Follicular fluid meiosis activating sterol supplementation enhances oocyte maturation and fertilization in a microfluidic system: A lab trial study DOI Creative Commons
Hossein Torkashvand, Ronak Shabani, Tayebe Artimani

et al.

International Journal of Reproductive BioMedicine (IJRM), Journal Year: 2024, Volume and Issue: 22(10), P. 781 - 792

Published: Dec. 6, 2024

Background: In vitro maturation (IVM) is a promising technique in assisted reproductive technologies, offering benefits such as reducing the risk of ovarian hyperstimulation syndrome. Objective: This study aimed to evaluate effects timed follicular fluid meiosis-activating sterol (FF-MAS) supplementation on IVM germinal vesicle oocytes using dynamic microfluidic system. Materials and Methods: this lab trial study, 266 were collected from Infertility Center Fatemieh hospital, Hamedan, Iran between June 2023 January 2024. The allocated into 3 groups for culture. Each group received culture medium at flow rate 0.36 μL/min 24 hr through inlet A FF-MAS B 1, 2, 6 hr. evaluated fertilization rates, embryo development, mitochondrial status, which was assessed JC-1 membrane potential assay. Results: Maturation rates significantly higher medium-term exposure (MTG) long-term compared short-term (STG) (p < 0.05). Fertilization also MTG STG Embryo formation proportion good-quality embryos (100% vs. 75%; p = 0.03) (83.3% 33.3%; 0.01), respectively. Mitochondrial peripheral distribution than 0.04). Conclusion: Optimizing duration enhances efficiency, strategy increase oocyte utilization in-vitro programs.

Language: Английский

Recent Advances in Nanotechnology-Based Drug Delivery Systems for the Diagnosis and Treatment of Reproductive Disorders DOI
Biswajeet Acharya, Amulyaratna Behera,

Suchismeeta Behera

et al.

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(3), P. 1336 - 1361

Published: Feb. 27, 2024

Over the past decade, nanotechnology has seen extensive integration into biomedical applications, playing a crucial role in biodetection, drug delivery, and diagnostic imaging. This is especially important reproductive health care, which become an emerging significant area of research. Global concerns have intensified around disorders such as infertility, endometriosis, ectopic pregnancy, erectile dysfunction, benign prostate hyperplasia, sexually transmitted infections, cancers. Nanotechnology presents promising solutions to address these by introducing innovative tools techniques, facilitating early detection, targeted improved imaging capabilities. Through utilization nanoscale materials devices, researchers can craft treatments that are not only more precise but also effective, significantly enhancing outcomes healthcare. Looking forward, future medicine holds immense potential for reshaping diagnostics, personalized therapies, fertility preservation. The nanotechnology-driven delivery systems anticipated elevate treatment effectiveness, minimize side effects, offer patients therapies efficient. review aims delve various types, properties, preparation techniques nanocarriers specifically designed context disorders, shedding light on current landscape directions this dynamic field.

Language: Английский

Citations

15
Advanced Sperm Selection Techniques for Assisted Reproduction DOI Open Access
Federica Cariati, Marina Orsi,

Francesca Bagnulo

et al.

Journal of Personalized Medicine, Journal Year: 2024, Volume and Issue: 14(7), P. 726 - 726

Published: July 4, 2024

Male infertility accounts for approximately 40% of cases. There are many causes male infertility, including environmental factors, age, lifestyle, infections, varicocele, and cancerous pathologies. Severe oligozoospermia, cryptozoospermia, azoospermia (obstructive non-obstructive) identified as severe factor once considered conditions sterility. Today, in vitro fertilization (IVF) techniques the only treatment strategy cases which new methodologies have been developed manipulation spermatozoa to achieve increase success rates. This review is an update techniques, particular sperm selection, emphasizing clinical case-specific methodology. The IVF process related diagnosis, appropriate choice treatment, effective preparation selection. In fact, selecting best guarantee optimal paternal heritage means increasing blastulation, implantation, ongoing pregnancy live birth rates, resulting greater techniques.

Language: Английский

Citations

4
Emerging Nanomedicine Approaches in Targeted Lung Cancer Treatment DOI Open Access

Isaic Alexandru,

Lavinia Davidescu, Alexandru Cătălin Motofelea

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11235 - 11235

Published: Oct. 19, 2024

Lung cancer, the leading cause of cancer-related deaths worldwide, is characterized by its aggressive nature and poor prognosis. As traditional chemotherapy has disadvantage non-specificity, nanomedicine offers innovative approaches for targeted therapy, particularly through development nanoparticles that can deliver therapeutic agents directly to cancer cells, minimizing systemic toxicity enhancing treatment efficacy. VEGF VEGFR are shown be responsible activating different signaling cascades, which will ultimately enhance tumor development, angiogenesis, metastasis. By inhibiting pathways, these nanotherapeutics effectively disrupt angiogenesis proliferation. This review highlights recent advancements in nanoparticle design, including lipid-based, polymeric, inorganic nanoparticles, their clinical implications improving lung outcomes, exploring role diagnoses treatment.

Language: Английский

Citations

2
The Role of the Vaginal and Endometrial Microbiomes in Infertility and Their Impact on Pregnancy Outcomes in Light of Recent Literature DOI Open Access

Bernadett Balla,

Anett Illés, Bálint Tóbiás

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 13227 - 13227

Published: Dec. 9, 2024

The Human Microbiome Project (HMP), initiated in 2007, aimed to gather comprehensive knowledge create a genetic and metabolic map of human-associated microorganisms their contribution physiological states predisposition certain diseases. Research has revealed that the human microbiome is highly diverse exhibits significant interpersonal variability; consequently, its exact impact on health remains unclear. With development next-generation sequencing (NGS) technologies, broad spectrum microbial communities been better characterized. lower female genital tract, particularly vagina, colonized by various bacterial species, with

Language: Английский

Citations

1
The regulation of intestinal flora on host’s genes may play an essential role in the development of endometrial hyperplastic processes in yang deficiency individuals DOI Open Access
Huixiang Zhang

International Journal of Clinical and Experimental Pathology, Journal Year: 2024, Volume and Issue: 17(4), P. 121 - 136

Published: Jan. 1, 2024

Yang-deficiency constitution (YADC) is linked to a higher vulnerability various diseases, such as cold coagulation and blood stasis (CCBS) syndrome infertility.Endometrial hyperplastic processes (EHPs) are leading cause of infertility in women characterized by CCBS.However, it remains unclear whether YADC related the development EHPs.Methods: We recruited 202 EHPs patients including 147 with (YEH group) 55 non-YADC (NYEH group).Fecal samples were collected from 8 YEH 3 NYEH analyzed using 16S rRNA V3-V4 sequencing for gut microbiota analysis.We obtained survey data differential dataset literature further analysis.Bioinformatics analysis was conducted microbiota-related genes public databases.Results: significantly more prevalent than (P < 0.001), suggesting potential risk factor occurrence (OR population = 13.471;OR healthy 5.173).The group had levels inflammation, estrogen, tamoxifen-related flora compared groups.There an interaction between flora, EHPs-related genes, particularly TNF gene (related inflammation) EGFR estrogen), which may play crucial role development.Conclusion: individuals exhibit significant changes their YADC.The specific host believed critical progression EHPs.

Language: Английский

Citations

0
Determination of Fertility Hormones and Adipokines by LC-MRM/MS Analysis DOI Creative Commons
Anna Illiano, Gabriella Pinto,

Amelia Mallardo

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(33), P. 35482 - 35489

Published: Aug. 8, 2024

Due to the increase in rate of male and female infertility, assisted fertilization practices are currently adopted as valid support for couples unable get pregnant. Analytical approaches fertility hormone dosages constantly being developed, following technological progress methods that have evolved more than a century. Indeed, analysis hormones serum samples is common clinical practice check state, but absolute quantification these great challenge due biological variability low concentrations. Currently, ELISA (enzyme-linked immunosorbent assay) based most used analytical techniques quantify blood settings. The current Article discusses development liquid chromatography-tandem mass spectrometry method (LC-MS/MS) monitor multiple protein nature single chromatographic run, i.e., LH (luteinizing hormone), FSH (follicle-stimulating TSH (thyroid-stimulating AMH (anti-Müllerian adiponectin, ghrelin, leptin, glucagon, obestatin. Particular attention has been paid hormone, whose ELISA-based known be controversial poor reproducibility between various kits used. For AMH, internal standard was quantitative determination compare data assays performed by an accredited laboratory on cohort from women aged 18 60 years. ability transitions LC-MRM/MS ensured both high specificity selectivity, which necessary steroid hormones, besides improvements reduced times costs.

Language: Английский

Citations

0
Nanoparticle-Mediated L-Carnitine Delivery for Improved Male Fertility DOI
A Saberi Moghadam,

Mohammad Javad Javid‐Naderi,

Sonia Fathi‐karkan

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 106420 - 106420

Published: Nov. 1, 2024

Language: Английский

Citations

0
Follicular fluid meiosis activating sterol supplementation enhances oocyte maturation and fertilization in a microfluidic system: A lab trial study DOI Creative Commons
Hossein Torkashvand, Ronak Shabani, Tayebe Artimani

et al.

International Journal of Reproductive BioMedicine (IJRM), Journal Year: 2024, Volume and Issue: 22(10), P. 781 - 792

Published: Dec. 6, 2024

Background: In vitro maturation (IVM) is a promising technique in assisted reproductive technologies, offering benefits such as reducing the risk of ovarian hyperstimulation syndrome. Objective: This study aimed to evaluate effects timed follicular fluid meiosis-activating sterol (FF-MAS) supplementation on IVM germinal vesicle oocytes using dynamic microfluidic system. Materials and Methods: this lab trial study, 266 were collected from Infertility Center Fatemieh hospital, Hamedan, Iran between June 2023 January 2024. The allocated into 3 groups for culture. Each group received culture medium at flow rate 0.36 μL/min 24 hr through inlet A FF-MAS B 1, 2, 6 hr. evaluated fertilization rates, embryo development, mitochondrial status, which was assessed JC-1 membrane potential assay. Results: Maturation rates significantly higher medium-term exposure (MTG) long-term compared short-term (STG) (p < 0.05). Fertilization also MTG STG Embryo formation proportion good-quality embryos (100% vs. 75%; p = 0.03) (83.3% 33.3%; 0.01), respectively. Mitochondrial peripheral distribution than 0.04). Conclusion: Optimizing duration enhances efficiency, strategy increase oocyte utilization in-vitro programs.

Language: Английский

Citations

0