Cyclosporine A Decreased Paclitaxel Resistance in Prostate Cancer Cells by Inhibiting MTDH Expression DOI Creative Commons
Jiangtao Li, Yuzhi Li, Xiaohong Zhang

et al.

American Journal of Men s Health, Journal Year: 2025, Volume and Issue: 19(1)

Published: Jan. 1, 2025

This study aims to investigate the effect and mechanism of cyclosporine A (CsA) on paclitaxel-resistant prostate cancer cells. Paclitaxel-resistant cell lines were established by gradual increment method. The proliferation cells was tested using MTT colony formation assay. Western blot used detect protein expression. Expression levels gene mRNA detected real-time polymerase chain reaction (RT-PCR). Xenografts in nude mice validate conclusion vitro. results showed that CsA could increase sensitivity paclitaxel. Treatment with gradients decreased metadherin (MTDH) RT-PCR decrease level MTDH. Overexpression MTDH increases paclitaxel resistance Conversely, knockdown reduced Treating failed reduce when overexpressed. yielded consistent conclusions vitro results. In conclusion, can vivo experiments have shown decreasing clinical practice, be combination improve therapeutic cancer. may serve as a novel target for treating

Language: Английский

Cyclosporine A Decreased Paclitaxel Resistance in Prostate Cancer Cells by Inhibiting MTDH Expression DOI Creative Commons
Jiangtao Li, Yuzhi Li, Xiaohong Zhang

et al.

American Journal of Men s Health, Journal Year: 2025, Volume and Issue: 19(1)

Published: Jan. 1, 2025

This study aims to investigate the effect and mechanism of cyclosporine A (CsA) on paclitaxel-resistant prostate cancer cells. Paclitaxel-resistant cell lines were established by gradual increment method. The proliferation cells was tested using MTT colony formation assay. Western blot used detect protein expression. Expression levels gene mRNA detected real-time polymerase chain reaction (RT-PCR). Xenografts in nude mice validate conclusion vitro. results showed that CsA could increase sensitivity paclitaxel. Treatment with gradients decreased metadherin (MTDH) RT-PCR decrease level MTDH. Overexpression MTDH increases paclitaxel resistance Conversely, knockdown reduced Treating failed reduce when overexpressed. yielded consistent conclusions vitro results. In conclusion, can vivo experiments have shown decreasing clinical practice, be combination improve therapeutic cancer. may serve as a novel target for treating

Language: Английский

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