Levan-type fructooligosaccharides synthesis by novel levansucrase-inulosucrase fusion enzyme

Biochemical Engineering Journal, Journal Year: 2022, Volume and Issue: 185, P. 108524 - 108524

Published: June 23, 2022

Language: Английский

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Unraveling the role of flexible coil near calcium binding site of levansucrase on thermostability and product profile via proline substitution and molecular dynamics simulations

Proteins Structure Function and Bioinformatics, Journal Year: 2023, Volume and Issue: 92(2), P. 170 - 178

Published: Sept. 27, 2023

Abstract Due to its bioactivity and versatile applications, levan has appeared as a promising biomaterial. Levansucrase is responsible for the conversion of sucrose into levan. With goal enhancing production, strategy stability levansucrase being intensively studied. To make proteins more stable under high temperatures, proline, most rigid residue, can be introduced previously flexible regions. Herein, G249, D250, N251, H252 on coil close calcium binding site Bacillus licheniformis were replaced with proline. Mutations at G249P greatly enhance both enzyme's thermodynamic kinetic stability, while those H252P improve solely stability. GPC analysis revealed that synthesize levan, but generate primarily oligosaccharides. Molecular dynamics simulations (MD) MM/GBSA mutation increased not only levansucrase, also affinity toward fructan.

Language: Английский

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Molecular advances in microbial α-galactosidases: challenges and prospects

World Journal of Microbiology and Biotechnology, Journal Year: 2022, Volume and Issue: 38(9)

Published: July 1, 2022

Language: Английский

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A theoretical study on binding and stabilization of galactose and novel galactose analogues to the human α-galactosidase A variant causing Fabry disease

Biophysical Chemistry, Journal Year: 2022, Volume and Issue: 292, P. 106915 - 106915

Published: Oct. 27, 2022

Language: Английский

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