Embryonic Temperature Programs Phenotype in Reptiles DOI Creative Commons
Sunil Kumar Singh, Debojyoti Das, Turk Rhen

et al.

Frontiers in Physiology, Journal Year: 2020, Volume and Issue: 11

Published: Jan. 31, 2020

Reptiles are critically affected by temperature throughout their lifespan, but especially so during early development. Temperature induced changes in phenotype a specific example of broader phenomenon called phenotypic plasticity which single individual is able to develop different phenotypes when exposed environments. With climate change occurring at an unprecedented rate, it important study effects on reptiles. For example, the potential impact global warming pronounced species with temperature-dependent sex determination (TSD) because has direct effect key (sex) and demographic (population ratios) trait. TSD also serve as models for studying development other traits that display continuous variation. directly influences metabolic developmental rate embryos can have permanent last beyond embryonic period. instance, incubation programs post-hatching hormone production growth physiology, profoundly influence fitness. Here, we review current knowledge First, examine biophysical processes, concept thermal performance curves, process acclimation. After discussing these reversible effects, focus bulk programming embryogenesis (i.e., effects). We oviparous eggs susceptible ambient temperature. then discuss recent work probing role epigenetic mechanisms mediating phenotype. Based temperature, return Finally, highlight areas future research, including identification sensors assessment genetic variation thermosensitivity.

Language: Английский

The molecular principles of gene regulation by Polycomb repressive complexes DOI
Neil P. Blackledge, Robert J. Klose

Nature Reviews Molecular Cell Biology, Journal Year: 2021, Volume and Issue: 22(12), P. 815 - 833

Published: Aug. 16, 2021

Language: Английский

Citations

338
Histone H2AK119 Mono-Ubiquitination Is Essential for Polycomb-Mediated Transcriptional Repression DOI Creative Commons
Simone Tamburri,

Elisa Lavarone,

Daniel Fernández-Pérez

et al.

Molecular Cell, Journal Year: 2019, Volume and Issue: 77(4), P. 840 - 856.e5

Published: Dec. 26, 2019

Polycomb group proteins (PcGs) maintain transcriptional repression to preserve cellular identity in two distinct repressive complexes, PRC1 and PRC2, that modify histones by depositing H2AK119ub1 H3K27me3, respectively. PRC2 exist different variants show a complex regulatory cross-talk. However, the contribution plays mediating PcG functions remains largely controversial. Using fully catalytic inactive RING1B mutant, we demonstrated deposition is essential PcG-target gene embryonic stem cells (ESCs). Loss of induced rapid displacement activity loss H3K27me3 deposition. This preferentially affected PRC2.2 variant with respect PRC2.1, destabilizing canonical activity. Finally, found forms can sense deposition, which contributes their stabilization specifically at sites where this modification highly enriched. Overall, our data place as central hub mounts machineries cell identity.

Language: Английский

Citations

302
The roles of Polycomb repressive complexes in mammalian development and cancer DOI
Andrea Piunti, Ali Shilatifard

Nature Reviews Molecular Cell Biology, Journal Year: 2021, Volume and Issue: 22(5), P. 326 - 345

Published: March 15, 2021

Language: Английский

Citations

298
PRC1 Catalytic Activity Is Central to Polycomb System Function DOI Creative Commons
Neil P. Blackledge, Nadezda A. Fursova, Jessica R. Kelley

et al.

Molecular Cell, Journal Year: 2019, Volume and Issue: 77(4), P. 857 - 874.e9

Published: Dec. 27, 2019

The Polycomb repressive system is an essential chromatin-based regulator of gene expression. Despite being extensively studied, how the selects its target genes poorly understood, and whether histone-modifying activities are required for transcriptional repression remains controversial. Here, we directly test requirement PRC1 catalytic activity in function. To achieve this, develop a conditional mutation embryonic stem cells that completely removes activity. Using this system, demonstrate catalysis by drives chromatin domain formation long-range interactions. Furthermore, show variant complexes with DNA-binding occupy sites independently activity, providing putative mechanism site selection. Finally, discover Polycomb-mediated requires Together these discoveries provide compelling evidence central to function regulation.

Language: Английский

Citations

224
Polycomb Gene Silencing Mechanisms: PRC2 Chromatin Targeting, H3K27me3 'Readout', and Phase Separation-Based Compaction DOI
Yiran Guo, Shuai Zhao, Gang Greg Wang

et al.

Trends in Genetics, Journal Year: 2021, Volume and Issue: 37(6), P. 547 - 565

Published: Jan. 22, 2021

Language: Английский

Citations

121
Regulation, functions and transmission of bivalent chromatin during mammalian development DOI
Trisha A. Macrae, Julie Fothergill-Robinson, Miguel Ramalho‐Santos

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2022, Volume and Issue: 24(1), P. 6 - 26

Published: Aug. 26, 2022

Language: Английский

Citations

119
H2AK119ub1 guides maternal inheritance and zygotic deposition of H3K27me3 in mouse embryos DOI
Hailiang Mei, Chisayo Kozuka,

Ryoya Hayashi

et al.

Nature Genetics, Journal Year: 2021, Volume and Issue: 53(4), P. 539 - 550

Published: April 1, 2021

Language: Английский

Citations

107
Mechanisms of Polycomb group protein function in cancer DOI Creative Commons
Victoria Parreno, Anne‐Marie Martinez, Giacomo Cavalli

et al.

Cell Research, Journal Year: 2022, Volume and Issue: 32(3), P. 231 - 253

Published: Jan. 19, 2022

Cancer arises from a multitude of disorders resulting in loss differentiation and stem cell-like phenotype characterized by uncontrolled growth. Polycomb Group (PcG) proteins are members multiprotein complexes that highly conserved throughout evolution. Historically, they have been described as essential for maintaining epigenetic cellular memory locking homeotic genes transcriptionally repressed state. What was initially thought to be function restricted few target genes, subsequently turned out much broader relevance, since the main role PcG is ensure dynamically choregraphed spatio-temporal regulation their numerous during development. Their ability modify chromatin landscapes refine expression master controlling major switches decisions under physiological conditions often misregulated tumors. Surprisingly, functional implication initiation progression cancer may either dependent on complexes, or specific subunit acts independently other members. In this review, we describe how play pleiotropic altering broad spectrum biological processes such proliferation-differentiation balance, metabolism immune response, all which crucial tumor progression. We also illustrate interfering with functions can provide powerful strategy counter

Language: Английский

Citations

104
Context-specific Polycomb mechanisms in development DOI
Jongmin Kim, Robert E. Kingston

Nature Reviews Genetics, Journal Year: 2022, Volume and Issue: 23(11), P. 680 - 695

Published: June 9, 2022

Language: Английский

Citations

72
The apparent loss of PRC2 chromatin occupancy as an artifact of RNA depletion DOI Creative Commons
Evan Healy, Qi Zhang,

Emma H. Gail

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(3), P. 113858 - 113858

Published: Feb. 27, 2024

RNA has been implicated in the recruitment of chromatin modifiers, and previous studies have provided evidence favor against this idea. RNase treatment is commonly used to study RNA-mediated regulation but limitations approach remain unclear. A during immunoprecipitation (ChIP) reduces occupancy H3K27me3 methyltransferase Polycomb repressive complex 2 (PRC2). This led suggestions an "RNA bridge" between PRC2 chromatin. Here, we show that ChIP causes apparent loss all facultative heterochromatin, including both genome-wide. We track observation a gain DNA from non-targeted chromatin, sequenced at expense which signals. Our results emphasize substantial using for mapping RNA-dependent invalidate conclusions were previously established based on assay.

Language: Английский

Citations

19