Self-assembled Antioxidant Enzyme-mimicking Hydrogel: Targeting Oxidative Stress and Macrophage Organization for Improving Degenerated Intervertebral Discs

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101586 - 101586

Published: Feb. 20, 2025

Intervertebral disc degeneration (IVDD) is a major contributor to lower back pain. At present, antioxidant therapy regarded as one of the most promising strategies for treating IVDD, due critical role reactive oxygen species (ROS) in its pathogenesis. Herein, we presented self-assembled hydrogel, termed MnGAHs, formed through crossing manganese ions (Mn2+) and glycyrrheic acid (GA), which possessed activities enzymes, including catalase (CAT) superoxide dismutase (SOD). The obtained MnGAHs effectively scavenge ROS, reducing oxidative stress levels alleviating senescence nucleus pulposus-derived mesenchymal stem cells (NPMSC), thereby mitigating IVDD. Furthermore, also promoted macrophage polarization towards M2 phenotype, inflammatory response inhibiting progression By combining theoretical calculations with analyses public databases, revealed that ROS-p53-p21 axis played crucial function reverse finding further confirmed by Western blot analysis. As result, injection into intervertebral (IVD) significantly alleviated process rat model puncture-induced Therefore, as-prepared enzyme-mimicking hydrogels provide effective approach

Language: Английский

Probiotic biofilm modified bioceramics for bone defect healing via osteogenesis, angiogenesis, and immune modulation

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: May 13, 2025

The failure to repair bone defects in a timely manner has detrimental effect on patients’ quality of life and functional status. Consequently, there are increasing demands for medical interventions promote healing defects. However, the local inflammation induced by implants side effects associated with systemic use drugs have prompted research into development bioactive materials. Recent reports indicated that oral administration Lactobacillus acidophilus (LA) can act as an immunomodulator. In this study, we strategically designed bioceramic scaffolds modified inactivated LA biofilms (LA@BC) through UV irradiation localized application LA. biosafety scaffold was validated at cellular animal levels ensure it be safely used without bacteraemia. LA@BC achieved M1 M2 polarization macrophages vitro reducing secretion inflammatory factors. addition, enhanced osteogenic marrow mesenchymal stem cells modulating Wnt/β-catenin signaling pathway. Furthermore, osteogenesis angiogenesis complement each other. exerted positive angiogenic endothelial cells. rat cranial defect model, upregulated expression RUNX2, OCN, CD31, IL-10 tissues, again demonstrating potent immunomodulatory effects. conclusion, provides new strategy clinical repair.

Language: Английский