Journal Year: 2019, Volume and Issue: 51(12), P. 1723 - 1731
Published: Nov. 29, 2019
Language: Английский
Citations
87Trends in Biochemical Sciences, Journal Year: 2021, Volume and Issue: 46(7), P. 595 - 607
Published: Feb. 7, 2021
Language: Английский
Citations
87Neuron, Journal Year: 2019, Volume and Issue: 102(3), P. 587 - 601.e7
Published: March 12, 2019
Language: Английский
Citations
70Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)
Published: May 25, 2020
Abstract Nucleoporin proteins (Nups) have been proposed to mediate spatial and temporal chromatin organization during gene regulation. Nevertheless, the molecular mechanisms in mammalian cells are not well understood. Here, we report that 153 (NUP153) interacts with architectural proteins, CTCF cohesin, mediates their binding across cis -regulatory elements TAD boundaries mouse embryonic stem (ES) cells. NUP153 depletion results altered cohesin differential expression — specifically at bivalent developmental genes. To investigate mechanism, utilize epidermal growth factor (EGF)-inducible immediate early genes (IEGs). We find controls POL II pausing basal state. Furthermore, efficient IEG transcription relies on NUP153. propose links nuclear pore complex (NPC) architecture allowing poised respond rapidly cues be properly modulated.
Language: Английский
Citations
68eLife, Journal Year: 2017, Volume and Issue: 6
Published: May 24, 2017
The budding yeast Saccharomyces cerevisiae is a long-standing model for the three-dimensional organization of eukaryotic genomes. However, even in this well-studied model, it unclear how homolog pairing diploids or environmental conditions influence overall genome organization. Here, we performed high-throughput chromosome conformation capture on diverged hybrid to obtain first global view diploid yeasts. After controlling Rabl-like orientation using polymer observe significant proximity that increases saturated culture conditions. Surprisingly, localized increase homologous interactions between HAS1-TDA1 alleles specifically under galactose induction and growth. This accompanied by relocalization nuclear periphery requires Nup2, suggesting role pore complexes. Together, these results reveal has dynamic complex 3D
Language: Английский
Citations
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