Innovative Biosystems and Bioengineering, Journal Year: 2023, Volume and Issue: 7(3), P. 55 - 64

Published: Oct. 29, 2023

Background. Excessive melanin accumulation in the skin can lead to various diseases and cosmetic issues. While tyrosinase inhibitors are commonly used reduce pigment biosynthesis, many of them associated with significant side effects. When multiple drugs combination, it result synergism, additive effects, or antagonism. Combining is considered a promising approach minimize effects enhance therapeutic efficacy. Objective. This study aims investigate combined use determine nature their interaction, whether it's synergistic additive. Methods. We utilized isolated from Agaricus bisporus mushrooms. Enzyme inhibition by test compounds was assessed measuring activity using tyrosine (30 min 0.05 M Na-phosphate buffer solution, pH 6.5, 25 °C). To explore joint inhibition, compound solutions were mixed pairs at concentrations. The interaction quantified combination index isobolograms. Results. effect action agents on activity, we examined standard enzyme (kojic acid, arbutin, phenylthiourea) our discovered compound, 3-(2-hydroxyphenylamino)-1,3-dihydro-indol-2-one. Calculations isobolograms for all studied combinations revealed cases. Simultaneous kojic acid arbutin 3-(2-hydroxyphenylamino)-1,3-dihydro-indol-2-one demonstrated effect. However, mixture phenylthiourea indole derivative an Conclusions. usage displayed both two simultaneously presents opportunities development more effective cost-efficient treatments hyperpigmentation reducing concentration each inhibitor.

Language: Английский