Engineering extracellular vesicles for ROS scavenging and tissue regeneration DOI Creative Commons
Ahmed Abdal Dayem,

Ellie Yan,

Minjae Do

et al.

Nano Convergence, Journal Year: 2024, Volume and Issue: 11(1)

Published: June 26, 2024

Abstract Stem cell therapy holds promise for tissue regeneration, yet significant challenges persist. Emerging as a safer and potentially more effective alternative, extracellular vesicles (EVs) derived from stem cells exhibit remarkable abilities to activate critical signaling cascades, thereby facilitating repair. EVs, nano-scale membrane vesicles, mediate intercellular communication by encapsulating diverse cargo of proteins, lipids, nucleic acids. Their therapeutic potential lies in delivering cargos, activating pathways, efficiently mitigating oxidative stress—an essential aspect overcoming limitations cell-based This review focuses on engineering applying EVs emphasizing their role regulating reactive oxygen species (ROS) pathways. Additionally, we explore strategies enhance EV activity, including functionalization incorporation antioxidant defense proteins. Understanding these molecular mechanisms is crucial optimizing EV-based regenerative therapies. Insights into ROS modulation pave the way targeted efficient therapies harnessing EVs.

Language: Английский

Preconditioning and Engineering Strategies for Improving the Efficacy of Mesenchymal Stem Cell-Derived Exosomes in Cell-Free Therapy DOI Creative Commons
Shenyuan Chen, Fengtian Sun, Hui Qian

et al.

Stem Cells International, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 18

Published: May 14, 2022

Mesenchymal stem cells (MSCs) have been widely applied to regenerative medicine owing their multiple differentiation, self-renewal, and immunomodulatory abilities. Exosomes are cell-secreted natural nanovesicles thought be mediators of intercellular communication material transport. The therapeutic potential MSCs can largely attributed MSC-derived exosomes (MSC-exosomes). Emerging evidence suggests that the efficacy MSC-exosomes is highly dependent on status MSCs, optimization extracellular environment affects exosomal content. Pretreatment methods including three-dimensional cultures, hypoxia, other biochemical cues shown potentially enhance biological activity while maintaining or enhancing production. On hand, engineering means desired function has rapidly gaining attention. In particular, biologically active molecule encapsulation membrane modification alter functions targeting MSC-exosomes. this review, we summarize two possible strategies improve MSC-exosomes: preconditioning approaches exosomes. We also explore underlying mechanisms different discuss advantages limitations upcoming clinical applications.

Language: Английский

Citations

73
Extracellular vesicles in nanomedicine and regenerative medicine: A review over the last decade DOI Creative Commons
Saeid Moghassemi, Arezoo Dadashzadeh, Maria João Sousa

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 36, P. 126 - 156

Published: March 2, 2024

Small extracellular vesicles (sEVs) are known to be secreted by a vast majority of cells. These sEVs, specifically exosomes, induce specific cell-to-cell interactions and can activate signaling pathways in recipient cells through fusion or interaction. nanovesicles possess several desirable properties, making them ideal for regenerative medicine nanomedicine applications. properties include exceptional stability, biocompatibility, wide biodistribution, minimal immunogenicity. However, the practical utilization particularly clinical settings at large scale, is hindered expensive procedures required their isolation, limited circulation lifetime, suboptimal targeting capacity. Despite these challenges, sEVs have demonstrated remarkable ability accommodate various cargoes found extensive applications biomedical sciences. To overcome limitations broaden potential applications, researchers should strive deepen understanding current loading, characterization techniques. Additionally, acquiring fundamental knowledge about origins employing state-of-the-art methodologies expand research scope. This review provides comprehensive overview exosome-based strategies diverse domains, encompassing cancer therapy, immunotherapy, biomarker Furthermore, we emphasize immense exosomes medicine.

Language: Английский

Citations

31
Hydrogel-exosome system in tissue engineering: A promising therapeutic strategy DOI Creative Commons

Ming‐Hui Fan,

Jin-Kui Pi,

Chen‐Yu Zou

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 38, P. 1 - 30

Published: April 23, 2024

Characterized by their pivotal roles in cell-to-cell communication, cell proliferation, and immune regulation during tissue repair, exosomes have emerged as a promising avenue for "cell-free therapy" clinical applications. Hydrogels, possessing commendable biocompatibility, degradability, adjustability, physical properties akin to biological tissues, also found extensive utility engineering regenerative repair. The synergistic combination of hydrogels holds the potential not only enhance efficiency but collaboratively advance repair process. This review has summarized advancements made over past decade research hydrogel-exosome systems regenerating various tissues including skin, bone, cartilage, nerves tendons, with focus on methods encapsulating releasing within hydrogels. It critically examined gaps limitations current research, whilst proposed future directions applications this innovative approach.

Language: Английский

Citations

22
Hydrogels for Exosome Delivery in Biomedical Applications DOI Creative Commons
Yaxin Xie,

Qiuyue Guan,

Jiusi Guo

et al.

Gels, Journal Year: 2022, Volume and Issue: 8(6), P. 328 - 328

Published: May 24, 2022

Hydrogels, which are hydrophilic polymer networks, have attracted great attention, and significant advances in their biological biomedical applications, such as for drug delivery, tissue engineering, models medical studies, been made. Due to similarity physiological structure, hydrogels highly compatible with extracellular matrices tissues can be used both carriers encapsulate cellular secretions. As small vesicles secreted by nearly all mammalian cells mediate cell–cell interactions, exosomes play very important roles therapeutic approaches disease diagnosis. To maintain activity achieve controlled release, a strategy that embeds composite system has focused on recent studies. Therefore, this review aims provide thorough overview of the use embedding including resources making properties hydrogels, strategies combination exosomes.

Language: Английский

Citations

48
Mesenchymal Stem Cell-Derived Extracellular Vesicles for Bone Defect Repair DOI Creative Commons
Dongxue Wang, Hong Cao,

Weizhong Hua

et al.

Membranes, Journal Year: 2022, Volume and Issue: 12(7), P. 716 - 716

Published: July 19, 2022

The repair of critical bone defects is a hotspot orthopedic research. With the development tissue engineering (BTE), there increasing evidence showing that combined application extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) (MSC-EVs), especially exosomes, with hydrogels, scaffolds, and other bioactive materials has made great progress, exhibiting good potential for regeneration. Recent studies have found miRNAs, proteins, cargo loaded in EVs are key factors promoting osteogenesis angiogenesis. In BTE, expression profile intrinsic can be changed by modifying gene MSCs to obtain enhanced osteogenic activity ultimately enhance osteoinductive ability graft materials. However, current research on MSC-EVs repairing still its infancy, underlying mechanism remains unclear. Therefore, this review, effect such as hydrogels scaffolds summarized, defect delivering active molecules internal miRNAs further elucidated, which provides theoretical basis reference clinical defects.

Language: Английский

Citations

43
Single‐Cell and Spatial Transcriptomics Decodes Wharton's Jelly‐Derived Mesenchymal Stem Cells Heterogeneity and a Subpopulation with Wound Repair Signatures DOI
Penghong Chen, Shijie Tang, Ming Li

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 10(4)

Published: Dec. 11, 2022

Abstract The highly heterogeneous characteristics of Wharton's jelly mesenchymal stem cells (WJ‐MSCs) may be responsible for the poor clinical outcomes and reproducibility treatments based on WJ‐MSCs. Exploration WJ‐MSC heterogeneity with multimodal single‐cell technologies will aid in establishing accurate MSC subtyping developing screening protocols dominant functional subpopulations. Here, WJ‐MSCs are systematically analyzed by single cell spatial transcriptome sequencing. Single‐cell transcriptomics analysis identifies four subpopulations, namely proliferative_MSCs, niche‐supporting_MSCs, metabolism‐related_MSCs biofunctional‐type_MSCs. Furthermore, transcriptome, cellular heterogeneity, cell‐state trajectories these subpopulations characterized. Intriguingly, biofunctional‐type MSCs (marked S100A9, CD29, CD142) selected this study exhibit promising wound repair properties vitro vivo. Finally, integrating omics data, it has been found that S100A9 + CD29 CD142 subpopulation is more enriched fetal segment umbilical cord, suggesting deriving from have potential into an ideal therapeutic agent healing. Overall, presented comprehensively maps provides essential resource future development WJ‐MSC‐based drugs.

Language: Английский

Citations

42
3D-exosomes laden multifunctional hydrogel enhances diabetic wound healing via accelerated angiogenesis DOI
Han Peng, Huichen Li, Xi Zhang

et al.

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 475, P. 146238 - 146238

Published: Sept. 23, 2023

Language: Английский

Citations

36
Production and Utility of Extracellular Vesicles with 3D Culture Methods DOI Creative Commons
Mar Casajuana Ester, Richard M. Day

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(2), P. 663 - 663

Published: Feb. 16, 2023

In recent years, extracellular vesicles (EVs) have emerged as promising biomarkers, cell-free therapeutic agents, and drug delivery carriers. Despite their great clinical potential, poor yield unscalable production of EVs remain significant challenges. When using 3D culture methods, such scaffolds bioreactors, large numbers cells can be expanded the cell environment manipulated to control phenotype. This has been employed successfully increase well enhance effects. The physiological relevance cultures, spheroids, also provided a strategy for understanding role in pathogenesis several diseases evaluate tools deliver drugs. Additionally, methods encapsulate achieve more sustained effects prevent premature clearance enable localised concentrated exosome dosage. review highlights opportunities drawbacks different use EV research.

Language: Английский

Citations

31
Current Strategies for Promoting the Large-scale Production of Exosomes DOI
Qing Qu, Bin Fu,

Yong Long

et al.

Current Neuropharmacology, Journal Year: 2023, Volume and Issue: 21(9), P. 1964 - 1979

Published: Feb. 17, 2023

Exosomes, as nanoscale biological vesicles, have been shown to great potential for biomedical applications. However, the low yield of exosomes limits their application. In this review, we focus on methods increase exosome yield. Two main strategies are used production, one is based genetic manipulation biogenesis and release pathway, other by pretreating parent cells, changing culture method or adding different components medium. By applying these strategies, can be produced a large scale facilitate practical application in clinic.

Language: Английский

Citations

25
Multifunctional hydrogel-based engineered extracellular vesicles delivery for complicated wound healing DOI Creative Commons
Zuhao Li,

Jinlong Liu,

Jian Song

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(11), P. 4198 - 4217

Published: Jan. 1, 2024

The utilization of extracellular vesicles (EVs) in wound healing has been well-documented.However, the direct administration free EVs via subcutaneous injection at sites may result rapid dissipation bioactive components and diminished therapeutic efficacy.Functionalized hydrogels provide effective protection, as well ensure sustained release bioactivity during process, making them an ideal candidate material for delivering EVs.In this review, we introduce mechanisms by which accelerate healing, then elaborate on construction strategies engineered EVs.Subsequently, discuss synthesis application delivery systems to enhance complicated healing.Furthermore, face wounds, functionalized with specific microenvironment regulation capabilities, such antimicrobial, anti-inflammatory, immune regulation, used loading EVs, potential approaches addressing these challenges.Ultimately, deliberate future trajectories outlooks, offering a fresh viewpoint advancement artificial intelligence (AI)-energized materials 3D bio-printed multifunctional hydrogel-based dressings biomedical applications.

Language: Английский

Citations

15