Hyaluronic Acid-Coated Melt Electrowritten Scaffolds Promote Myoblast Attachment, Alignment, and Differentiation

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Abstract Purpose In muscle tissues, anisotropic cell alignment is essential for optimal fiber development and function. Biomaterials tissue engineering must guide cellular while supporting proliferation myogenic differentiation. Methods Here, we describe the fabrication of a tissue-engineered construct consisting scaffold aligned poly(ε-caprolactone) (PCL) microfibers coated in dynamic covalent hydrazone crosslinked hyaluronic acid (HA) hydrogel to support myoblast attachment, alignment, Norbornene modification HA further enabled functionalization with fibronectin-derived arginine-glycine-aspartic (RGD) peptide. Scaffolds were fabricated using melt electrowriting (MEW), three-dimensional (3D)-printing technique that uses stabilization fluid columns produce precisely polymeric microfibers. We evaluated scaffolds diameters 10 µm, 20 30 µm non-coated, HA-coated, HA-RGD-coated MEW through immunocytochemistry creatine kinase activity assays. Results HA-coated showed increased attachment C2C12 mouse skeletal myoblasts on all compared non-coated scaffolds, demonstrating highest attachment. All supported along fibers. Cells differentiated myotube formation as seen by myosin heavy chain (MHC) staining. Highest (CK) day 5 signified successful differentiation cells into mature myotubes. Conclusion This unique combination tunable biophysical biochemical cues enables creation biomimetic engineered scaffold, providing platform new therapeutic approaches regeneration.

Language: Английский

Moving Toward Biomimetic Tissue-Engineered Scaffolds

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(24), P. 2028 - 2028

Published: Dec. 17, 2024

Advancing experimental methodologies to accurately replicate the physiological and pathological characteristics of biological tissues is pivotal in tissue engineering [...]

Language: Английский