Lithium-doped calcium silicate cement regulates the immune microenvironment and promotes M2 macrophage polarization for enhancing bone regeneration

Journal of Biological Engineering, Journal Year: 2025, Volume and Issue: 19(1)

Published: Jan. 6, 2025

Bone defects present a significant challenge in orthopedics and trauma surgery, necessitating innovative approaches to stimulate effective bone regeneration. This study investigated the potential of lithium-doped calcium silicate (LiCS) cement enhance regeneration modulate immune microenvironment promote tissue repair. We synthesized LiCS ceramic powder performed comprehensive analyses its physicochemical properties, including phase composition, morphology, setting time, mechanical strength. The results demonstrated that incorporation lithium into significantly increased diametral tensile strength (DTS) facilitated hydroxyapatite formation compared with undoped silicate. In vitro assays revealed enhanced proliferation, adhesion, spread Wharton's jelly mesenchymal stem cells (WJMSCs). Additionally, Li-CS exhibited remarkable immunomodulatory properties by reducing pro-inflammatory cytokines increasing anti-inflammatory cytokines, promoting polarization macrophages towards M2 phenotype. presence Li also improved osteogenic differentiation WJMSCs, as evidenced elevated levels alkaline phosphatase osteocalcin expression. These findings underscore dual functional capabilities enhancing osteogenesis modulating environment, making it promising material for engineering

Language: Английский

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Material‐Mediated Immunotherapy to Regulate Bone Aging and Promote Bone Repair

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

Abstract As the global population ages, an increasing number of elderly people are experiencing weakened bone regenerative capabilities, resulting in slower repair processes and associated risks various complications. This review outlines research progress on biomaterials that promote through immunotherapy. examines how manufacturing technologies such as 3D printing, electrospinning, microfluidic technology contribute to enhancing therapeutic effects these biomaterials. Following this, it provides detailed introductions anti‐osteoporosis drug delivery systems, injectable hydrogels, nanoparticles, engineered exosomes, well tissue engineering materials coatings used immunomodulation. Moreover, critically analyzes current limitations biomaterial‐mediated immunotherapy explores future directions for material‐mediated aims inspire new approaches broaden perspectives addressing challenges aging by exploring innovative strategies.

Language: Английский

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Crosstalk Between H-Type Vascular Endothelial Cells and Macrophages: A Potential Regulator of Bone Homeostasis

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 2743 - 2765

Published: Feb. 1, 2025

The crosstalk between H-type endothelial cells (ECs) and macrophages is critical for maintaining angiogenesis osteogenesis in bone homeostasis. As core components of type H vessels, ECs respond to various pro-angiogenic signals, forming specialized vascular structures characterized by high expression platelet-endothelial cell adhesion molecule-1 (CD31) mucin (EMCN), thereby facilitating angiogenesis-osteogenesis coupling during formation. Macrophages, as key immune the perivascular region, are primarily classified into classically activated pro-inflammatory M1 phenotype selectively anti-inflammatory M2 phenotype, performing dual functions regulating local tissue homeostasis innate immunity. In recent years, complex vessel has garnered significant interest context bone-related diseases. Orderly regulation immunity provides a new direction preventing metabolic disorders such osteoporosis osteoarthritis. However, their interactions remain insufficiently understood, with limited clinical data available. This review comprehensively examines intricate diverse phenotypes, Insights signaling pathways that regulate crosstalk, focusing on roles osteogenesis. Furthermore, discusses interventions targeting this challenges remain. These insights may offer perspectives provide theoretical foundation developing novel therapeutic strategies.

Language: Английский

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Fibroblast IRF7-mediated chondrocyte apoptosis affects the progression of collapse in steroid-induced osteonecrosis of the femoral head

Journal of Orthopaedic Surgery and Research, Journal Year: 2025, Volume and Issue: 20(1)

Published: March 18, 2025

The objective of this study was to identify potential genes implicated in the "peri-collapse" synovium osteonecrosis femoral head through coding gene sequencing and further clarify their specific mechanisms via vitro experiments. Steroid-induced (SIONFH) (n = 3), neck fracture (FNF) hip osteoarthritis (HOA) 3) Synovial tissue joint collected total arthroplasty. A cellular model SIONFH constructed from rat synovial fibroblasts by lipopolysaccharide intervention. Lentiviral technology used construct a for fibroblast knockout Irf7 gene. HE compare characteristics damage, immunofluorescence immunohistochemistry were expression levels VIM, IRF7, IFNα. PCR, WB, IF examine knockdown efficiency, chondrocyte proliferation (Col2a1, Aggrecan, Sox9), cartilage matrix degradation (Mmp13), apoptosis (Bcl2, Bax, Caspase3) under co-culture conditions. Crystalline violet staining observe migration rate fibroblasts, flow cytometry detect level chondrocytes Transcriptome ultimately screened six differential genes, HOOK1, RNPC3, KCNA3, CD48, SAMD9. Compared FNF HOA, inflammatory cell recruitment hyperplasia more pronounced SIONFH. IHC confirmed high IRF7 IFNα PCR WB results suggested that highly expressed Irf7, Hook1, Rnpc3, Kcna3, Cd48, Samd9, Il-6, Tnfα after intervention, Il-6 significantly reduced (P < 0.001). In system, intervened with promoted apoptosis, degradation, while inhibiting proliferation, result reversed fibroblasts. This supported results. SAMD9 as affecting progression collapse. mediates response affects collapse process influencing apoptosis. Thus, intervention holds promise one key targets reversing

Language: Английский

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Low‐crystalline magnesium‐doped carbonate apatite/β‐tricalcium phosphate granules from sea urchin spine

Journal of the American Ceramic Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 22, 2025

Abstract The development of synthetic granules with performances equivalent to those autogenous bone grafts is critical for effective replacement. Herein, we report on the fabrication low‐crystalline Mg‐doped biphasic carbonate apatite/β‐tricalcium phosphate (Mg‐CAp/β‐TCP) granules, cylindrical shapes, concave surfaces, and multiscale pores, from sea urchin spines. β‐TCP/CAp ratios Mg contents in Mg‐CAp/β‐TCP were controlled remain within 0.46–0.87 6.03–9.32 mol%, respectively. preserved pores surfaces exhibited inner stereom micropores (3–25 µm), thickened (<14 interseptal gaps (20–43 concavities (26–41 along (<3 µm) nanopores (<100 nm) corresponding intercrystal spaces. rounded uniformly shaped facilitated denser packing a simulated defect mold. compressive diametral tensile strengths 1.12–1.51 0.17–0.21 MPa, distinctive physicochemical structural characteristics can be anticipated facilitate resorption rates promote osteogenesis angiogenesis, facilitating rapid repair. Furthermore, adequate mechanical features enable ease handling during filling potential application load‐bearing sites.

Language: Английский

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Dual-functional injectable nanofiber hydrogels with anti-inflammatory and osteogenic effects for bone regeneration

International Journal of Polymeric Materials, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 17

Published: March 24, 2025

Language: Английский

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Regulation of macrophage polarization and pyroptosis by 4-methylcatechol alleviates collagen-induced arthritis via Nrf2/HO-1 and NF-κB/NLRP3 signaling pathways

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 146, P. 113855 - 113855

Published: Dec. 21, 2024

Language: Английский

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Biomaterial Cues for Regulation of Osteoclast Differentiation and Function in Bone Regeneration

Advanced Therapeutics, Journal Year: 2024, Volume and Issue: 8(1)

Published: Nov. 15, 2024

Abstract Tissue regeneration involves dynamic dialogue between and among different cells their surrounding matrices. Bone is specifically governed by reciprocity osteoblasts osteoclasts within the bone microenvironment. Osteoclast‐directed resorption osteoblast‐directed formation of are essential to remodeling, crosstalk these vital curating a sequence events that culminate in creation tissue. Among biomaterial strategies, many have investigated use material cues direct development activity osteoblasts. However, less attention has been given exploring features similarly target osteoclast activity, with even fewer strategies demonstrating or integrating biomaterial‐directed modulation osteoblast‐osteoclast coupling. This review aims describe various demonstrated influence osteoclastogenesis function, emphasizing those enhance construct's ability achieve healing regeneration. Additionally discussed approaches communication osteoblasts, particularly manner takes advantage Deepening understanding how may dictate differentiation, on microenvironment enable realization bone‐replacement interventions enhanced integrative regenerative capacities.

Language: Английский

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Lithium-doped calcium silicate regulates the immune microenvironment and promotes M2 macrophage polarization for enhancing bone regeneration

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 11, 2024

Bone defects present a significant challenge in orthopedics and trauma surgery, necessitating innovative approaches to stimulate effective bone regeneration. This study investigated the potential of lithium-doped calcium silicate (LiCS) cement enhance regeneration modulate immune microenvironment promote tissue repair. We synthesized LiCS ceramic powder performed comprehensive analyses its physicochemical properties, including phase composition, morphology, setting time, mechanical strength. The results demonstrated that incorporation lithium into significantly increased diametral tensile strength (DTS) facilitated hydroxyapatite formation compared with undoped silicate. In vitro assays revealed enhanced proliferation, adhesion, spread Wharton's jelly mesenchymal stem cells (WJMSCs). Additionally, Li-CS exhibited remarkable immunomodulatory properties by reducing pro-inflammatory cytokines increasing anti-inflammatory cytokines, promoting polarization macrophages towards M2 phenotype. presence Li also improved osteogenic differentiation WJMSCs, as evidenced elevated levels alkaline phosphatase osteocalcin expression. These findings underscore dual functional capabilities enhancing osteogenesis modulating environment, making it promising material for engineering

Language: Английский

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Highly disordered and resorbable lithiated nanoparticles with osteogenic and angiogenic properties

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(38), P. 9575 - 9591

Published: Jan. 1, 2024

This study develops lithium-doped amorphous calcium phosphate nanoparticles. These bioresorbable particles promote osteogenesis and angiogenesis, effectively inducing the formation of mature ectopic bone.

Language: Английский

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