Advanced domestication: harnessing the precision of gene editing in crop breeding DOI
Wendy J. Lyzenga, Curtis Pozniak, Sateesh Kagale

et al.

Plant Biotechnology Journal, Journal Year: 2021, Volume and Issue: 19(4), P. 660 - 670

Published: March 4, 2021

Summary Human population growth has increased the demand for food crops, animal feed, biofuel and biomaterials, all while climate change is impacting environmental conditions. There an urgent need to develop crop varieties which tolerate adverse conditions requiring fewer inputs. Plant breeding critical global security and, it benefited from modern technologies, remains constrained by a lack of valuable genetic diversity, linkage drag, effective way combine multiple favourable alleles complex traits. CRISPR/Cas technology transformed genome editing across biological systems promises transform agriculture with its high precision, ease design, multiplexing ability low cost. We discuss integration CRISPR/Cas‐based gene into advance domestication refine inbred various applications environments. highlight use fix desirable allelic variants, generate novel alleles, break deleterious linkages, support pre‐breeding introgression loci elite lines.

Language: Английский

Enhancing the productivity and resilience of rice (Oryza sativa) under environmental stress conditions using clustered regularly interspaced short palindromic repeats (CRISPR) technology DOI
Aamir Riaz, Muhammad Uzair, Ali Raza

et al.

Functional Plant Biology, Journal Year: 2025, Volume and Issue: 52(1)

Published: Jan. 2, 2025

Rice (Oryza sativa ) is a crucial staple crop worldwide, providing nutrition to more than half of the global population. Nonetheless, sustainability grain production increasingly jeopardized by both biotic and abiotic stressors exacerbated climate change, which increases crop's rvulnerability pests diseases. Genome-editing clustered regularly interspaced short palindromic repeats CRISPR-associated Protein 9 (CRISPR-Cas9) presents potential solution for enhancing rice productivity resilience under climatic stress. This technology can alter plant's genetic components without introduction foreign DNA or genes. It has become one most extensively used approaches discovering new gene functions creating novel varieties that exhibit higher tolerance stresses, herbicide resistance, improved yield production. study examines numerous CRISPR-Cas9-based genome-editing techniques knockout, knock-in, multiplexing simultaneous disruption multiple genes, base-editing, prime-editing. review elucidates application technologies enhance directly targeting yield-related genes indirectly modulating stress-responsive We highlight need integrate advancements with conventional advanced agricultural methods create are resilient thereby safeguarding food security promoting amid concerns.

Language: Английский

Citations

2
Molecular Basis of Disease Resistance and Perspectives on Breeding Strategies for Resistance Improvement in Crops DOI Creative Commons
Yiwen Deng, Yuese Ning, Dong‐Lei Yang

et al.

Molecular Plant, Journal Year: 2020, Volume and Issue: 13(10), P. 1402 - 1419

Published: Sept. 29, 2020

Language: Английский

Citations

106
High-efficiency and multiplex adenine base editing in plants using new TadA variants DOI Creative Commons

Daqi Yan,

Bin Ren, Lang Liu

et al.

Molecular Plant, Journal Year: 2021, Volume and Issue: 14(5), P. 722 - 731

Published: Feb. 23, 2021

Language: Английский

Citations

98
Genome editing for plant research and crop improvement DOI Creative Commons
Xiangqiang Zhan, Yuming Lu, Jian‐Kang Zhu

et al.

Journal of Integrative Plant Biology, Journal Year: 2020, Volume and Issue: 63(1), P. 3 - 33

Published: Dec. 25, 2020

The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a profound impact on plant biology, and crop improvement. In this review, we summarize the state-of-the-art development CRISPR technologies their applications in plants, from initial introduction random small indel (insertion or deletion) mutations at target genomic loci to precision editing such as base editing, prime gene targeting. We describe advances use class 2, types II, V, VI systems for disruption well precise sequence alterations, transcription, epigenome control.

Language: Английский

Citations

97
Improved plant cytosine base editors with high editing activity, purity, and specificity DOI Creative Commons

Qiurong Ren,

Simon Sretenovic, Guanqing Liu

et al.

Plant Biotechnology Journal, Journal Year: 2021, Volume and Issue: 19(10), P. 2052 - 2068

Published: May 27, 2021

Summary Cytosine base editors (CBEs) are great additions to the expanding genome editing toolbox. To improve C‐to‐T in plants, we first compared seven cytidine deaminases BE3‐like configuration rice. We found A3A/Y130F‐CBE_V01 resulted highest efficiency both rice and Arabidopsis . Furthermore, demonstrated this A3A/Y130F deaminase could be used iSpyMacCas9‐mediated at A‐rich PAMs. showcase its applications, applied for multiplexed generate microRNA‐resistant mRNA transcripts as well pre‐mature stop codons multiple seed trait genes. In addition, harnessed efficient artificial evolution of novel ALS EPSPS alleles which conferred herbicide resistance further editing, CBE_V02, CBE_V03 CBE_V04 systems were developed tested protoplasts. The have improved activity purity with focal recruitment more uracil DNA glycosylase inhibitors (UGIs) by engineered single guide RNA 2.0 scaffold. Finally, whole‐genome sequencing (WGS) compare six CBE_V01 four genome‐wide off‐target effects Different levels deaminase‐dependent sgRNA‐independent indeed revealed WGS among edited lines these CBE systems. also investigated sgRNA‐dependent different CBEs This comprehensive study 21 systems, benchmarked PmCDA1‐CBE_V04 A3A/Y130F‐CBE_V04 next‐generation plant high efficiency, purity, specificity.

Language: Английский

Citations

92
SpRY greatly expands the genome editing scope in rice with highly flexible PAM recognition DOI Creative Commons
Ziyan Xu, Yongjie Kuang, Bin Ren

et al.

Genome biology, Journal Year: 2021, Volume and Issue: 22(1)

Published: Jan. 4, 2021

Abstract Background Plant genome engineering mediated by various CRISPR-based tools requires specific protospacer adjacent motifs (PAMs), such as the well-performed NGG, NG, and NNG, to initiate target recognition, which notably restricts editable range of plant genome. Results In this study, we thoroughly investigate nuclease activity PAM preference two structurally engineered SpCas9 variants, SpG SpRY, in transgenic rice. Our study shows that favors NGD PAMs, albeit less efficiently than previously described SpCas9-NG, SpRY achieves efficient editing across a wide genomic loci, exhibiting well NAN PAMs. Furthermore, SpRY-fused cytidine deaminase hAID*Δ adenosine TadA8e are generated, respectively. These constructs induce C-to-T A-to-G conversions genes toward non-canonical including non-G Remarkably, high-frequency self-editing events (indels DNA fragments deletion) integrated T-DNA result observed, whereas nickase-mediated base editor is quite low rice lines. Conclusions The broad compatibility greatly expands targeting scope engineering.

Language: Английский

Citations

90
CRISPR-Mediated Engineering across the Central Dogma in Plant Biology for Basic Research and Crop Improvement DOI Creative Commons
Dibyajyoti Pramanik, Rahul Mahadev Shelake, Mi Jung Kim

et al.

Molecular Plant, Journal Year: 2020, Volume and Issue: 14(1), P. 127 - 150

Published: Nov. 3, 2020

The central dogma (CD) of molecular biology is the transfer genetic information from DNA to RNA protein. Major CD processes governing flow include cell cycle, replication, chromosome packaging, epigenetic changes, transcription, posttranscriptional alterations, translation, and posttranslational modifications. are tightly regulated in plants maintain integrity throughout life cycle pass materials next generation. Engineering various involved gene regulation will accelerate crop improvement feed growing world population. CRISPR technology enables programmable editing alter DNA, RNA, or protein, which would have been impossible past. Here, an overview recent advancements tool development CRISPR-based modulations that expedite basic applied plant research provided. Furthermore, applications major thriving areas research, such as discovery (allele mining cryptic activation), introgression (de novo domestication haploid induction), application desired traits beneficial farmers consumers (biotic/abiotic stress-resilient crops, factories, delayed senescence), described. Finally, global regulatory policies, challenges, prospects for CRISPR-mediated discussed.

Language: Английский

Citations

89
A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises DOI Creative Commons
Adnan Rasheed, Rafaqat A. Gill, Muhammad Umair Hassan

et al.

Current Issues in Molecular Biology, Journal Year: 2021, Volume and Issue: 43(3), P. 1950 - 1976

Published: Nov. 11, 2021

Genome editing (GE) has revolutionized the biological sciences by creating a novel approach for manipulating genomes of living organisms. Many tools have been developed in recent years to enable complex genomes. Therefore, reliable and rapid increasing yield tolerance various environmental stresses is necessary sustain agricultural crop production global food security. This critical review elaborates GE used improvement. These include mega-nucleases (MNs), such as zinc-finger nucleases (ZFNs), transcriptional activator-like effector (TALENs), clustered regularly interspaced short palindromic repeats (CRISPR). Specifically, this addresses latest advancements role CRISPR/Cas9 genome manipulation major improvement, including quality development biotic stress- abiotic stress-tolerant crops. Implementation technique will lead non-transgene crops with preferred characteristics that can result enhanced capacity under stresses. The be combined current potential breeding methods (e.g., speed omics-assisted breeding) enhance productivity ensure We also discussed challenges limitations CRISPR/Cas9. information useful plant breeders researchers thorough investigation use boost targeting gene interest.

Language: Английский

Citations

85
CRISPR screens in plants: approaches, guidelines, and future prospects DOI Open Access
Christophe Gaillochet, Ward Develtere, Thomas B. Jacobs

et al.

The Plant Cell, Journal Year: 2021, Volume and Issue: 33(4), P. 794 - 813

Published: April 1, 2021

Abstract Clustered regularly interspaced short palindromic repeat (CRISPR)-associated systems have revolutionized genome engineering by facilitating a wide range of targeted DNA perturbations. These resulted in the development powerful new screens to test gene functions at genomic scale. While there is tremendous potential map and interrogate regulatory networks unprecedented speed scale using CRISPR screens, their implementation plants remains its infancy. Here we discuss general concepts, tools, workflows for establishing analyze handful recent reports describing use this strategy generate mutant knockout collections or diversify sequences. In addition, provide insight into how design given current challenges limitations examine multiple options. Finally, unique multiplexing capabilities investigate redundant highly duplicated plant genomes. Combinatorial routinely higher-order facilitate characterization networks. By integrating approach with numerous profiles that been generated over past two decades, offers opportunities genomes deeper resolution will lead great advances functional synthetic biology.

Language: Английский

Citations

80
Identification of herbicide resistance OsACC1 mutations via in planta prime-editing-library screening in rice DOI

Rongfang Xu,

Xiaoshuang Liu, Juan Li

et al.

Nature Plants, Journal Year: 2021, Volume and Issue: 7(7), P. 888 - 892

Published: June 10, 2021

Language: Английский

Citations

78