Crosslinking Stabilization Strategy: A Novel Approach to Cartilage-like Repair of Annulus Fibrosus (AF) Defects DOI Creative Commons
Zihan Wang, Lei Wang,

Shaoshuo Li

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101625 - 101625

Published: March 5, 2025

Lumbar disc degeneration due to annulus fibrosus (AF) defects poses a significant challenge in clinical treatment Current treatments exhibit limited repair efficacy and high recurrence rate. To address this, we devised novel approach of crosslinking stabilization strategy. We integrated fibrinogen, thrombin, genipin, human bone marrow-derived mesenchymal stem cells (hBMSCs) hydrogel (FTGB) with acellular scaffold fascia (FTGB@S@F) remediate AF defects. FTIR analysis confirmed stable chemical within the FTGB hydrogel. demonstrated superior biocompatibility compared FB hydrogel, significantly higher cell viability (97.60 ± 2.02 % vs 81.43 4.50 %, P < 0.01) enhanced proliferation migration, as shown DAPI, Edu phalloidin staining. Atomic force microscopy (AFM) revealed that FTGB@S has dense reticular structure, enhancing material performance elastic modulus than FB@S. MTS testing showed FTGB@S@F outperformed other groups resisting cyclic axial load (25.53 1.17 MPa) maintaining height (0.57 0.12 mm), compression resistance minimal deformation. It also exhibited lowest rupture ROM (1.45 0.17 mm) close Intact control, demonstrating its potential restore mechanical function. MRI imaging group preserved an intact structure signal intensity, larger NP area (223.64 73.32 mm2 137.30 75.31 mm2, 0.05), (102.5 88.50 12.86 0.05). Histology reduced Un-repair FB@S@F groups. Transcriptomic identified upregulation PIGR downregulation COL4A3, linked PI3K-Akt pathway. Immunohistochemical qPCR analyses expression COL1, Aggrecan, RhoA, indicating effective regeneration.

Language: Английский

Crosslinking Stabilization Strategy: A Novel Approach to Cartilage-like Repair of Annulus Fibrosus (AF) Defects DOI Creative Commons
Zihan Wang, Lei Wang,

Shaoshuo Li

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101625 - 101625

Published: March 5, 2025

Lumbar disc degeneration due to annulus fibrosus (AF) defects poses a significant challenge in clinical treatment Current treatments exhibit limited repair efficacy and high recurrence rate. To address this, we devised novel approach of crosslinking stabilization strategy. We integrated fibrinogen, thrombin, genipin, human bone marrow-derived mesenchymal stem cells (hBMSCs) hydrogel (FTGB) with acellular scaffold fascia (FTGB@S@F) remediate AF defects. FTIR analysis confirmed stable chemical within the FTGB hydrogel. demonstrated superior biocompatibility compared FB hydrogel, significantly higher cell viability (97.60 ± 2.02 % vs 81.43 4.50 %, P < 0.01) enhanced proliferation migration, as shown DAPI, Edu phalloidin staining. Atomic force microscopy (AFM) revealed that FTGB@S has dense reticular structure, enhancing material performance elastic modulus than FB@S. MTS testing showed FTGB@S@F outperformed other groups resisting cyclic axial load (25.53 1.17 MPa) maintaining height (0.57 0.12 mm), compression resistance minimal deformation. It also exhibited lowest rupture ROM (1.45 0.17 mm) close Intact control, demonstrating its potential restore mechanical function. MRI imaging group preserved an intact structure signal intensity, larger NP area (223.64 73.32 mm2 137.30 75.31 mm2, 0.05), (102.5 88.50 12.86 0.05). Histology reduced Un-repair FB@S@F groups. Transcriptomic identified upregulation PIGR downregulation COL4A3, linked PI3K-Akt pathway. Immunohistochemical qPCR analyses expression COL1, Aggrecan, RhoA, indicating effective regeneration.

Language: Английский

Citations

0