Ginsenoside Rb1 improves human nonalcoholic fatty liver disease with liver organoids-on-a-chip DOI Creative Commons
Hui Wang, Yue Zhu, Pengcheng Shi

et al.

Engineered Regeneration, Journal Year: 2024, Volume and Issue: 5(3), P. 283 - 294

Published: June 21, 2024

Non-alcoholic fatty liver disease (NAFLD), a type of for which no treatment is currently approved, remains major concern worldwide. It manifested as simple hepatocyte steatosis and can develop into inflammation, fibrosis, cirrhosis cancer in severe cases. However, due to the lack appropriate vitro drug testing platforms, an in-depth understanding therapeutic activity ginsenoside Rb1 NAFLD challenging. Here, we proposed model on organoids (LOs)-on-a-chip platform evaluate effect dynamic, multi-condition high-throughput manner. This allowed us reshape certain features such multicellular types liver-specific functions physiology human-relative liver. Free acids (FFAs)-induced LOs displayed typical pathological characteristics progression, including steatosis, oxidative stress, lipid peroxidation, inflammation fibrosis. With intervention, these be significantly improved, may provide new insights potential mechanisms progression suggest clinical implications humans. The system enables formation, differentiation, function serve scalable, sensitive model, potentially expedite discovery.

Language: Английский

Organoids-on-Chips Technology: Unveiling New Perspectives in Rare-Disease Research DOI Open Access
Xiangyang Li, Hui Wang, Xiaoyan You

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4367 - 4367

Published: May 4, 2025

The scarcity of robust models and therapeutic options for rare diseases continues to hamper their preclinical investigation. Traditional animal two-dimensional cell cultures are limited in ability replicate human heredity-associated traits complex pathological features. Organoids-on-a-chip approaches open up new frontiers rare-disease research via the integration organ chips organoid technology. This integrative strategy offers immense opportunities mimicry disease-related traits, clarification mechanisms underlying disease, prediction treatment responses a highly human-related manner. forward-looking perspective suggests organoids on transformative tools parsing pathogenesis, accelerating discovery, bridging gap between basic precision medicine.

Language: Английский

Citations

0
Coculture systems to study interactions between anaerobic bacteria and intestinal epithelium DOI
Lyudmila Boyanova, Raina Gergova, Rumyana Markovska

et al.

Anaerobe, Journal Year: 2025, Volume and Issue: unknown, P. 102949 - 102949

Published: Feb. 1, 2025

Language: Английский

Citations

0
Microfluidic organ-on-a-chip models for the gut–liver axis: from structural mimicry to functional insights DOI
Weifeng Hu, Yushen Wang,

Junlei Han

et al.

Biomaterials Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review discusses advancements in organ-on-a-chip models, emphasizing gut, liver, and integrated gut–liver systems. It covers their applications disease modeling, toxicity testing, drug screening, as well future prospects.

Language: Английский

Citations

0
Innovative microfluidic model for investigating the intestinal mucus barrier: numerical and experimental perspectives DOI Creative Commons
Mohammad Valibeknejad, Reza Alizadeh, S. Majid Abdoli

et al.

Drug Delivery and Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

The intestinal mucus layer serves as a critical first line of defense against external agents, functioning barrier to the absorption drugs, food, and pathogens. While numerous in vitro studies have explored role preventing particle penetration, effects flowing luminal material, dislodging because induced shear rate by lumen material interfacial phenomena remain poorly understood. This study introduces microfluidic approach simulate interaction between layer. successfully measures both penetration into dislodgement material. A biosimilar model (BSM) Hank's Balanced Salt Solution (HBSS) were employed mimics human fluid, respectively. To investigate effect viscosity on pattern, two variants used: BSM-1, representing low-viscosity model, BSM-2, high-viscosity model. velocity fields extracted tracking fluorescent particles. results revealed significant differences BSM-1 attributed their rheological properties. These findings further confirmed through an assessment viscoelastic properties BSM models. utilized COMSOL Multiphysics for numerical simulations, predicting experimental outcomes solving fluid flow equations. Physicochemical characterizations HBSS performed link with including sweep tests, application power-law viscosity, measurements density wettability. proposes platform examining low- offer valuable insights barrier's response stress. validated physicochemical provide foundation future rates more complex geometries diverse conditions.

Language: Английский

Citations

0
Intelligent Manufacturing for Osteoarthritis Organoids DOI Creative Commons
Xin Lyu, Jian Wang, Jiacan Su

et al.

Cell Proliferation, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

ABSTRACT Osteoarthritis (OA) is the most prevalent degenerative joint disease worldwide, imposing a substantial global burden. However, its pathogenesis remains incompletely understood, and effective treatment strategies are still lacking. Organoid technology, in which stem cells or progenitor self‐organise into miniature tissue structures under three‐dimensional (3D) culture conditions, provides promising vitro platform for simulating pathological microenvironment of OA. This approach can be employed to investigate mechanisms, carry out high‐throughput drug screening facilitate personalised therapies. review summarises structure, OA manifestations, thereby establishing context application organoid technology. It then examines components arthrosis system, specifically addressing cartilage, subchondral bone, synovium, skeletal muscle ligament organoids. Furthermore, it details various constructing organoids, including considerations cell selection, classification fabrication techniques. Notably, this introduces concept intelligent manufacturing organoids by incorporating emerging engineering technologies such as artificial intelligence (AI) process, forming an innovative software hardware cluster. Lastly, discusses challenges currently facing highlights future directions rapidly evolving field. By offering comprehensive overview state‐of‐the‐art methodologies challenges, anticipates that intelligent, automated will expedite fundamental research, discovery translational applications orthopaedic

Language: Английский

Citations

0
Alginate-gelatin hydrogel scaffolds for establishing physiological barriers on a gut-brain-axis microchip DOI
Gaowa Xing, Yuxuan Li, Zengnan Wu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 144084 - 144084

Published: May 1, 2025

Language: Английский

Citations

0
Ginsenoside Rb1 improves human nonalcoholic fatty liver disease with liver organoids-on-a-chip DOI Creative Commons
Hui Wang, Yue Zhu, Pengcheng Shi

et al.

Engineered Regeneration, Journal Year: 2024, Volume and Issue: 5(3), P. 283 - 294

Published: June 21, 2024

Non-alcoholic fatty liver disease (NAFLD), a type of for which no treatment is currently approved, remains major concern worldwide. It manifested as simple hepatocyte steatosis and can develop into inflammation, fibrosis, cirrhosis cancer in severe cases. However, due to the lack appropriate vitro drug testing platforms, an in-depth understanding therapeutic activity ginsenoside Rb1 NAFLD challenging. Here, we proposed model on organoids (LOs)-on-a-chip platform evaluate effect dynamic, multi-condition high-throughput manner. This allowed us reshape certain features such multicellular types liver-specific functions physiology human-relative liver. Free acids (FFAs)-induced LOs displayed typical pathological characteristics progression, including steatosis, oxidative stress, lipid peroxidation, inflammation fibrosis. With intervention, these be significantly improved, may provide new insights potential mechanisms progression suggest clinical implications humans. The system enables formation, differentiation, function serve scalable, sensitive model, potentially expedite discovery.

Language: Английский

Citations

1