Biotechnology and Bioprocess Engineering, Journal Year: 2025, Volume and Issue: unknown
Published: March 4, 2025
Language: Английский
Bioactive Materials, Journal Year: 2024, Volume and Issue: 35, P. 346 - 361
Published: Feb. 14, 2024
The impaired differentiation ability of resident cells and disordered immune microenvironment in periodontitis pose a huge challenge for bone regeneration. Herein, we construct piezoelectric hydrogel to rescue the osteogenic capability rebuild regenerative through bioenergetic activation. Under local mechanical stress, generated piezopotential that initiates inflammatory periodontal ligament stem (PDLSCs) via modulating energy metabolism promoting adenosine triphosphate (ATP) synthesis. Moreover, it also reshapes an anti-inflammatory pro-regenerative niche switching M1 macrophages M2 phenotype. synergy tilapia gelatin stimulation enhances situ regeneration defects rats. These findings pave new pathway treating other immune-related stimulation-enabled modulation immunomodulation.
Language: Английский
Citations
34
Advanced Materials, Journal Year: 2024, Volume and Issue: 36(15)
Published: Jan. 31, 2024
Abstract Cellular energetics plays an important role in tissue regeneration, and the enhanced metabolic activity of delivered stem cells can accelerate repair regeneration. However, conventional hydrogels with limited network cell adaptability restrict cell–cell interactions activities. In this work, it is shown that a cell‐adaptable hydrogel high dynamics enhances glucose uptake fatty acid β ‐oxidation encapsulated human mesenchymal (hMSCs) compared low dynamics. It further hMSCs dynamic exhibit increased tricarboxylic (TCA) cycle activity, oxidative phosphorylation (OXPHOS), adenosine triphosphate (ATP) biosynthesis via E‐cadherin‐ AMP‐activated protein kinase (AMPK)‐dependent mechanism. The vivo evaluation showed delivery MSCs by situ bone regeneration animal model. believed findings provide critical insights into impact cell–biomaterial on cellular underlying mechanisms.
Language: Английский
Citations
14
Advanced Science, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 17, 2025
Extracellular matrix (ECM) derived from mesenchymal stem cells regulates antioxidant properties and bone metabolism by providing a favorable extracellular microenvironment. However, its functional role molecular mechanism in mitochondrial function regulation aged regeneration remain insufficiently elucidated. This proteomic analysis has revealed greater abundance of proteins supporting the young ECM (Y-ECM) secreted marrow-derived (BMMSCs) compared to (A-ECM). Further studies demonstrate that Y-ECM significantly rejuvenates energy adult BMMSCs (A-BMMSCs) through promotion respiratory functions amelioration oxidative stress. A-BMMSCs cultured on exhibited enhanced multi-lineage differentiation potentials vitro ectopic formation vivo. Mechanistically, silencing silent information regulator type 3 (SIRT3) gene abolished protective impact A-BMMSCs. Notably, novel composite biomaterial combining hyaluronic acid methacrylate hydrogel microspheres with is developed, which yielded substantial improvements healing defects an rat model. Collectively, these findings underscore pivotal maintaining redox homeostasis present promising therapeutic strategy for repair defects.
Language: Английский
Citations
1
Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 2, 2025
With the global population aging, awareness of oral health is rising. Periodontitis, a widespread bacterial infectious disease, gaining attention. Current novel biomaterials address key clinical issues like infection, gum inflammation, tooth loosening, and loss, focusing on antibacterial, anti-inflammatory, tissue regeneration properties. However, strategies that integrate advantages these to achieve synergistic therapeutic effects by clearing biofilms, inhibiting inflammation activation, restoring periodontal soft hard functions remain very limited. Recent studies highlight link between periodontitis systemic diseases, underscoring complexity disease. There an urgent need find comprehensive treatment plans requirements. Whether integrating new enhance existing treatments or developing approaches replace traditional therapies, efforts will drive advancements in treatment. Therefore, this review compares with treatments. It highlights design concepts mechanisms functional materials, their properties, discusses importance strategies. This aims provide guidance for emerging research promote development precise efficient
Language: Английский
Citations
1
Molecular Metabolism, Journal Year: 2022, Volume and Issue: 66, P. 101617 - 101617
Published: Oct. 18, 2022
Language: Английский
Citations
30
International Journal of Oral Science, Journal Year: 2023, Volume and Issue: 15(1)
Published: Dec. 1, 2023
Abstract Many tissues and organ systems have intrinsic regeneration capabilities that are largely driven maintained by tissue-resident stem cell populations. In recent years, growing evidence has demonstrated cellular metabolic homeostasis plays a central role in mediating fate, tissue regeneration, homeostasis. Thus, thorough understanding of the mechanisms regulate cells may contribute to our knowledge on how is provide novel insights for disease management. this review, we summarize known relationship between regulation molecular pathways cells. We also discuss potential targets therapy describe current limitations future directions development these therapeutic targets.
Language: Английский
Citations
18
Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14
Published: Aug. 14, 2023
Osteoporosis (OP) is characterized by a decrease in osteoblasts and an increase adipocytes the bone marrow compartment, alongside abnormal bone/fat differentiation, which ultimately results imbalanced homeostasis. Bone mesenchymal stem cells (BMSCs) can differentiate into to maintain Several studies have shown that lncRNAs are competitive endogenous RNAs form lncRNA–miRNA network targeting miRNA for regulation of differentiation BMSCs; this mechanism closely related corresponding treatment OP important development novel OP-targeted therapies. However, reviewing current literature, it became clear there limited summaries discussing effects on osteogenic/adipogenic BMSCs. Therefore, article provides review literature explore impact BMSCs, with aim providing new theoretical basis OP.
Language: Английский
Citations
17
Advanced Science, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 11, 2024
Abstract Coordinating the immune response and bioenergy metabolism in bone defect environments is essential for promoting regeneration. Mitochondria are important organelles that control internal balance metabolism. Repairing dysfunctional mitochondria has been proposed as a therapeutic approach disease intervention. Here, an engineered hierarchical hydrogel with responsiveness can adapt to regeneration environment mediate targeted transfer between cells. The continuous supply of by macrophages restore mitochondrial marrow mesenchymal stem cells (BMSC). Fundamentally solving problem insufficient energy support BMSCs caused local inflammation during repair This discovery provides new strategy repair, which research value practical application prospects treatment various diseases dysfunction.
Language: Английский
Citations
8
ACS Biomaterials Science & Engineering, Journal Year: 2022, Volume and Issue: 8(12), P. 5060 - 5093
Published: Nov. 23, 2022
This review focuses on the advancements in additive manufacturing techniques that are utilized for fabricating bioceramic scaffolds and their characterizations leading to bone tissue regeneration. Bioscaffolds made by mimicking human structure, material composition, properties. Calcium phosphate apatite materials most commonly used scaffold as they closely resemble live inorganic composition. The functionally graded fabricated utilizing right choice of 3D printing method combinations achieve requirement bioscaffold. To tailor physical, mechanical, biological properties scaffold, certain reinforced, doped, or coated incorporate functionality. biomechanical loading conditions involve flexion, torsion, tension exerted implanted discussed. finite element analysis (FEA) technique is investigate mechanical property before fabrication. helps reducing actual number samples testing. FEA simulated results experimental result compared. also highlights some challenges associated while processing such shrinkage, instability, cytotoxicity, printability. In end, new evolved engineering applications compiled
Language: Английский
Citations
27
Combinatorial Chemistry & High Throughput Screening, Journal Year: 2024, Volume and Issue: 27(11), P. 1611 - 1628
Published: Jan. 12, 2024
Osteoporosis (OP) is an age-related skeletal disease. Kaempferol can regulate bone mesenchymal stem cells (BMSCs) osteogenesis to improve OP, but its mechanism related disulfidptosis, a newly discovered cell death mechanism, remains unclear.
Language: Английский
Citations
6