The Interaction Mechanism of Ziprasidone With Calf Thymus DNA by Multiple Spectrums And Molecular Docking

Published: Jan. 1, 2025

Language: Английский

Label-Free Liquid Crystal Biosensor Based on Split Aptamers for Detecting Serum Myoglobin

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Myoglobin (Mb) is a critical cytoplasmic heme protein and one of the earliest detectable biomarkers following acute myocardial infarction (MI). The rapid precise detection serum Mb holds significant clinical value. This study capitalizes on advantages split aptamer (Apt) sequence, which short effectively minimizes false-positive signals caused by secondary structure probe. It constructs "sandwich"-structured liquid crystal biosensor with an "Apt-target molecule-Apt" configuration for label-free Mb. Apt fragments were immobilized surface glass substrate as capture probes (CP) using triethoxybutanal silane (TEA) linker molecules. When present, other fragment, signal probe (SP), specifically binds reassociates CP, restoring original stem-loop Apt, thereby inducing change in alignment 5CB leads to variations brightness color optical polarizing microscope image, facilitating quantitative method capable detecting concentrations ranging from 0.05 130 ng/mL, minimum limit 0.045 ng/mL. sandwich biosensing offers several advantages, including detection, operational simplicity, high specificity. demonstrates potential biomarkers.

Language: Английский

The binding investigation of ziprasidone with calf thymus DNA by multiple spectrums and molecular docking

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: unknown, P. 126217 - 126217

Published: April 1, 2025

Language: Английский