Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: July 12, 2024
Abstract
Peripheral
nerve
injury
is
a
prevalent
clinical
problem
that
often
leads
to
lifelong
disability
and
reduced
quality
of
life.
Although
peripheral
nerves
can
regenerate,
recovery
after
severe
slow
incomplete.
The
current
gold
standard
treatment,
autologous
transplantation,
has
limitations
including
donor
site
morbidity
poor
functional
outcomes,
highlighting
the
need
for
improved
repair
strategies.
We
developed
reproducible
in
vitro
hollow
channel
collagen
gel
construct
investigate
regeneration
(PNR)
by
exploring
influence
key
extracellular
matrix
(ECM)
proteins
on
axonal
growth
regeneration.
Channels
were
coated
with
ECM
proteins:
IV,
laminin,
or
fibronectin
seeded
dorsal
root
ganglia
(DRG)
collected
from
E16
rat
embryos
compare
ability
enhance
growth.
Robust
extension
Schwann
cell
(SC)
infiltration
observed
fibronectin-coated
channels,
suggesting
its
superiority
over
other
proteins.
Differential
effects
axons
SCs
indicated
direct
stimulation
beyond
SC-mediated
guidance.
In
laceration
modeling
further
confirmed
fibronectin’s
superior
pro-regenerative
effects,
showcasing
potential
enhancing
regrowth
post-injury.
Advancing
closely
replicates
native
microenvironments
will
accelerate
progress
overcoming
approaches.
Tissue Engineering Part B Reviews,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 25, 2025
Electric
fields
(EFs)
offer
a
powerful
tool
for
manipulating
cells
and
modulating
their
behavior,
holding
significant
promise
regenerative
medicine
cell
biology.
We
provide
comprehensive
overview
of
the
effects
different
types
EF
on
eukaryotic
with
special
focus
physical
mechanisms
signaling
pathways
involved.
Direct
current
induces
electrophoresis
electroosmosis,
influencing
migration,
proliferation,
differentiation.
Alternating
EF,
through
dielectric
polarization
dielectrophoresis,
enables
manipulation,
trapping,
sorting.
Pulsed
particularly
high-intensity,
short-duration
pulses,
reversible
irreversible
electroporation,
facilitating
drug
gene
delivery.
The
review
covers
some
technological
aspects
generation,
emphasizing
importance
experimental
setups,
integration
microfluidic
platforms
high-throughput
analysis
precise
manipulations.
Furthermore,
synergistic
potential
combining
EFs
optical
tweezers
is
highlighted,
enabling
fine-tuned
control
positioning,
intercellular
interactions,
measurement
biophysical
properties.
Finally,
addresses
limitations
application,
such
as
field
heterogeneity
side
effects,
outlines
directions
future
studies,
including
developing
minimally
invasive
delivery
methods.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: July 12, 2024
Abstract
Peripheral
nerve
injury
is
a
prevalent
clinical
problem
that
often
leads
to
lifelong
disability
and
reduced
quality
of
life.
Although
peripheral
nerves
can
regenerate,
recovery
after
severe
slow
incomplete.
The
current
gold
standard
treatment,
autologous
transplantation,
has
limitations
including
donor
site
morbidity
poor
functional
outcomes,
highlighting
the
need
for
improved
repair
strategies.
We
developed
reproducible
in
vitro
hollow
channel
collagen
gel
construct
investigate
regeneration
(PNR)
by
exploring
influence
key
extracellular
matrix
(ECM)
proteins
on
axonal
growth
regeneration.
Channels
were
coated
with
ECM
proteins:
IV,
laminin,
or
fibronectin
seeded
dorsal
root
ganglia
(DRG)
collected
from
E16
rat
embryos
compare
ability
enhance
growth.
Robust
extension
Schwann
cell
(SC)
infiltration
observed
fibronectin-coated
channels,
suggesting
its
superiority
over
other
proteins.
Differential
effects
axons
SCs
indicated
direct
stimulation
beyond
SC-mediated
guidance.
In
laceration
modeling
further
confirmed
fibronectin’s
superior
pro-regenerative
effects,
showcasing
potential
enhancing
regrowth
post-injury.
Advancing
closely
replicates
native
microenvironments
will
accelerate
progress
overcoming
approaches.
