Precise Intracellular Uptake and Endosomal Release of Diverse Functional mRNA Payloads via Glutathione-Responsive Nanogels DOI Creative Commons
Rupali Dabas,

Naveenan Navaratnam,

Haruki Iino

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 30, P. 101425 - 101425

Published: Dec. 29, 2024

We present a novel, highly customizable glutathione-responsive nanogel (NG) platform for efficient mRNA delivery with precise payload release control. Optimization of various cationic monomers, including newly synthesized polyarginine, polyhistidine, and acrylated guanidine allowed fine-tuning NG properties binding. By incorporating poly(ethylene) glycol-based disulphide crosslinker, we achieved glutathione-triggered release, enabling targeted intracellular delivery. Our NGs demonstrated superior encapsulation (up to 89.3 %) loading (10.7 efficiencies, controlled kinetics at glutathione concentrations. outperformed commercial transfection reagents across multiple cell lines, traditionally difficult-to-transfect lines. demonstrate the platform's versatility by successfully delivering GFP mRNA, Mango II RNA aptamers, functionally relevant β2-AMPK mRNA. Furthermore, used TIRF microscopy measure exact copy number within NGs. Notably, mechanistic cellular uptake studies revealed that disulphide-containing exhibit enhanced endosomal escape, potentially due interactions surface thiols. This work represents tuneable, efficient, biocompatible relevance gene therapy vaccine development.

Language: Английский

Shaping the future: latest developments in 3D printing stimuli-responsive soft polymers DOI
Govind Vashishtha, Sumika Chauhan, Nitin Yadav

et al.

The International Journal of Advanced Manufacturing Technology, Journal Year: 2025, Volume and Issue: 136(10), P. 4215 - 4237

Published: Feb. 1, 2025

Language: Английский

Citations

1
Nanogels: Recent Advances in Synthesis and Biomedical Applications DOI Open Access

Pasquale Mastella,

Biagio Todaro, Stefano Luin

et al.

Published: July 10, 2024

in the context of advanced nanomaterials research, nanogels have recently gained considerable attention for their versatility and promising biomedical applications. To date, a significant number been developed to meet growing demands various fields research. Summarising preparation methods, physicochemical biological properties recent applications may be useful help exploring new directions development. This article presents comprehensive overview latest nanogel synthesis methodologies, highlighting advances formulation with different types hydrophilic or amphiphilic polymers. It also underlines drug delivery imaging, short section dedicated biosafety considerations these innovative nanomaterials. In conclusion, this summarises innovations numerous applications, potential field.

Language: Английский

Citations

5
Flavonoid-Based Nanogels: A Comprehensive Overview DOI Creative Commons
Sergio Liga, Cristina Paul

Gels, Journal Year: 2025, Volume and Issue: 11(4), P. 267 - 267

Published: April 4, 2025

The growing field of nanotechnology has recently given much attention to nanogels, which are versatile formulas and have promising biomedical applications. Nanogels or nanohydrogels undergone significant development in various fields industrial research meet increasing demands, such as pharmaceuticals, cosmetics, food, genetic engineering. that contain flavonoids, secondary metabolites found plants, starting become distinctive reveal their unique characteristics. objective the article is provide a comprehensive overview recent articles on flavonoid-based emphasizing general aspects regarding nanogel formulation structural characterization, well advancements made field. In conclusion, this outlines up-to-date developments synthesis, formulation, therapeutic applications important role nanotechnology.

Language: Английский

Citations

0
Targeting cell signaling pathway ALKBH5/Beclin1/ULK1 in lung cancer by 5-flurouracil- loaded P (AAm/SA) nanogel in rats DOI
Ahmad S. Kodous,

Eman Salah Eldin,

Hebatallah E. Mohamed

et al.

APOPTOSIS, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Intelligent responsive nanogels: New Horizons in cancer therapy DOI
Mazlina Musa, Xinxin Sun, Jianbin Shi

et al.

International Journal of Pharmaceutics, Journal Year: 2024, Volume and Issue: 669, P. 125050 - 125050

Published: Dec. 5, 2024

Language: Английский

Citations

3
Hybrid Systems of Gels and Nanoparticles for Cancer Therapy: Advances in Multifunctional Therapeutic Platforms DOI Creative Commons
Kibeom Kim

Gels, Journal Year: 2025, Volume and Issue: 11(3), P. 170 - 170

Published: Feb. 26, 2025

Cancer is a global health concern. Various therapeutic approaches, including chemotherapy, photodynamic therapy, and immunotherapy, have been developed for cancer treatment. Silica nanoparticles, quantum dots, metal–organic framework (MOF)-based nanomedicines gained interest in therapy because of their selective accumulation tumors via the enhanced permeability retention (EPR) effect. However, bare nanoparticles face challenges poor biocompatibility, low stability, limited drug-loading capacity, rapid clearance by reticuloendothelial system (RES). Gels with unique three-dimensional network structures formed through various interactions such as covalent hydrogen bonds are emerging promising materials addressing these challenges. Gel hybridization enhances facilitates controlled drug release, confers cancer-targeting abilities to nanoparticles. This review discusses gel–nanoparticle hybrid systems treatment past five years analyzes roles gels systems.

Language: Английский

Citations

0
Nanogels: Recent Advances in Synthesis and Biomedical Applications DOI Creative Commons

Pasquale Mastella,

Biagio Todaro, Stefano Luin

et al.

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(15), P. 1300 - 1300

Published: Aug. 1, 2024

In the context of advanced nanomaterials research, nanogels (NGs) have recently gained broad attention for their versatility and promising biomedical applications. To date, a significant number NGs been developed to meet growing demands in various fields research. Summarizing preparation methods, physicochemical biological properties, recent applications may be useful help explore new directions development. This article presents comprehensive overview latest NG synthesis methodologies, highlighting advances formulation with different types hydrophilic or amphiphilic polymers. It also underlines drug delivery imaging, short section dedicated biosafety considerations these innovative nanomaterials. conclusion, this summarizes innovations numerous applications, considerable potential field.

Language: Английский

Citations

2
Dual Temperature and pH Sensitive Poly(ethyl methacrylate‐co‐acrylamide) Nanogels Have Potential for Controlled Release of Doxorubicin DOI

Orhun Hakkoymaz,

Hidayet Mazı

ChemistrySelect, Journal Year: 2024, Volume and Issue: 9(37)

Published: Sept. 30, 2024

Abstract In this study, the synthesis and characterization of poly(ethyl methacrylate‐ co ‐acrylamide) (i.e., poly(EMA‐ ‐AAm)) nanogels loading release studies doxorubicin hydrochloride were carried out. The was out using emulsion polymerization technique at 60 °C. Swelling experiments showed that sensitive to temperature pH medium. Zeta sizer analysis results synthesized had an average size 23.17 nm PDI value 0.270. into nanogels, drug capacity (DLC) nanogel determined as 32% efficiency (DLE) 77%. Release conducted = 5.4 7.4 37 °C percentage 17% even in 32 h 7.4, 82% 5 5.4. different temperatures revealed °C, 85% 39 89% 41 These ‐AAm) can be used targeted controlled increase effects while reducing side effects.

Language: Английский

Citations

0
Precise Intracellular Uptake and Endosomal Release of Diverse Functional mRNA Payloads via Glutathione-Responsive Nanogels DOI Creative Commons
Rupali Dabas,

Naveenan Navaratnam,

Haruki Iino

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 30, P. 101425 - 101425

Published: Dec. 29, 2024

We present a novel, highly customizable glutathione-responsive nanogel (NG) platform for efficient mRNA delivery with precise payload release control. Optimization of various cationic monomers, including newly synthesized polyarginine, polyhistidine, and acrylated guanidine allowed fine-tuning NG properties binding. By incorporating poly(ethylene) glycol-based disulphide crosslinker, we achieved glutathione-triggered release, enabling targeted intracellular delivery. Our NGs demonstrated superior encapsulation (up to 89.3 %) loading (10.7 efficiencies, controlled kinetics at glutathione concentrations. outperformed commercial transfection reagents across multiple cell lines, traditionally difficult-to-transfect lines. demonstrate the platform's versatility by successfully delivering GFP mRNA, Mango II RNA aptamers, functionally relevant β2-AMPK mRNA. Furthermore, used TIRF microscopy measure exact copy number within NGs. Notably, mechanistic cellular uptake studies revealed that disulphide-containing exhibit enhanced endosomal escape, potentially due interactions surface thiols. This work represents tuneable, efficient, biocompatible relevance gene therapy vaccine development.

Language: Английский

Citations

0