3D Printing of Polysaccharide-Based Hydrogel Scaffolds for Tissue Engineering Applications: A Review

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 270, P. 132123 - 132123

Published: May 17, 2024

Language: Английский

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Rapid Customization of Biomimetic Cartilage Scaffold with Stem Cell Capturing and Homing Capabilities for In Situ Inductive Regeneration of Osteochondral Defects

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(28)

Published: May 31, 2024

Abstract 3D printing of articular cartilage tissue faces challenges like replicating its complex structure, time‐consuming in vitro stem cell culture, and a lack robust situ regeneration methods for osteochondral defects (OC). In response, an innovative approach utilizing pre‐designed bioink modular units one‐step immediate implantation is proposed, circumventing the need prior cultivation. The resulting printed scaffold not only accurately reproduces three‐layer structure material gradient but also attains impressive compressive strength (6.3 MPa) through reinforcement hydroxyapatite nanofibers establishment chemical bonds with hydrogels. Moreover, integrates capturing homing layers on bottom top via crosslinking aptamer loading poly (lactic‐co‐glycolic acid) (PLGA) nanospheres encapsulated stromal cell‐derived factor‐1α (SDF‐1α), respectively. This design enables specific capture bone marrow mesenchymal cells (BMSCs) vivo interaction, followed by their mobilization to home hyaline layer chemotaxis SDF‐1α concentration gradient. Within scaffold's microenvironment, these BMSCs undergo differentiation into distinct each layer, effectively contributing repair OC rabbits.

Language: Английский

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Biomimetic fabrication bioprinting strategies based on decellularized extracellular matrix for musculoskeletal tissue regeneration: Current status and future perspectives

Materials & Design, Journal Year: 2024, Volume and Issue: 243, P. 113072 - 113072

Published: June 6, 2024

Musculoskeletal disorders, as one of the prevalent categories ailments, exert significant impacts on individuals' lives, occupations, and physical activities. Degenerative changes, injuries, infections, tumor resections causing defects in musculoskeletal tissues such cartilage, bones, skeletal muscles, menisci, ligaments, rotator cuffs can detrimentally affect patients' quality life mental well-being. Traditional autologous allogeneic transplantations have been clinically employed. However, transplantation suffers from limitation a finite number transplantable tissues, while faces challenges immune rejection. The extracellular matrix (ECM) serves natural scaffold for cells to fulfill physiological functions adhesion, proliferation, differentiation. Decellularized (dECM) emerges promising biomaterial generated through specific tissue or organ decellularization. Leveraging 3D bioprinting technology, dECM-based biomaterials enable customized printing construction. This study reviews various decellularization techniques, post-decellularization strategies, commonly used technologies. It summarizes integration with technology applied system research. These investigations showcase exciting potential system, offering prospects clinical translation orthopedics.

Language: Английский

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Filament Disturbance and Fusion during Embedded 3D Printing of Silicones

ACS Biomaterials Science & Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 5, 2024

Embedded 3D printing (EMB3D) is an additive manufacturing technique that enables complex fabrication of soft materials including tissues and silicones. In EMB3D, a nozzle writes continuous filaments into support bath consisting yield stress fluid. Lack fusion defects between can occur because the pushes fluid existing filaments, preventing coalescence. Interfacial tension was previously proposed as tool to drive interfilament fusion. However, interfacial also rupture shrinkage printed filaments. Here, we evaluate efficacy control in EMB3D. Using polydimethylsiloxane (PDMS)-based inks with varying amounts fumed silica surfactant, Laponite water supports, effect rheology, tension, print speeds, spacings on defects. We pairs parallel at orientations use digital image analysis quantify shrinkage, rupture, fusion, positioning By comparing disturbed disentangle effects movement filament extrusion. Critically, find capillary instabilities scale balance rheology tension. Less viscous supports higher tensions lead more all points process, from relaxation after writing, disturbance line, writing second line. It necessary overextrude material achieve particularly high viscosities low tensions. Finally, quality varies orientation, neighboring causes displacement structures. As such, specialized slicers are needed for EMB3D consider tighter orientation-dependent precise over shapes.

Language: Английский

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Emerging Trends in Bioprinting for Cartilage Regeneration: Materials, Techniques and Challenges

Pakistan BioMedical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 02 - 10

Published: Jan. 31, 2025

Cartilage repair is a major clinical problem because of the poor intrinsic healing capacity cartilage coupled with limitations conventional therapies and synthetic substitutes. These challenges have been pursued by bioprinting, which technique that can generate scaffolds mimic native cartilage. This review aims to discuss current future development bioprinting for tissue regeneration focus on most common biomaterials such as alginate, gelatin, collagen, along emerging materials smart hydrogels, nanomaterials, bioactive molecules. The also outlines other technologies like high resolution, 4D, hybrid, microfluidic assisted are believed improve mechanical properties, biological integration vascularization constructs produced through bioprinting. Some problems still unresolved those scale up, biocompatibility immune response hinders application bioprinted further concludes owing some regulatory issues lack an ideal practice in persists. prospects highlighted include use patient derived cells, artificial intelligence process optimization adaptive biomaterials. Mitigating these challenged integrated advanced will enable translation develop personalized, functional, durable constructs.

Language: Английский

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Regenerative Medicine in Plastic Surgery: The Role of Stem Cells and Bioprinting

Regenesis repair rehabilitation., Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

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Nanotechnology-Assisted mesenchymal stem cells treatment for improved cartilage regeneration: A review of current practices

Biochemical Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 116895 - 116895

Published: March 1, 2025

Language: Английский

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Therapeutic potential of apoptotic vesicles in modulating inflammation, immune responses, and tissue regeneration

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 1, 2025

Abstract The process of apoptosis plays a crucial role in tissue homeostasis, immune system regulation, and organ formation. Apoptotic vesicles (ApoEVs) are involved efferocytosis, the by which phagocytes ingest dead cells. ApoEVs also have potential therapeutic applications cancer treatment, ischemic diseases, their anti-inflammatory properties make them incredibly versatile for medical applications. These can induce cells, provide tumor antigens vaccines, even serve as effective drug delivery systems. Moreover, they target hypoxic inhibit inflammatory cell death pathways, promote regeneration. Also, addressing disorders such gastrointestinal ailments, osteoarthritis, diabetes is promising. Additionally, polarize cells suppress responses viable option unmet need novel medications. Despite wealth reviews examining ApoEVs, very few thoroughly investigated mechanisms underlying effects. This distinctive approach positions current review timely immensely relevant, illuminating intriguing ways these entities function beyond established advantages. Graphical

Language: Английский

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Bioprinting in Organ Transplantation: From Experimental Models to Clinical Prospects

Brazilian Journal of Transplantation, Journal Year: 2025, Volume and Issue: 28(1)

Published: April 25, 2025

Background: Bioprinting has emerged as an innovative technology in organ transplantation and regenerative medicine, aiming to address pressing challenges such the shortage of donor organs need for effective tissue repair. By leveraging advanced 3D printing techniques, bioprinting enables fabrication functional tissues with precise architectural biological properties. Methods: This review provides in-depth analysis latest advancements bioprinting, focusing on cutting-edge development bioinks, their applications engineering. It examines significant breakthroughs creation vascularized transplantable prototypes explores role personalized medicine. Results: The findings highlight transformative impact biomedical field, particularly drug testing, therapeutic modeling, patient-specific treatment strategies. Additionally, key challenges—including technological limitations, ethical concerns, regulatory considerations—are discussed provide a comprehensive understanding field’s progress potential obstacles. Conclusion: holds immense promise revolutionizing global healthcare by offering solutions shortages advancing However, continued research innovation are necessary overcome existing facilitate its clinical translation into mainstream medical practice.

Language: Английский

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Bioimpressão no Transplante de Órgãos: Dos Modelos Experimentais às Perspectivas Clínicas

Brazilian Journal of Transplantation, Journal Year: 2025, Volume and Issue: 28(1)

Published: April 25, 2025

Introdução: A bioimpressão despontou como uma tecnologia inovadora no transplante de órgãos e na medicina regenerativa, visando solucionar desafios urgentes, a escassez doadores necessidade reparo eficaz tecidos. Ao aproveitar as técnicas avançadas impressão 3D, permite fabricação tecidos funcionais com propriedades arquitetônicas biológicas precisas. Métodos: Esta revisão fornece análise detalhada dos últimos avanços em bioimpressão, concentrando-se ponta, desenvolvimento biotintas suas aplicações engenharia Ela examina os significativos criação protótipos vascularizados transplantáveis explora função da personalizada. Resultados: As descobertas destacam o impacto transformador campo biomédico, particularmente testes medicamentos, modelagem terapêutica estratégias tratamento específicas para paciente. Além disso, principais — incluindo limitações tecnológicas, preocupações éticas considerações regulatórias são discutidos fornecer compreensão abrangente do progresso possíveis obstáculos. Conclusão: é imensamente promissora revolucionar saúde global, oferecendo soluções avanço regenerativa. No entanto pesquisa inovação contínuas necessárias superar existentes facilitar sua tradução clínica prática médica convencional.

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