Journal of Proteome Research,
Journal Year:
2023,
Volume and Issue:
22(12), P. 3773 - 3779
Published: Nov. 1, 2023
Accurate
measurements
of
the
molecular
composition
single
cells
will
be
necessary
for
understanding
relationship
between
gene
expression
and
function
in
diverse
cell
types.
One
most
important
phenotypes
that
differs
is
their
size,
which
was
recently
shown
to
an
determinant
proteome
populations
similarly
sized
cells.
We,
therefore,
sought
test
if
effects
size
on
protein
concentrations
were
also
evident
single-cell
proteomics
data.
Using
relative
a
set
reference
proteins
estimate
cell's
DNA-to-cell
volume
ratio,
we
found
differences
explain
significant
amount
cell-to-cell
variance
two
published
data
sets.
Proceedings of the National Academy of Sciences,
Journal Year:
2024,
Volume and Issue:
121(33)
Published: Aug. 6, 2024
The
mammalian
mitochondrial
genome
(mtDNA)
is
multicopy
and
its
copy
number
(mtCN)
varies
widely
across
tissues,
in
development
disease.
Here,
we
systematically
catalog
this
variation
by
assaying
mtCN
52
human
tissues
952
donors
(10,499
samples
from
the
Genotype-Tissue
Expression
project)
20
murine
using
qPCR,
capturing
50-
200-fold
variation,
respectively.
We
also
estimate
per
cell
173
lines
Cancer
Cell
Line
Encyclopedia
whole-genome
sequencing
data
observe
>50-fold
variation.
then
leverage
vast
amount
of
genomics
available
for
these
repositories
to
credential
our
resource
uncover
mtDNA-related
biology.
Using
already
existing
proteomics
data,
show
that
can
be
predicted
TFAM,
histone,
ribosome
protein
abundance.
integrate
estimates
with
CRISPR
gene
dependency
measurements
find
high
are
resistant
loss
GPX4,
a
glutathione
phospholipid
hydroperoxidase.
Our
captures
should
broadly
useful
research
community.
Molecular Biology of the Cell,
Journal Year:
2022,
Volume and Issue:
33(9)
Published: Aug. 1, 2022
Cells
adopt
a
size
that
is
optimal
for
their
function,
and
pushing
them
beyond
this
limit
can
cause
cell
aging
death
by
senescence
or
reduce
proliferative
potential.
However,
increasing
genome
copy
number
(ploidy),
cells
increase
dramatically
homeostatically
maintain
physiological
properties
such
as
biosynthesis
rate.
Recent
studies
investigating
the
relationship
between
rates
of
metabolism
under
normal,
polyploid,
pathological
conditions
are
revealing
new
insights
into
how
attain
best
function
fitness
tuning
processes
including
transcription,
translation,
mitochondrial
respiration.
A
frontier
to
connect
single-cell
scaling
relationships
with
tissue
whole-organism
physiology,
which
promises
reveal
molecular
evolutionary
principles
underlying
astonishing
diversity
observed
across
tree
life.
Journal of Proteome Research,
Journal Year:
2023,
Volume and Issue:
22(12), P. 3773 - 3779
Published: Nov. 1, 2023
Accurate
measurements
of
the
molecular
composition
single
cells
will
be
necessary
for
understanding
relationship
between
gene
expression
and
function
in
diverse
cell
types.
One
most
important
phenotypes
that
differs
is
their
size,
which
was
recently
shown
to
an
determinant
proteome
populations
similarly
sized
cells.
We,
therefore,
sought
test
if
effects
size
on
protein
concentrations
were
also
evident
single-cell
proteomics
data.
Using
relative
a
set
reference
proteins
estimate
cell's
DNA-to-cell
volume
ratio,
we
found
differences
explain
significant
amount
cell-to-cell
variance
two
published
data
sets.
