Self-assembled Antioxidant Enzyme-mimicking Hydrogel: Targeting Oxidative Stress and Macrophage Organization for Improving Degenerated Intervertebral Discs

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101586 - 101586

Published: Feb. 20, 2025

Intervertebral disc degeneration (IVDD) is a major contributor to lower back pain. At present, antioxidant therapy regarded as one of the most promising strategies for treating IVDD, due critical role reactive oxygen species (ROS) in its pathogenesis. Herein, we presented self-assembled hydrogel, termed MnGAHs, formed through crossing manganese ions (Mn2+) and glycyrrheic acid (GA), which possessed activities enzymes, including catalase (CAT) superoxide dismutase (SOD). The obtained MnGAHs effectively scavenge ROS, reducing oxidative stress levels alleviating senescence nucleus pulposus-derived mesenchymal stem cells (NPMSC), thereby mitigating IVDD. Furthermore, also promoted macrophage polarization towards M2 phenotype, inflammatory response inhibiting progression By combining theoretical calculations with analyses public databases, revealed that ROS-p53-p21 axis played crucial function reverse finding further confirmed by Western blot analysis. As result, injection into intervertebral (IVD) significantly alleviated process rat model puncture-induced Therefore, as-prepared enzyme-mimicking hydrogels provide effective approach

Language: Английский

Colon-Targeted Mucoadhesive PLGA Microspheres Loaded with Ramulus Mori Alkaloids for Enhanced Water-Soluble Drug Delivery in Ulcerative Colitis Treatment

Molecules, Journal Year: 2025, Volume and Issue: 30(9), P. 1878 - 1878

Published: April 23, 2025

Ulcerative colitis (UC) is a chronic inflammation disease with severe impact on quality of life, limited treatment options. Ramulus Mori alkaloids (SZ-A) from Morus alba show promise for UC due to their safety and pharmacological effects, including anti-inflammation barrier repair. However, clinical use has been by gastrointestinal flatulence as side effect action an α-glucosidase inhibitor targeting the small intestine following oral administration. Therefore, constructing colon-targeted formulation deliver SZ-A advantageous strategy improve therapy. In this study, we used complex formed thiolated hyaluronic acid, which mucosal adhesion inflammation-targeting properties, intermediate carrier prepared sodium alginate-modified PLGA microspheres (SZ-A@MSs) double emulsion method achieve efficient encapsulation SZ-A. Specifically, alginate serves gastric acid protectant microbiota-responsive material, enabling precise responsive release in colonic region. SZ-A@MSs have particle size about 30 µm, drug loading 12.0%, efficiency 31.7% function through intestinal inflammatory sites. showed antioxidant anti-inflammatory abilities Raw264.7 cells. vivo imaging results suggest that good colon site retention sustained-release effect. Pharmacodynamic display efficacy, ability inhibit weight loss, atrophy, secretion factors. conclusion, colon-targeting can therapeutic provide potential UC.

Language: Английский