Metabolomics combined with network pharmacology reveals the effects of ripening stages and edible parts on bioactive ingredients of Luohan Guo (Siraitia grosvenorii)

Food Research International, Journal Year: 2025, Volume and Issue: unknown, P. 115896 - 115896

Published: Jan. 1, 2025

Language: Английский

---

Impact of ultrasound-assisted intermittent hydration during pumpkin seed germination on the structure, nutritional, bioactive, physical and techno-functional properties of flours

LWT, Journal Year: 2025, Volume and Issue: unknown, P. 117654 - 117654

Published: March 1, 2025

Language: Английский

---

Quercetin Exhibits Broad‐Spectrum Antibiofilm and Antiquorum Sensing Activities Against Gram‐Negative Bacteria: In Vitro and In Silico Investigation Targeting Antimicrobial Therapy

Canadian Journal of Infectious Diseases and Medical Microbiology, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Quercetin (QC), a flavonoid abundant in fruits and vegetables, has garnered attention for its potential therapeutic properties. In this study, we investigated the antibiofilm antiquorum sensing (QS) activities of QC against Gram‐negative bacteria both vitro silico. The findings study demonstrate MIC values 125 μg/mL Chromobacterium violaceum , 250 Pseudomonas aeruginosa 500 Serratia marcescens indicating antibacterial abilities. QS‐mediated production violacein prodigiosin was significantly inhibited dose‐dependent manner at sub‐MIC concentrations. Additionally, reduction virulence factors P. including pyocyanin, pyoverdine, rhamnolipid, noted with QC. Biofilm formation decreased by 66.40%, 59.28%, 63.70% highest C. S. respectively. Furthermore, swimming motility exopolysaccharide (EPS) were also reduced presence molecular docking dynamics simulations elucidate binding interactions between key targets (LasI, LasR, PilY1, LasA, PilT, CviR, CviR′, PqsR, RhlR, PigG) involved biofilm QS pathways. Our results indicated that anti‐QS may be attributed to ability interfere critical signaling molecules regulatory proteins. Overall, highlights as promising natural compound combating biofilm‐associated infections caused bacteria. multifaceted antimicrobial mechanisms underscore agent treatment biofilm‐related infections, providing way further exploration, development QC‐based strategies therapy.

Language: Английский

---

Anti-cancer effects of Plumbago zeylanica L. against human triple-negative breast cancer: Insights from network pharmacology and in-vitro experimental validation

South African Journal of Botany, Journal Year: 2025, Volume and Issue: 180, P. 795 - 810

Published: April 8, 2025

Language: Английский

---

Comprehensive identification of GASA genes in sunflower and expression profiling in response to drought

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Oct. 14, 2024

Drought stress poses a critical threat to global crop yields and sustainable agriculture. The GASA genes are recognized for their pivotal role in tolerance plant growth, but little is known about how they function sunflowers. investigation aimed identify elucidate the of HaGASA conferring sunflowers with drought tolerance. Twenty-seven different gene family members were found this study that inconsistently located across eleven sunflower chromosomes. Phylogeny analysis revealed divided into five subgroups by comparing those from Arabidopsis, peanut, soybean, within each subgroup displaying similar conserved motifs structures. In-silico evaluation cis-regulatory elements indicated existence specific associated stress-responsiveness being most abundant, followed hormone, light, growth-responsive elements. Transcriptomic data NCBI database was utilized assess expression profile varieties under conditions. ten genotypes stress, 14 differentially expressed genes, implying active plant's response. organs HaGASA2, HaGASA11, HaGASA17, HaGASA19, HaGASA21 HaGASA26 displayed maximum stem. Our findings implicate mediating growth maintenance adaptation abiotic particularly drought. findings, taken together, provided basic understanding structure potential functions setting framework further functional investigations roles mitigation improvement strategies.

Language: Английский

---

Investigation of Egfr Inhibition Potential of Some Natural Compounds from Ganoderma Amboinense Using Dft, Molecular Docking, Molecular Dynamics Simulations, and Binding Free Energy Calculations

Published: Jan. 1, 2024

Language: Английский

---