Telomere protein arrays stall DNA loop extrusion by condensin DOI Creative Commons
Brian T. Analikwu, Alice Deshayes, Jaco van der Torre

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 29, 2023

Abstract DNA loop extrusion by SMC proteins is a key process underlying chromosomal organization. It unknown how extruders interact with telomeres where chromosome ends are covered dense array of tens neighboring DNA-binding proteins. Using complementary in vivo and vitro single-molecule approaches, we study the interaction between loop-extruding condensin arrays Rap1, double-stranded-DNA-binding telomeric protein Saccharomyces cerevisiae . We show that linear Rap1 can completely halt extrusion, blocking efficiency depends on length gap size In cells, found to act as contact insulators accumulate at their borders, direct implications for resolution dicentric chromosomes produced telomere fusions. Our findings DNA-bound efficiently proteins, which may impact wide range cellular processes from functions transcription repair.

Language: Английский

Genome control by SMC complexes DOI
Claire Hoencamp, Benjamin D. Rowland

Nature Reviews Molecular Cell Biology, Journal Year: 2023, Volume and Issue: 24(9), P. 633 - 650

Published: May 25, 2023

Language: Английский

Citations

65
A host of armor: Prokaryotic immune strategies against mobile genetic elements DOI Creative Commons
David Mayo-Muñoz, Rafael Pinilla‐Redondo, Nils Birkholz

et al.

Cell Reports, Journal Year: 2023, Volume and Issue: 42(7), P. 112672 - 112672

Published: June 21, 2023

Prokaryotic adaptation is strongly influenced by the horizontal acquisition of beneficial traits via mobile genetic elements (MGEs), such as viruses/bacteriophages and plasmids. However, MGEs can also impose a fitness cost due to their often parasitic nature differing evolutionary trajectories. In response, prokaryotes have evolved diverse immune mechanisms against MGEs. Recently, our understanding abundance diversity prokaryotic systems has greatly expanded. These defense degrade invading material, inhibit genome replication, or trigger abortive infection, leading population protection. this review, we highlight these strategies, focusing on most recent discoveries. The study defenses not only sheds light microbial evolution but uncovers novel enzymatic activities with promising biotechnological applications.

Language: Английский

Citations

65
How do interactions between mobile genetic elements affect horizontal gene transfer? DOI Creative Commons
Tanya Horne, Victoria T Orr, James P. J. Hall

et al.

Current Opinion in Microbiology, Journal Year: 2023, Volume and Issue: 73, P. 102282 - 102282

Published: Feb. 28, 2023

Horizontal gene transfer is central to bacterial adaptation and facilitated by mobile genetic elements (MGEs). Increasingly, MGEs are being studied as agents with their own interests adaptations, the interactions have one another recognised having a powerful effect on flow of traits between microbes. Collaborations conflicts nuanced can both promote inhibit acquisition new material, shaping maintenance newly acquired genes dissemination important adaptive through microbiomes. We review recent studies that shed light this dynamic oftentimes interlaced interplay, highlighting importance genome defence systems in mediating MGE-MGE conflicts, outlining consequences for evolutionary change, resonate from molecular microbiome ecosystem levels.

Language: Английский

Citations

52
Prokaryotic Gabija complex senses and executes nucleotide depletion and DNA cleavage for antiviral defense DOI Creative Commons
Rui Cheng, Fengtao Huang, Xueling Lu

et al.

Cell Host & Microbe, Journal Year: 2023, Volume and Issue: 31(8), P. 1331 - 1344.e5

Published: July 21, 2023

Language: Английский

Citations

31
Multiple enzymatic activities of a Sir2-HerA system cooperate for anti-phage defense DOI Creative Commons
Dongmei Tang, Yijun Chen, Hao Chen

et al.

Molecular Cell, Journal Year: 2023, Volume and Issue: 83(24), P. 4600 - 4613.e6

Published: Dec. 1, 2023

Language: Английский

Citations

25
Structural basis for plasmid restriction by SMC JET nuclease DOI Creative Commons
Florian Roisné-Hamelin, Hon Wing Liu, Michael J. Taschner

et al.

Molecular Cell, Journal Year: 2024, Volume and Issue: 84(5), P. 883 - 896.e7

Published: Feb. 2, 2024

DNA loop-extruding SMC complexes play crucial roles in chromosome folding and immunity. Prokaryotic Wadjet (JET) limit the spread of plasmids through cleavage, yet mechanisms for plasmid recognition are unresolved. We show that artificial circularization renders linear susceptible to JET nuclease cleavage. Unlike free DNA, cleaves immobilized at a specific site, plasmid-anchoring point, showing anchor hinders extrusion but not Structures plasmid-bound JetABC reveal two presumably stalled motor units drastically rearranged from resting state, together entrapping U-shaped segment, which is further converted kinked V-shaped cleavage substrate by JetD binding. Our findings uncover mechanical bending residual unextruded as molecular signature non-self elimination. moreover elucidate key elements loop extrusion, including direction structure DNA-holding state.

Language: Английский

Citations

15
Plasmid targeting and destruction by the DdmDE bacterial defence system DOI
Jack P. K. Bravo, Delisa Ramos, Rodrigo Fregoso Ocampo

et al.

Nature, Journal Year: 2024, Volume and Issue: 630(8018), P. 961 - 967

Published: May 13, 2024

Language: Английский

Citations

14
Multi-layered genome defences in bacteria DOI Creative Commons
Aleksei Agapov, Kate S. Baker,

Paritosh Bedekar

et al.

Current Opinion in Microbiology, Journal Year: 2024, Volume and Issue: 78, P. 102436 - 102436

Published: Feb. 17, 2024

Bacteria have evolved a variety of defence mechanisms to protect against mobile genetic elements, including restriction-modification systems and CRISPR–Cas. In recent years, dozens previously unknown (DSs) been discovered. Notably, diverse DSs often coexist within the same genome, some co-occur at frequencies significantly higher than would be expected by chance, implying potential synergistic interactions. Recent studies provided evidence that enhance or complement one another. Here, we review interactions between mechanistic, regulatory, ecological evolutionary levels.

Language: Английский

Citations

12
Molecular mechanism of plasmid elimination by the DdmDE defense system DOI
Luuk Loeff, David W. Adams, Christelle Chanez

et al.

Science, Journal Year: 2024, Volume and Issue: 385(6705), P. 188 - 194

Published: June 13, 2024

Seventh-pandemic

Language: Английский

Citations

12
The MksG nuclease is the executing part of the bacterial plasmid defense system MksBEFG DOI Creative Commons
Manuela Weiß,

Giacomo Giacomelli,

Mathilde Ben-Assaya

et al.

Nucleic Acids Research, Journal Year: 2023, Volume and Issue: 51(7), P. 3288 - 3306

Published: March 7, 2023

Abstract Cells are continuously facing the risk of taking up foreign DNA that can compromise genomic integrity. Therefore, bacteria in a constant arms race with mobile genetic elements such as phages, transposons and plasmids. They have developed several active strategies against invading molecules be seen bacterial ‘innate immune system’. Here, we investigated molecular arrangement Corynebacterium glutamicum MksBEFG complex, which is homologous to MukBEF condensin system. We show here MksG nuclease degrades plasmid DNA. The crystal structure revealed dimeric assembly through its C-terminal domain TOPRIM topoisomerase II family enzymes contains corresponding ion binding site essential for cleavage topoisomerases. MksBEF subunits exhibit an ATPase cycle vitro reason this reaction cycle, combination activity provided by MksG, allows processive degradation Super-resolution localization microscopy Mks system spatially regulated via polar scaffold protein DivIVA. Introduction plasmids results increase bound indicating activation vivo.

Language: Английский

Citations

20