Efficient Fabrication of Human Corneal Stromal Cell Spheroids and Promoting Cell Stemness Based on 3D-Printed Derived PDMS Microwell Platform DOI Creative Commons
Yuexi Chen, Jianing Gu, Zekai Cui

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 438 - 438

Published: March 19, 2025

Spherical culture could promote the plasticity and stemness of human corneal stromal cells (hCSCs). Here, we introduce a novel three-dimensional (3D) cell system based on polydimethylsiloxane (PDMS) microwell platform composed many V-bottom microcavities to generate spheroids stemness. We isolated hCSCs from SMILE-derived lenticules maintained their physiological phenotype by culturing them in medium supplemented with extract (hCSE). Utilizing PDMS fabricated through 3D printing technology, successfully generated (3D-CSC) uniform size stable structure, exhibiting increased expression pluripotency factors, including OCT4, NANOG, SOX2, KLF4, PAX6. Furthermore, iPS supernatant E8-conditioned (E8-CM) significantly enhanced properties these cells. RNA sequencing proteomics analyses revealed that 3D-CSCs exhibited superior proliferation, differentiation, adhesion, migration, neurogenesis compared traditional monolayer cultures, underscoring role biophysical cues promoting In summary, this study presents an effective mimics vivo microenvironment, facilitating enhancement providing valuable insights into tissue engineering regenerative medicine, particularly for treating opacities diseases.

Language: Английский

Efficient Fabrication of Human Corneal Stromal Cell Spheroids and Promoting Cell Stemness Based on 3D-Printed Derived PDMS Microwell Platform DOI Creative Commons
Yuexi Chen, Jianing Gu, Zekai Cui

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 438 - 438

Published: March 19, 2025

Spherical culture could promote the plasticity and stemness of human corneal stromal cells (hCSCs). Here, we introduce a novel three-dimensional (3D) cell system based on polydimethylsiloxane (PDMS) microwell platform composed many V-bottom microcavities to generate spheroids stemness. We isolated hCSCs from SMILE-derived lenticules maintained their physiological phenotype by culturing them in medium supplemented with extract (hCSE). Utilizing PDMS fabricated through 3D printing technology, successfully generated (3D-CSC) uniform size stable structure, exhibiting increased expression pluripotency factors, including OCT4, NANOG, SOX2, KLF4, PAX6. Furthermore, iPS supernatant E8-conditioned (E8-CM) significantly enhanced properties these cells. RNA sequencing proteomics analyses revealed that 3D-CSCs exhibited superior proliferation, differentiation, adhesion, migration, neurogenesis compared traditional monolayer cultures, underscoring role biophysical cues promoting In summary, this study presents an effective mimics vivo microenvironment, facilitating enhancement providing valuable insights into tissue engineering regenerative medicine, particularly for treating opacities diseases.

Language: Английский

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