Molecular Cell,
Journal Year:
2019,
Volume and Issue:
76(4), P. 600 - 616.e6
Published: Nov. 1, 2019
Highlights•R-loops
formed
within
plasmids
promote
antisense
transcription
in
nuclear
extracts•TSS
of
lncRNA
and
eRNA
are
often
near
R-loop
structures
sensitive
to
RNase
H1•Preinitiation
complexes
associated
with
synthesis
dependent•Many
mammalian
derive
from
promoter
activitySummaryWidespread
long
noncoding
RNA
(lncRNA)
overlap
many
protein-coding
genes
mammals
emanate
gene
promoter,
enhancer,
termination
regions.
However,
their
origin
biological
purpose
remain
unclear.
We
show
that
these
can
be
generated
by
R-loops
form
when
nascent
transcript
invades
the
DNA
duplex
behind
elongating
polymerase
II
(Pol
II).
Biochemically,
act
as
intrinsic
Pol
promoters
induce
de
novo
synthesis.
Furthermore,
removal
across
human
genome
H1
overexpression
causes
selective
reduction
transcription.
Consequently,
we
predict
facilitate
proximal
lncRNA.
Not
only
widely
damage
repair,
but
now
they
have
capacity
may
aided
evolution
regulation.Graphical
abstract
Genes & Development,
Journal Year:
2019,
Volume and Issue:
33(15-16), P. 960 - 982
Published: May 23, 2019
Precise
spatio-temporal
control
of
gene
activity
is
essential
for
organismal
development,
growth,
and
survival
in
a
changing
environment.
Decisive
steps
regulation
involve
the
pausing
RNA
polymerase
II
(Pol
II)
early
elongation,
controlled
release
paused
into
productive
synthesis.
Here
we
describe
factors
that
enable
events
trigger
Pol
gene.
We
also
discuss
open
questions
field
concerning
stability
II,
nucleosomes
as
obstacles
to
potential
roles
defining
precision
dynamics
expression.
