lncRNA Biomarkers of Glioblastoma Multiforme

Biomedicines, Journal Year: 2024, Volume and Issue: 12(5), P. 932 - 932

Published: April 23, 2024

Long noncoding RNAs (lncRNAs) are RNA molecules of 200 nucleotides or more in length that not translated into proteins. Their expression is tissue-specific, with the vast majority involved regulation cellular processes and functions. Many human diseases, including cancer, have been shown to be associated deregulated lncRNAs, rendering them potential therapeutic targets biomarkers for differential diagnosis. The lncRNAs nervous system varies different cell types, implicated mechanisms neurons glia, effects on development functioning brain. Reports also a link between changes lncRNA etiopathogenesis brain neoplasia, glioblastoma multiforme (GBM). GBM an aggressive variant cancer unfavourable prognosis median survival 14-16 months. It considered brain-specific disease highly invasive malignant cells spreading throughout neural tissue, impeding complete resection, leading post-surgery recurrences, which prime cause mortality. early diagnosis could improve treatment extend survival, profiling biological fluids promising detection neoplastic at their initial stages effective interventions. This review presents systematic overview GBM-associated deregulation focus fingerprints patients' blood.

Language: Английский

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The pancancer overexpressed NFYC Antisense 1 controls cell cycle mitotic progression through in cis and in trans modes of action

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(3)

Published: March 11, 2024

Antisense RNAs (asRNAs) represent an underappreciated yet crucial layer of gene expression regulation. Generally thought to modulate their sense genes in cis through sequence complementarity or act transcription, asRNAs can also regulate different molecular targets trans, the nucleus cytoplasm. Here, we performed in-depth characterization NFYC 1 (NFYC-AS1), asRNA transcribed head-to-head subunit proliferation-associated NF-Y transcription factor. Our results show that NFYC-AS1 is a prevalently nuclear peaking early cell cycle. Comparative genomics suggests narrow phylogenetic distribution, with probable origin common ancestor mammalian lineages. overexpressed pancancer, preferentially association RB1 mutations. Knockdown by antisense oligonucleotides impairs growth lung squamous carcinoma and small cancer cells, phenotype recapitulated CRISPR/Cas9-deletion its start site. Surprisingly, affected only when endogenous manipulated. This regulation proliferation at least part independent transcription-mediated effect on possibly exerted RNA-dependent trans effects converging G2/M cycle phase genes. Accordingly, NFYC-AS1-depleted cells are stuck mitosis, indicating defects mitotic progression. Overall, emerged as cycle-regulating dual action, holding therapeutic potential types, including very aggressive RB1-mutated tumors.

Language: Английский

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The role of long noncoding RNAs in amyotrophic lateral sclerosis

Reviews in the Neurosciences, Journal Year: 2024, Volume and Issue: 35(5), P. 533 - 547

Published: March 7, 2024

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease with poor prognosis leading to death. The diagnosis and treatment of ALS are inherently challenging due its complex pathomechanism. Long noncoding RNAs (lncRNAs) transcripts longer than 200 nucleotides involved in different cellular processes, incisively gene expression. In recent years, more studies have been conducted on lncRNA classes interference pathologies, showing their promising contribution diagnosing treating diseases. this review, we discussed the role lncRNAs like NEAT1 C9orf72-as pathogenesis mechanisms caused by mutations genes, including TAR DNA-binding protein-43 (TDP-43), fused sarcoma (FUS), superoxide dismutase type 1 (SOD1). well-established pathogenesis; hence, elaborate involvement forming paraspeckles, stress response, inflammatory apoptosis. Furthermore, antisense (as-lncRNAs), key group from opposite strand ZEB1-AS1 ATXN2-AS, as newly identified components pathology ALS. Ultimately, review current standing using biomarkers therapeutic agents future vision further applications.

Language: Английский

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Targeting TDP-43 Proteinopathy in hiPSC-Derived Mutated hNPCs with Mitoxantrone Drugs and miRNAs

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(4), P. 410 - 410

Published: March 25, 2025

Background/Objectives: TDP-43 mutation-driven Amyotrophic Lateral Sclerosis (ALS) motor neuron disease is one of the most prominent forms (approximately 97%) in cases sporadic ALS. Dysfunctional autophagy and lysosomal function are prime mechanisms behind Mitoxantrone (Mito), a synthetic doxorubicin analog, an inhibitor DNA RNA synthesis/repair via intercalating with nitrogenous bases inhibiting topoisomerase II. The therapeutic potential miRNAs associated conditions has also been reported. This study explores Mito along against mutated protein-induced proteinopathy human-induced pluripotent stem cell (hiPSC)-derived human neural progenitor cells (hNPCs). Methods: HiPSCs for were differentiated into hNPCs used to explore at concentration 1 μM 24 h (the identified non-cytotoxic dose). effects on miRNA expression various cellular parameters such as mitochondrial dynamics, autophagy, stress granules assessed using high-throughput Open Array technique, immunocytochemistry, flow cytometry, immunoblotting, bioenergetic assay. Results: Mutated protein accumulation causes granule formation (G3BP1), dysfunction, SOD1 accumulation, hyperactivated ER hNPCs. show dysregulation six (miR-543, miR-34a, miR-200c, miR-22, miR-29b, miR-29c) A significant restoration mutation-induced alterations could be witnessed upon exposure Mito. Conclusions: Our indicates that miR-543, miR-34a have antisense alone combination Mitoxantrone.

Language: Английский

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The Emerging Role of Autophagy-Associated lncRNAs in the Pathogenesis of Neurodegenerative Diseases

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(11), P. 9686 - 9686

Published: June 2, 2023

Neurodegenerative diseases (NDDs) have become a significant global public health problem and major societal burden. The World Health Organization predicts that NDDs will overtake cancer as the second most common cause of human mortality within 20 years. Thus, it is urgently important to identify pathogenic diagnostic molecular markers related neurodegenerative processes. Autophagy powerful process for removing aggregate-prone proteins in neurons; defects autophagy are often associated with pathogenesis NDDs. Long non-coding RNAs (lncRNAs) been suggested key regulators neurodevelopment; aberrant regulation lncRNAs contributes neurological disorders. In this review, we summarize recent progress study context disorders, especially Alzheimer’s disease (AD) Parkinson’s (PD). information presented here should provide guidance future in-depth investigations processes treatment targets.

Language: Английский

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Inheritance of epigenetic transcriptional memory

Current Opinion in Genetics & Development, Journal Year: 2024, Volume and Issue: 85, P. 102174 - 102174

Published: March 1, 2024

Language: Английский

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Differentially expressed lncRNAs in SOD1 ^G93A mice skeletal muscle: H19, Myhas and Neat1 as potential biomarkers in amyotrophic lateral sclerosis

Open Biology, Journal Year: 2024, Volume and Issue: 14(10)

Published: Oct. 1, 2024

Amyotrophic lateral sclerosis (ALS) is a devastating neuromuscular disease characterized by progressive motor function and muscle mass loss. Despite extensive research in the field, underlying causes of ALS remain incompletely understood, contributing to absence specific diagnostic prognostic biomarkers effective therapies. This study investigates expression long-non-coding RNAs (lncRNAs) skeletal as potential source therapeutic targets for disease. The profiles 12 lncRNAs, selected from literature, were evaluated across different stages tissue biopsies SOD1 G93A transgenic mouse model ALS. Nine out lncRNAs differentially expressed, with Pvt1, H19 Neat1 showing notable increases symptomatic disease, suggesting their candidate support diagnosis key players pathophysiology Furthermore, progression Myhas RNA levels correlated longevity animal model, effectively discriminating between long- short-term survival individuals, thereby highlighting indicators. These findings underscore involvement especially Myhas, pathophysiology, offering novel insights diagnostic, targets.

Language: Английский

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Insights on ZEB1-AS1: emerging roles from cancer to neurodegeneration

Neural Regeneration Research, Journal Year: 2023, Volume and Issue: 19(6), P. 1187 - 1188

Published: Sept. 22, 2023

Carelli, Stephana*,#; Rey, Federica#; Maghraby, Erika#; Cereda, Cristina# Author Information

Language: Английский

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Study of lncRNAs in Pediatric Neurological Diseases: Methods, Analysis of the State-of-Art and Possible Therapeutic Implications

Pharmaceuticals, Journal Year: 2023, Volume and Issue: 16(11), P. 1616 - 1616

Published: Nov. 16, 2023

Long non-coding RNAs (lncRNAs) have emerged as crucial regulators in various cellular processes, and their roles pediatric neurological diseases are increasingly being explored. This review provides an overview of lncRNA implications the central nervous system, both its physiological state when a pathological condition is present. We describe role lncRNAs neural development, highlighting significance processes such stem cell proliferation, differentiation, synaptogenesis. Dysregulation specific associated with multiple diseases, neurodevelopmental or neurodegenerative disorders brain tumors. The collected evidence indicates that there need for further research to uncover full spectrum involvement While challenges exist, ongoing advancements technology our understanding biology offer hope future breakthroughs field neurology, leveraging potential therapeutic targets biomarkers.

Language: Английский

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