Bioinks for engineering gradient-based osteochondral and meniscal tissue substitutes: a review

Biofabrication, Journal Year: 2025, Volume and Issue: 17(2), P. 022005 - 022005

Published: Jan. 31, 2025

Gradient tissues are anisotropic structure with gradual transition in structural and biological properties. The gradient structural, mechanical biochemical properties of osteochondral meniscal play a major role defining tissue functions. Designing substitutes that replicate these is crucial to facilitate regeneration functions following injuries. Advanced manufacturing technologies such as 3D bioprinting hold great potentials for recreating nature through using zone-specific bioinks layer-by-layer deposition spatially defined biomaterials, cell types bioactive cues. This review highlighted the gradients detail, elaborated on individual components bioink, reviewed recent advancements gradient-based substitutes. Finally, key challenges field future perspectives developing were discussed. insights from advances can broaden possibilities engineering tissues.

Language: Английский

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Evaluation of Custom Microalgae-Based Bioink Formulations for Optimized Green Bioprinting

Materials, Journal Year: 2025, Volume and Issue: 18(4), P. 753 - 753

Published: Feb. 8, 2025

Green bioprinting, from the context of merging 3D bioprinting with microalgae cell organization, holds promise for industrial-scale optimization. This study employs spectrophotometric analysis to explore post-bioprinting growth density variation within hybrid hydrogel biomaterial scaffolds. Three biomaterials-Alginic acid sodium salt (ALGINATE), Nanofibrillated Cellulose (NFC)-TEMPO, and CarboxyMethyl (CMC)-are chosen their scaffolding capabilities. Bioink development impact on proliferation morphology are conducted. Chlorella compositions is probed using absorbance measurements, additional assessment shear thinning properties. Notably, NFC exhibits reduced compared CMC. Results reveal that while mono-hydrogel substrates pronounced adhesion inhibit proliferation, alginate fosters increased concentration alongside a slight viscosity rise.

Language: Английский

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Unleashing the Healing Power: 3D Bioprinting Mimics Hypoxia to Supercharge Mesenchymal Stem Cells

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: March 2, 2025

Mesenchymal stem cells (MSCs) play a critical role in cell therapy due to their tissue-mimicking abilities. However, conventional 2D culture conditions often lead the loss of native hypoxic niche, potentially limiting therapeutic efficacy. 3D bioprinting offers method recreate intricate biological environments by integrating with extracellular matrices. Therefore, it is essential adapt printing techniques accurately replicate MSCs' ecological facilitating integration technology into clinical applications. In this study, we optimized capabilities using performed cellular aggregates (PCA) method. We observed that printed matrix creates microenvironment, resulting significant increase level production several paracrine signaling molecules and immunomodulatory factors MSCs. Furthermore, MSCs exhibited enhanced stemness proliferative capacity early stages culture. RNA-seq analysis revealed these changes behavior were associated environment created during procedure By optimizing bioink composition parameters, successfully simulated vivo leading notable improvements MSC characteristics capacity. RNA sequencing further confirmed activation hypoxia pathways, which are crucial for properties. These findings offer valuable insights leveraging MSC-based therapies regenerative medicine.

Language: Английский

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Printable FET sensors with using GNH/MnO2 as channel material for non-enzymatic detection of bilirubin

Diamond and Related Materials, Journal Year: 2025, Volume and Issue: unknown, P. 112187 - 112187

Published: March 1, 2025

Language: Английский

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Advances in bioink-based 3D printed scaffolds: optimizing biocompatibility and mechanical properties for bone regeneration

Biomaterials Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The development of bioink-based 3D-printed scaffolds has revolutionized bone tissue engineering (BTE) by enabling patient-specific and biomimetic constructs for regeneration.

Language: Английский

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Conductive biological materials for in vitro models: properties and sustainability implications

In vitro models, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract The integration of conductive biological materials into in vitro models represents a transformative approach to advancing biomedical research while addressing critical sustainability challenges. Traditional used tissue engineering and disease modeling are often environmentally detrimental, derived from non-renewable resources, limited their ability replicate the dynamic properties native tissues. Conductive bridge this gap by offering unique combination biodegradability, sustainability, functional properties, such as bioelectricity biocompatibility, that essential for mimicking physiological environments. Herein, development current applications biodegradable materials, including advanced polymers polyaniline polypyrrole, carbon-based nanocomposites, renewable biopolymers lignin cellulose, overviewed. These not only reduce ecological footprint but also enable precise simulation electrical signaling tissues, cardiac, neural, muscular systems, thereby enhancing relevance models. Their three-dimensional (3D) constructs, organ-on-chip platforms, bioprinting technologies facilitates patient-specific models, paving way personalized therapeutic diagnostic applications. In addition precision, these align with global efforts implement circular economy principles research, promoting resource efficiency waste reduction. By combining environmental responsibility state-of-the-art functionality, redefining future 3D accelerating innovation regenerative medicine, drug development, fostering sustainable framework scientific discovery.

Language: Английский

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Engineering considerations in the design of tissue specific bioink for 3D Bioprinting applications

Biomaterials Science, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 23, 2024

Designing tissue-specific bioinks to replicate actual tissue environments and desired biomechanical properties.

Language: Английский

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