Xenopus laevis as an infection model for human pathogenic bacteria DOI Creative Commons

Ayano Kuriu,

Kazuya Ishikawa, K. Tsuchiya

et al.

Infection and Immunity, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Animal infection models are essential for understanding bacterial pathogenicity and corresponding host immune responses. In this study, we investigated whether juvenile Xenopus laevis could be used as an model human pathogenic bacteria. frogs succumbed to intraperitoneal injection containing the bacteria Staphylococcus aureus, Pseudomonas aeruginosa, Listeria monocytogenes. contrast, non-pathogenic Bacillus subtilis Escherichia coli did not induce mortality in frogs. The administration of appropriate antibiotics suppressed caused by S. aureus P. aeruginosa. Strains lacking agr locus, cvfA (rny) gene, or hemolysin genes LIPI-1-deleted mutant L. monocytogenes, which attenuate virulence within mammals, exhibited reduced compared with their respective wild-type counterparts. Bacterial distribution analysis revealed that persisted blood, liver, heart, muscles until death. These results suggested induces sepsis-like symptoms frogs, supporting use a valuable animal evaluating antimicrobial efficacy identifying various

Language: Английский

Optimization of the drug-induced cholestasis index based on advanced modeling for predicting liver toxicity DOI
Annika Drees, Vahid Nassiri, Andrés Tabernilla

et al.

Toxicology, Journal Year: 2025, Volume and Issue: unknown, P. 154119 - 154119

Published: March 1, 2025

Language: Английский

Citations

0
New Approach Methods (NAMs) for Genotoxicity Assessment of Nano- and Advanced Materials; Advantages and Challenges DOI

Arno C. Gutleb,

Sivakumar Murugadoss,

Maciej Stępnik

et al.

Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Journal Year: 2025, Volume and Issue: unknown, P. 503867 - 503867

Published: March 1, 2025

Language: Английский

Citations

0
Xenopus laevis as an infection model for human pathogenic bacteria DOI Creative Commons

Ayano Kuriu,

Kazuya Ishikawa, K. Tsuchiya

et al.

Infection and Immunity, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Animal infection models are essential for understanding bacterial pathogenicity and corresponding host immune responses. In this study, we investigated whether juvenile Xenopus laevis could be used as an model human pathogenic bacteria. frogs succumbed to intraperitoneal injection containing the bacteria Staphylococcus aureus, Pseudomonas aeruginosa, Listeria monocytogenes. contrast, non-pathogenic Bacillus subtilis Escherichia coli did not induce mortality in frogs. The administration of appropriate antibiotics suppressed caused by S. aureus P. aeruginosa. Strains lacking agr locus, cvfA (rny) gene, or hemolysin genes LIPI-1-deleted mutant L. monocytogenes, which attenuate virulence within mammals, exhibited reduced compared with their respective wild-type counterparts. Bacterial distribution analysis revealed that persisted blood, liver, heart, muscles until death. These results suggested induces sepsis-like symptoms frogs, supporting use a valuable animal evaluating antimicrobial efficacy identifying various

Language: Английский

Citations

0