DNA Replication and Polymer Chain Duplication Reshape the Genome in Space and Time DOI Creative Commons
Dario D’Asaro, Maxime M. C. Tortora, Cédric Vaillant

et al.

Physical Review X, Journal Year: 2024, Volume and Issue: 14(4)

Published: Oct. 22, 2024

In eukaryotes, DNA replication constitutes a complex process whereby multiple origins are stochastically fired, and from which the machinery proceeds along chromosomes to achieve faithful synthesis of two identical copies genome during S phase cell cycle. Experimental evidence shows functional correlation between dynamics spatial organization inside nuclei, suggesting that replicating may impact chromosome folding. However, theoretical mechanistic bases such hypothesis remain elusive. To address question, we propose quantitative, minimal framework integrates polymer chain by accounting explicitly for progression resulting formation sister chromatids. By systematically characterizing 3D structural consequences replication, possible interactions active machineries, show transient loops potentially across temporal scales, level individual global chain. Comparison with available microscopy conformation capture data in yeast suggests replication-dependent loop extrusion be acting , shape as loose bottle brushes phase. Lastly, explore postreplication relative chromatids demonstrate emergence catenations intertwined structures, regulated density fired origins. Published American Physical Society 2024

Language: Английский

DNA Replication and Polymer Chain Duplication Reshape the Genome in Space and Time DOI Creative Commons
Dario D’Asaro, Maxime M. C. Tortora, Cédric Vaillant

et al.

Physical Review X, Journal Year: 2024, Volume and Issue: 14(4)

Published: Oct. 22, 2024

In eukaryotes, DNA replication constitutes a complex process whereby multiple origins are stochastically fired, and from which the machinery proceeds along chromosomes to achieve faithful synthesis of two identical copies genome during S phase cell cycle. Experimental evidence shows functional correlation between dynamics spatial organization inside nuclei, suggesting that replicating may impact chromosome folding. However, theoretical mechanistic bases such hypothesis remain elusive. To address question, we propose quantitative, minimal framework integrates polymer chain by accounting explicitly for progression resulting formation sister chromatids. By systematically characterizing 3D structural consequences replication, possible interactions active machineries, show transient loops potentially across temporal scales, level individual global chain. Comparison with available microscopy conformation capture data in yeast suggests replication-dependent loop extrusion be acting , shape as loose bottle brushes phase. Lastly, explore postreplication relative chromatids demonstrate emergence catenations intertwined structures, regulated density fired origins. Published American Physical Society 2024

Language: Английский

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