In
this
study,
a
comprehensive
investigation
was
undertaken
to
elucidate
simple
triazole
compound,
5-phenyl-1-(p-tolyl)-1H-1,2,3-triazole
(PPTT),
its
interactions
with
high-abundant
proteins
and
identification
of
low-abundant
by
serum
proteomics.
Employing
combination
spectroscopic
techniques
computational
chemistry,
the
between
PPTT
three
high-abundance
blood
globular
proteins,
namely
human
albumin
(HSA),
immunoglobulin
G
(HIgG),
hemoglobin
(BHb),
were
explored,
thereby
ascertaining
their
binding
constants
thermodynamic
parameters
at
molecular
level.
Subsequently,
based
on
differential
proteomics,
utilizing
two-dimensional
gel
electrophoresis
(2-DE)
in
conjunction
matrix-assisted
laser
desorption
time-of-flight
mass
spectrometry
(MALDI-TOF-MS),
research
team
isolated
identified
differentially
expressed
low-abundance
samples
following
exposure
PPTT.
The
results
showed
that
there
twenty
highly
from
intervened
One
apolipoprotein
A-1
(ApoA-1)
protein,
selected
as
possible
target
explore
mechanism
action
intervention
related
signaling
pathways
involved
Hep
G2
cells.
These
findings
offer
scientifically
sound
guidance
for
further
in-depth
exploration,
development,
application
1,2,3-triazole
compound.
