Yeast,
Journal Year:
2024,
Volume and Issue:
41(4), P. 148 - 157
Published: Jan. 18, 2024
Abstract
N
6
‐methyladenosine
(m6A)
is
a
highly
abundant
and
evolutionarily
conserved
messenger
RNA
(mRNA)
modification.
This
modification
installed
on
RRACH
motifs
mRNAs
by
hetero‐multimeric
holoenzyme
known
as
m6A
methyltransferase
complex
(MTC).
The
mark
then
recognised
group
of
proteins
the
YTH
domain
family
which
guide
mRNA
for
subsequent
downstream
processes
that
determine
its
fate.
In
yeast,
thousands
during
early
meiosis
MTC
m6A‐modified
are
read
domain‐containing
protein
Mrb1/Pho92.
this
review,
we
aim
to
delve
into
recent
advances
in
our
understanding
regulation
roles
yeast
meiosis.
We
will
discuss
potential
functions
translation
decay,
unravelling
their
significance
regulating
gene
expression.
propose
serves
an
exceptional
model
organism
study
fundamental
molecular
mechanisms
related
function
mRNAs.
insights
gained
from
research
not
only
expand
knowledge
modifications
but
also
offer
valuable
broader
landscape
eukaryotic
posttranscriptional
Frontiers in Plant Science,
Journal Year:
2024,
Volume and Issue:
15
Published: July 16, 2024
The
epitranscriptomic
mark
N
6-methyladenosine
(m6A)
is
the
most
common
type
of
messenger
RNA
(mRNA)
post-transcriptional
modification
in
eukaryotes.
With
discovery
demethylase
FTO
(FAT
MASS
AND
OBESITY-ASSOCIATED
PROTEIN)
Homo
Sapiens,
this
has
been
proven
to
be
dynamically
reversible.
technological
advances,
research
on
m6A
plants
also
rapidly
developed.
widely
distributed
plants,
which
usually
enriched
near
stop
codons
and
3'-UTRs,
conserved
sequences.
related
proteins
mainly
consist
three
components:
methyltransferases
(writers),
demethylases
(erasers),
reading
(readers).
regulates
growth
development
by
modulating
metabolic
processes
playing
an
important
role
their
responses
environmental
signals.
In
review,
we
briefly
outline
detection
techniques;
comparatively
analyze
distribution
characteristics
plants;
summarize
methyltransferases,
demethylases,
binding
m6A;
elaborate
how
functions
plant
growth,
development,
response
signals;
provide
a
summary
outlook
plants.
BMC Plant Biology,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: Feb. 4, 2025
m6A
RNA
modifications
are
the
most
prevalent
internal
in
eukaryotic
mRNAs
and
crucial
for
plant
growth
development,
as
well
responses
to
biotic
or
abiotic
stresses.
The
modification
is
catalyzed
by
writers,
removed
erasers,
decoded
various
m6A-binding
proteins,
which
readers.
Brassica
napus
a
major
oilseed
crop.
dynamic
regulation
of
readers
offers
potential
targets
improving
quality
this
In
study,
we
identified
92
m6A-regulatory
genes
B.
napus,
including
13
29
50
A
phylogenetic
analysis
revealed
that
they
could
be
further
divided
into
four,
three,
two
clades,
respectively.
distribution
protein
motifs
gene
structures
among
members
same
clade
exhibited
notable
similarity.
During
course
evolution,
whole
genome
duplication
(WGD)
segmental
were
primary
drivers
expansion
m6A-related
families.
subjected
rigorous
purification
selection.
Additionally,
several
sites
under
positive
selection
proteins.
RNA-seq
quantitative
real-time
PCR
(qRT-PCR)
expression
analyses
Bnam6As
exhibit
tissue-specific
patterns,
their
patterns
response
2000
bp
sequence
upstream
contained
number
cis-acting
elements
regulate
environmental
response.
Furthermore,
interaction
network
interactions
with
proteins
significant
functional
importance.
identification
modifiers
rape
molecular
evolution
profiling
have
functions
mechanisms
m6A,
thus
establishing
foundation
validation
breeding.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 25, 2025
Abstract
Synchronised
developmental
timing
is
important
for
ensuring
crop
uniformity
and
high
yields.
However,
climate
change
leading
to
crops
being
grown
at
different
latitudes,
varying
their
exposures
photoperiods
over
seasons
impacting
timings.
We
investigated
whether
the
response
of
circadian
rhythms
in
seedlings
changes
photoperiod
would
enable
us
predict
synchronization
flowering.
Indeed,
we
show
that
same
Quantitative
Trait
Loci
(QTLs)
are
associated
traits
during
bolting,
using
first
recombinant
inbred
lines
(RILs)
between
African
European
Arabidopsis
lineages,
spanning
diverse
latitudes.
Two
QTLs
contain
K-Homology
Domain
RNA
binding
proteins
(KH17,
KH29)
with
splicing
variants
known
flowering
genes,
MADS
AFFECTING
FLOWERING2
3
(MAF2,
MAF3),
including
generating
chimeric
transcripts,
a
potential
mechanism
accelerated
proteome
evolution.
Natural
KH17,
its
prion-like
domain,
de-coupling
mean
time,
enabling
greater
adaptation
population-level
heterogeneity
Our
results
suggest
could
be
used
screen
agriculturally
relevant
mature
plants,
efficient
breeding
climate-resilient
crops.
