Immunomodulatory properties of polysaccharide extract samples from Cyanobacterium sp. Rippka B-1200 DOI Creative Commons
Станислав Сухих, Vladimir O. Popov,

Egor Kashinskikh

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 5, 2024

Cyanobacteria are most abundant in aquatic systems and can grow freshwater, saline or brackish water, cold/hot springs. have attracted considerable research attention the last decade as a potential source of numerous biological products large quantities, such biofuels, pigments, polyunsaturated fatty acids, nutraceuticals, enzymes, polysaccharides. Unlike plant fungal polysaccharides, chemical composition, immunomodulatory activity, molecular mechanisms action Cyanobacterium sp. Rippka B-1200 polysaccharides been studied much less. The complexity their primary structure due to high variability monosaccharides, diverse bonds, presence substituents viscosity made detailed structural studies cyanobacterial rare, which determines need for analysis cyanobacteria biomass components identify active metabolites with promising activity. aim this study was investigate properties from B-1200. Pharmacological nutraceutical value has set, defining our study's scientific novelty. As result, weight immunoactive polysaccharide (6.0–8.0 kDa) determined. shows that endopolysaccharide samples at concentration 300 mg/kg showed no significant effect (1.60 ± 0.15 mg/g), thymus mass index animals experimental group comparable control were immunosuppressed cyclophosphamide (1.15 0.24 mg/g). When exopolysaccharide used 600 mg/kg, (3.60 0.32 mg/g) statistically (without polysaccharide) immunosuppression not induced (thymus 3.70 0.25 It found also exhibited used, internal organ changes observed after immunosuppression. empirical results presented may find application development both pharmaceutical cosmetic products.

Language: Английский

Cyanobacteria for environmental, energy and biomedical application: a review DOI

Xiaoya Ren,

Mengqi Feng,

Meiyi Mao

et al.

Environmental Chemistry Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 12, 2025

Language: Английский

Citations

0
Characterization of Bioactive Metabolites in Phormidium sp. PB21: Pigment Production, Antimicrobial Potential, and Toxicity Assessment DOI Open Access
Prasannabalaji Nainangu,

K Keerthiga,

Kumaran Subramanian

et al.

Chemistry & Biodiversity, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

ABSTRACT This study investigates the morphological characteristics, pigment production, antimicrobial activity, and toxicity of secondary metabolites from filamentous cyanobacterium Phormidium sp. PB21, isolated freshwater ecosystems. The strain demonstrated significant potential for bioactive compound as evidenced by its chlorophyll‐ a carotenoid production. Chlorophyll‐ levels increased at 0.071 mg/mL day 24, reflecting robust photosynthetic while content reached maximum 1.6 under controlled conditions, highlighting pigment‐based applications. Antimicrobial assays using methanol: chloroform (1:3) crude extracts revealed inhibition human pathogens, including Serratia (12 mm) Pseudomonas aeruginosa (11 mm), with minimum inhibitory concentrations low 15.6 µg/mL specific fractions. Toxicity assessment Artemia salina indicated dose‐dependent effects, LC 50 values 93 82 fractions 3 4, respectively, suggesting natural agents. gas chromatography‐high‐resolution mass spectrometry analysis sample identified key compounds, decanoic acid, 5‐acetoxytridecane, 1‐undecanol, based on their characteristic m/z values. These compounds exhibit antimicrobial, anti‐inflammatory, pharmacological properties, sample's promising therapeutic industrial findings emphasize biotechnological particularly in production

Language: Английский

Citations

0
Inducible Mtld expression facilitated the introduction of the mannitol synthesis pathway in Synechococcus elongatus PCC 7942 DOI Creative Commons
Jiahui Sun,

Jinyu Cui,

Xuejing Xu

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: March 21, 2025

Mannitol is a valuable sugar alcohol, extensively used across various industries. Cyanobacteria show potential as future platforms for mannitol production, utilizing CO 2 and solar energy directly. The proof-of-concept has been demonstrated by introducing two-step pathway in cyanobacteria, converting fructose-6-phosphate to mannitol-1-phosphate sequentially mannitol. However, recombinant strains generally faced issues of genetic instability or low titers, consequently affecting the long-term production. In this work, construction strategy engineering production Synechococcus elongatus PCC 7942, based on commonly adopted comprising dehydrogenase (Mtld) mannitol-1-phosphatase (M1Pase), was optimized. results that sequential introduction m1p mtld required obtain mannitol-producing strains. We further manipulated abundances Mtld with theophylline dose-responsive riboswitch approach, combining it overexpression , we successfully obtained strain producing 1.5 g/L under optimal conditions, highest cyanobacterial yield date. addition, controlled expression remarkably augment stability mutant culturing circumstances, which continued secrete after more than months cultivation without addition theophylline, biosynthesis operon did not undergo any spontaneous mutation. findings work provided novel insights into area cyanobacteria metabolism engineering, would inspire researchers construct different gene regulatory strategies efficient photosynthetic biosynthesis.

Language: Английский

Citations

0
Immunomodulatory properties of polysaccharide extract samples from Cyanobacterium sp. Rippka B-1200 DOI Creative Commons
Станислав Сухих, Vladimir O. Popov,

Egor Kashinskikh

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 5, 2024

Cyanobacteria are most abundant in aquatic systems and can grow freshwater, saline or brackish water, cold/hot springs. have attracted considerable research attention the last decade as a potential source of numerous biological products large quantities, such biofuels, pigments, polyunsaturated fatty acids, nutraceuticals, enzymes, polysaccharides. Unlike plant fungal polysaccharides, chemical composition, immunomodulatory activity, molecular mechanisms action Cyanobacterium sp. Rippka B-1200 polysaccharides been studied much less. The complexity their primary structure due to high variability monosaccharides, diverse bonds, presence substituents viscosity made detailed structural studies cyanobacterial rare, which determines need for analysis cyanobacteria biomass components identify active metabolites with promising activity. aim this study was investigate properties from B-1200. Pharmacological nutraceutical value has set, defining our study's scientific novelty. As result, weight immunoactive polysaccharide (6.0–8.0 kDa) determined. shows that endopolysaccharide samples at concentration 300 mg/kg showed no significant effect (1.60 ± 0.15 mg/g), thymus mass index animals experimental group comparable control were immunosuppressed cyclophosphamide (1.15 0.24 mg/g). When exopolysaccharide used 600 mg/kg, (3.60 0.32 mg/g) statistically (without polysaccharide) immunosuppression not induced (thymus 3.70 0.25 It found also exhibited used, internal organ changes observed after immunosuppression. empirical results presented may find application development both pharmaceutical cosmetic products.

Language: Английский

Citations

0