Identification biomarkers and therapeutic targets of disulfidptosis-related in rheumatoid arthritis via bioinformatics, molecular dynamics simulation, and experimental validation

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 13, 2025

The relationship between disulfidptosis and rheumatoid arthritis (RA) remains unclear. We aimed to identified biomarkers disulfidptosis-related in RA revealed potential targeted drugs. Two microarray datasets (GSE93272, GSE45291) related were downloaded from the Gene Expression Omnibus (GEO) database. Disulfidptosis-related genes(DRGs) extracted FerrDb GSE93272 was used identify DRGs, GSE45291 verify results. Multivariate Cox regression analysis candidate disulfidptosis-associated hub genes. differentiated values of DRGs determined by receiver operator characteristic (ROC) monofactor judge their quality as biomarkers. RT-qPCR validate expression Additionally, we analyzed connection genes filtration immune cells RA. made predictions about miRNAs, TFs possible drugs that regulate Subsequently, molecular docking carried out predict combination with targets. Finally, dynamics simulation conducted further findings. Oxoacyl-ACP Synthase Mitochondrial(OXSM) a biomarker high diagnostic value, an model based on OXSM for single gene constructed. showed accuracy distinguishing healthy controls (AUC = 0.802) validated external datasets, showing excellent power 0.982). Twelve against recognized comparative toxicogenomics database (CTD). Molecular results ICG 001 had highest binding affinity OXSM, simulations confirmed stability this complexes. Furthermore, CIBERSORT significant correlation cell infiltration regulatory network TFs-gene-miRNAs comprising 8 miRNAs 34 identified. significantly increased peripheral blood patients compared controls, consistent bioinformatics analysis. These studies suggest may be therapeutic target diagnosing RA, drug findings provide new avenues effective diagnosis treatment

Language: Английский

Germination response of Erigeron sumatrensis (Retz.) seeds to drying and freezing

Agrociencia Uruguay, Journal Year: 2025, Volume and Issue: 29, P. e1496 - e1496

Published: March 24, 2025

Erigeron species form a complex of weed that have evolved herbicide resistance in Uruguay. Genetic studies using seeds from different biotypes are frequently conducted to understand how this evolution occurred. However, these extremely small, and there is limited information available on their long-term conservation germplasm banks. In context, the aim study was characterize drying strategies for seed storage, comparing prolonged low-temperature (20 days at 20 °C, 15% RH) with shorter, higher-temperature (10 40 °C or 7 60 °C), assess germination responses subsequent freezing (–20 30 days). The results showed not affected by any treatments. Moreover, batches increased germination, particularly more recently harvested lots (2020 2021), especially when combined °C. These findings indicate E. sumatrensis can be classified as orthodox banks standard protocols feasible.

Language: Английский