Induction of apoptosis and hypoxic stress in malignant melanoma cells via graphene-mediated far-infrared radiation DOI Creative Commons

Wumei Zhao,

Ziwen Chen, Wenxing Fu

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 7, 2025

Abstract Background Malignant melanoma (MM) is a highly aggressive skin tumor with rising incidence and poor prognosis. Although current clinical treatments, including surgery, targeted therapy, immunotherapy, radiotherapy, have shown some efficacy, therapeutic options remain limited for elderly patients those metastatic disease, highlighting the urgent need novel strategies. In recent years, unique far-infrared radiation (FIR) properties of graphene demonstrated potential applications in cancer treatment. However, mechanisms underlying FIR’s effects MM therapy poorly understood. Methods This study systematically evaluated inhibitory FIR on through vitro cell experiments, animal models, molecular mechanism analysis. First, B16F10 line was used as experimental model. The proliferation, apoptosis, cycle were assessed using CCK-8 assays flow cytometry, while RNA sequencing conducted to analyze associated signaling pathways. Second, specific caspase inhibitors employed further validate FIR-induced apoptosis. Finally, syngeneic transplantation model C57BL/6J mice established comfirm anti-tumor efficacy vivo, thereby comprehensively elucidating its anti-cancer mechanisms. Results results that significantly inhibits MM. experiments revealed treatment markedly suppressed induced caused G0/G1 phase arrest, downregulated expression hypoxia-related proteins such HIF-1α. studies, inhibited growth. exerts multiple Notably, use Z-DEVD-FMK Z-LEHD-FMK, which specifically inhibit caspase-3 caspase-9, respectively, can rescue cells from apoptosis by Conclusion elucidated mechanisms, inducing exacerbating hypoxic stress, causing arrest. findings provide new insights approaches establish theoretical foundation application therapy.

Language: Английский

Induction of apoptosis and hypoxic stress in malignant melanoma cells via graphene-mediated far-infrared radiation DOI Creative Commons

Wumei Zhao,

Ziwen Chen, Wenxing Fu

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 7, 2025

Abstract Background Malignant melanoma (MM) is a highly aggressive skin tumor with rising incidence and poor prognosis. Although current clinical treatments, including surgery, targeted therapy, immunotherapy, radiotherapy, have shown some efficacy, therapeutic options remain limited for elderly patients those metastatic disease, highlighting the urgent need novel strategies. In recent years, unique far-infrared radiation (FIR) properties of graphene demonstrated potential applications in cancer treatment. However, mechanisms underlying FIR’s effects MM therapy poorly understood. Methods This study systematically evaluated inhibitory FIR on through vitro cell experiments, animal models, molecular mechanism analysis. First, B16F10 line was used as experimental model. The proliferation, apoptosis, cycle were assessed using CCK-8 assays flow cytometry, while RNA sequencing conducted to analyze associated signaling pathways. Second, specific caspase inhibitors employed further validate FIR-induced apoptosis. Finally, syngeneic transplantation model C57BL/6J mice established comfirm anti-tumor efficacy vivo, thereby comprehensively elucidating its anti-cancer mechanisms. Results results that significantly inhibits MM. experiments revealed treatment markedly suppressed induced caused G0/G1 phase arrest, downregulated expression hypoxia-related proteins such HIF-1α. studies, inhibited growth. exerts multiple Notably, use Z-DEVD-FMK Z-LEHD-FMK, which specifically inhibit caspase-3 caspase-9, respectively, can rescue cells from apoptosis by Conclusion elucidated mechanisms, inducing exacerbating hypoxic stress, causing arrest. findings provide new insights approaches establish theoretical foundation application therapy.

Language: Английский

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