A Modular, Cost-Effective, and Pumpless Perfusion Assembly for the Long-Term Culture of Engineered Microvessels

Micromachines, Journal Year: 2025, Volume and Issue: 16(3), P. 351 - 351

Published: March 19, 2025

Continuous perfusion is necessary to sustain microphysiological systems and other microfluidic cell cultures. However, most of the established systems, such as syringe pumps, peristaltic rocker plates, have several operational challenges may be cost-prohibitive, especially for laboratories with no microsystems engineering expertise. Here, we address need a cost-efficient, easy-to-implement, reliable system. Our solution modular pumpless assembly (PPA), which constructed from commercially available, interchangeable, aseptically packaged syringes filters. The total cost components each assembled PPA USD 1–2. retains simplicity gravity-based flow but incorporates high resistance filters that enable slow sustained extended periods time (hours days). characteristics were determined by theoretical calculations hydraulic experimental characterization specific filter resistances. We demonstrated enabled long-term culture engineered endothelialized 3-D microvessels weeks. Taken together, our novel simply extremely low-cost available laboratory supplies facilitates robust compatibility current setups.

Language: Английский

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Surface behaviors of droplet manipulation in microfluidics devices

Advances in Colloid and Interface Science, Journal Year: 2022, Volume and Issue: 308, P. 102770 - 102770

Published: Sept. 7, 2022

Language: Английский

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Application of micro/nano-fluidics for encapsulation of food bioactive compounds - principles, applications, and challenges

Trends in Food Science & Technology, Journal Year: 2023, Volume and Issue: 136, P. 64 - 75

Published: April 1, 2023

Language: Английский

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Bioinspired Strong and Tough Organic–Inorganic Hybrid Fibers

Small Structures, Journal Year: 2023, Volume and Issue: 4(10)

Published: May 9, 2023

High‐strength and high‐toughness bio‐based fibers attract broad interest in biomechanical applications. Herein, strong tough organic–inorganic regenerated silk fibroin/hydroxyapatite (RSF/HAP) hybrid are prepared using a single‐channel microfluidic device. Calcium phosphate oligomers (CPOs) dispersed the RSF matrix first grow into spherical amorphous calcium phosphates (ACPs), which then crystallize needle‐like HAPs under humidity condition, mimicking biomineralization collagen bundles. better aligned along RSF/HAP fiber direction after poststretching, forming highly ordered densely packed microstructure within thus facilitating dense noncovalent interactions between rigid inorganic HAP nanocrystals flexible organic matrix. The endow with superior mechanical properties twisted bundles demonstrate remarkable tensile strength of 778 MPa, high Young's modulus 17.8 GPa, large strain 19.9%, an excellent toughness 121 MJ m −3 proper twisting treatments. also show good performances against static loading, dynamic impact, extreme cold condition they can maintain their down to −50 °C. Therefore, strategy is facile efficient.

Language: Английский

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Stimuli‐Triggered Multishape, Multimode, and Multistep Deformations Designed by Microfluidic 3D Droplet Printing

Small, Journal Year: 2023, Volume and Issue: 19(11)

Published: Jan. 15, 2023

Elastomers generally possess low Young's modulus and high failure strain, which are widely used in soft robots intelligent actuators. However, elastomers lack diverse functionalities, such as stimulated shape morphing, a general strategy to implement these functionalities into is still challenging. Here, microfluidic 3D droplet printing platform developed design composite architected with arrays of functional droplets. Functional droplets controlled size, composition, position, pattern designed implemented the elastomers, imparting performances systems. The composited sensitive stimuli, solvent, temperature, light, able demonstrate multishape (bow- S-shaped), multimode (gradual sudden), multistep (one- two-step) deformations. Based on unique properties droplet-embedded variety stimuli-responsive systems developed, including designable numbers, biomimetic flowers, robots, series achieved, presenting facile impart by printing.

Language: Английский

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Dynamically controllable hot spots in DNA-derived hydrogel scaffold SERS substrate for exosome recognition using DNA self-assembly amplification

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 496, P. 154270 - 154270

Published: July 24, 2024

Language: Английский

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SERS-based microfluidic sensor for sensitive detection of circulating tumor markers: A critical review

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 523, P. 216289 - 216289

Published: Oct. 23, 2024

Language: Английский

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Millifluidic-assisted ionic gelation technique for encapsulation of probiotics in double-layered polysaccharide structure

Food Research International, Journal Year: 2022, Volume and Issue: 160, P. 111699 - 111699

Published: July 18, 2022

Language: Английский

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Microfluidic-based technologies for diagnosis, prevention, and treatment of COVID-19: recent advances and future directions

Biomedical Microdevices, Journal Year: 2023, Volume and Issue: 25(2)

Published: March 13, 2023

Language: Английский

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Microfluidic fiber spinning for 3D bioprinting: Harnessing microchannels to build macrotissues

International Journal of Bioprinting, Journal Year: 2024, Volume and Issue: 10(1), P. 1404 - 1404

Published: Jan. 2, 2024

Microfluidics is rapidly revolutionizing the scientific panorama, providing unmatched high-throughput platforms that find application in numerous areas of physics, chemistry, biology, and materials science. Recently, microfluidic chips have been proposed, combination with bioactive materials, as promising tools for spinning cell-laden fibers on-demand characteristics. However, cells encapsulated filaments produced via technology are confined a quasi-three-dimensional (3D) environment fails to replicate intricate 3D architecture biological tissues. Thanks recent synergistic devices bioprinting technologies enable production sophisticated microfibers serving backbone structures, new age tissue engineering emerging. This review looks at how combining microfluidics printing contributing biofabrication relevant human substitutes implants. paper also describes whole manufacturing process from tool models, focusing on cutting-edge fabrication emphasizing most noticeable achievements technology. A theoretical insight thixotropic hydrogels proposed predict fiber size shear stress developing within channels. The potential using bio-printheads multi-material multi-cellular discussed, highlighting challenges still faces advancing field regenerative medicine purposes.

Language: Английский

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