Spatially Controlled MicroRNA Imaging in Mitochondria via Enzymatic Activation of Hybridization Chain Reaction DOI Open Access

Kaining Dai,

Jian Zhao, Lele Li

et al.

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 14, 2024

Abstract Live‐cell imaging of RNA in specific subcellular compartments is essential for elucidating the rich repertoire cellular functions, but it has been limited by a lack simple, precisely controlled methods. Here such an approach presented via combination hybridization chain reaction and spatially restricted enzymatic activation with organelle‐targeted delivery. The system can localize engineered DNA hairpins mitochondria, where target RNA‐initiated events selectively activated enzyme, enabling amplified high precision. It demonstrated that compatible live cell visualization enables regulatable microRNA mitochondria. Since situ signal amplification enzyme eliminates need genetically encoded protein overexpression, envisioned this simple platform will be broadly applicable precise resolution variety biological processes.

Language: Английский

Spatially Controlled MicroRNA Imaging in Mitochondria via Enzymatic Activation of Hybridization Chain Reaction DOI Open Access

Kaining Dai,

Jian Zhao, Lele Li

et al.

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 14, 2024

Abstract Live‐cell imaging of RNA in specific subcellular compartments is essential for elucidating the rich repertoire cellular functions, but it has been limited by a lack simple, precisely controlled methods. Here such an approach presented via combination hybridization chain reaction and spatially restricted enzymatic activation with organelle‐targeted delivery. The system can localize engineered DNA hairpins mitochondria, where target RNA‐initiated events selectively activated enzyme, enabling amplified high precision. It demonstrated that compatible live cell visualization enables regulatable microRNA mitochondria. Since situ signal amplification enzyme eliminates need genetically encoded protein overexpression, envisioned this simple platform will be broadly applicable precise resolution variety biological processes.

Language: Английский

Citations

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