Large Fibrous Connective Tissue Reduces Oxidative Stress to Form a Living Cell Scaffold in Adipose Grafts

Antioxidants, Journal Year: 2025, Volume and Issue: 14(3), P. 270 - 270

Published: Feb. 26, 2025

This study aimed to investigate the mechanisms by which large fibrous connective (LFC) tissue enhances fat graft survival in transplantation. A block model demonstrated that intact containing LFC showed significantly higher rates compared with liposuctioned fat. In center of grafts, viable cells surrounded LFC, forming a mesh-like living structure. Proteomics extracellular matrix (ECM) adjacent (ALFC) and distant (DLFC) revealed significant differences mitochondrial aspects. Staining it contains number blood vessels mitochondria, exhibits stronger antioxidant capacity (p < 0.05) adipose tissue. By mixing transplanting into nude mice, histological sections promotes SOD1 expression, respiratory chain RNA reduces ROS inflammation. Pure mitochondrial-assisted transplantation only reduced short-term inflammation without improving long-term rates. conclusion, reducing oxidative stress grafts for cell survival, thereby overcoming distance limitations. represents novel mechanism distinct from classical models provides reference clinical practice.

Language: Английский

Lung dECM microcarriers for 3D proliferation of hUCMSCs and expression of growth factors

Journal of the American Ceramic Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 9, 2025

Abstract A simple 3D proliferation strategy for human umbilical cord‐derived mesenchymal stem cells (hUCMSCs) was developed based on porcine decellularized lung tissue microcarriers (DLTMs). The fixed with 75% ethanol, followed by decellularization and digestion pepsin‐acetic acid solution to form DLTMs an average diameter of 5–40 µm. presence key proteins, including collagen I, IV, laminin, fibronectin, elastin, confirmed through immunofluorescence staining, while the amount polysaccharides quantified. After centrifugation, hUCMSCs suspension formed spheroids which were cultured 14 days. Various techniques, DAPI, FDA H&E DNA quantification used assess performance hUCMSCs. Flow cytometric immunophenotyping employed, gene protein expressions cytokines growth factors such as IL‐10, HGF, KGF, Ang‐1 evaluated. results demonstrated that exhibited a porous structure simulated in vivo microenvironment, effectively enhancing immunomodulatory functions This showed potential integration bioreactor systems large‐scale production hUCMSC‐derived paracrine products therapeutic applications.

Language: Английский