DNA Binding, Nuclease/Photonuclease, and Phototoxicty Properties of Water Soluble Silicon (IV) Phthalocyanine DOI Open Access
Gökçe Seyhan, Ceren Boğuşlu, Can Özgür Yalçın

et al.

Farabi Tıp Dergisi, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Photodynamic therapy (PDT) is known as a method in which photosensitizers produce reactive oxygen species the presence of light and oxygen, leading to cell death. In this paper, DNA interaction properties bis[4-({8)-[3-(trimethylamino)phenoxy]octyl}oxy)] substituted silicon (IV) phthalocyanine (GsB-SiPc) were examined using UV-Vis spectrophometer agarose gel electrophoresis techniques. Afterwards, cytotoxic/phototoxic effects GsB-SiPc 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays on A549 cells. The results showed that bound ct-DNA via groove binding mode. nuclease/photonuclease experiments, had low nuclease activity dark but it high photonuclease light, depending compound concentration dose. addition, demonstrated remarkable phototoxicity toward human lung adenocarcinoma (A549) line at 50 100 µM light. vitro data revealed potential photodynamic agent for treatment cancer. These findings need be supported by further studies.

Language: Английский

DNA Binding, Nuclease/Photonuclease, and Phototoxicty Properties of Water Soluble Silicon (IV) Phthalocyanine DOI Open Access
Gökçe Seyhan, Ceren Boğuşlu, Can Özgür Yalçın

et al.

Farabi Tıp Dergisi, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Photodynamic therapy (PDT) is known as a method in which photosensitizers produce reactive oxygen species the presence of light and oxygen, leading to cell death. In this paper, DNA interaction properties bis[4-({8)-[3-(trimethylamino)phenoxy]octyl}oxy)] substituted silicon (IV) phthalocyanine (GsB-SiPc) were examined using UV-Vis spectrophometer agarose gel electrophoresis techniques. Afterwards, cytotoxic/phototoxic effects GsB-SiPc 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays on A549 cells. The results showed that bound ct-DNA via groove binding mode. nuclease/photonuclease experiments, had low nuclease activity dark but it high photonuclease light, depending compound concentration dose. addition, demonstrated remarkable phototoxicity toward human lung adenocarcinoma (A549) line at 50 100 µM light. vitro data revealed potential photodynamic agent for treatment cancer. These findings need be supported by further studies.

Language: Английский

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