Label‐Free Detection of π‐Stacking Interactions During Tryptophan Self‐Assembling Into Amyloid‐Like Structures Using Surface‐Enhanced Raman Scattering

Journal of Raman Spectroscopy, Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

ABSTRACT The formation of amyloid fibrils by different proteins and peptides is a well‐studied topic. In the last decade, it has been reported that metabolites can also self‐assemble into amyloid‐like fibrils. aggregation single amino acids such as phenylalanine, tyrosine, tryptophan linked to tyrosinemia type II, hypertryptophanemia, Hartnup diseases. It challenge monitor intermolecular interactions involved in supramolecular self‐assembling time‐dependent manner. Here, surface‐enhanced Raman scattering (SERS) technique was used directly probe changes molecular during structures, particularly initial stages. We showed this simple label‐free nanoplasmonic‐based methodology be examine at level aggregates function time. Specifically, shown sensitive provide insights hydrogen bonding, π‐stacking interactions, hydrophobicity tryptophan. Thus, work open new possibilities for applications SERS describe more detail mechanisms other assembled which may valuable understanding several related

Language: Английский

Simplified Biochemical Analysis Using p-Block Metals and Their Compounds with Silver─Surface Enhancement from the Point of View of Electronic Structure

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: May 7, 2025

Language: Английский