Extracellular Vesicle lncRNAs as Key Biomolecules for Cell-to-Cell Communication and Circulating Cancer Biomarkers DOI Creative Commons
Panagiotis Papoutsoglou, Antonin Morillon

Non-Coding RNA, Journal Year: 2024, Volume and Issue: 10(6), P. 54 - 54

Published: Nov. 5, 2024

Extracellular vesicles (EVs) are secreted by almost every cell type and considered carriers of active biomolecules, such as nucleic acids, proteins, lipids. Their content can be uptaken released into the cytoplasm recipient cells, thereby inducing gene reprogramming phenotypic changes in acceptor cells. Whether effects EVs on physiology cells mediated individual biomolecules or collective outcome total transferred EV is still under debate. The RNA consists several types RNA, messenger (mRNA), microRNA (miRNA), long non-coding (lncRNA), latter defined transcripts longer than 200 nucleotides that do not code for proteins but have important established biological functions. This review aims to update our insights functional roles their cargo during cancer progression, highlight utility novel diagnostic prognostic biomarkers cancer, tackle technological advances limitations identification, integrity assessment, preservation its functionality.

Language: Английский

Long non-coding RNAs in cancer: multifaceted roles and potential targets for immunotherapy DOI
Lokesh Kadian, Deepika Verma, Neelam Lohani

et al.

Molecular and Cellular Biochemistry, Journal Year: 2024, Volume and Issue: 479(12), P. 3229 - 3254

Published: Feb. 28, 2024

Language: Английский

Citations

9
From Diagnosis to Prognosis: The Transformative Impact of Exosomal ncRNAs in Head and Neck Cancer DOI Creative Commons
Aditi Patel, Kanisha Shah, Vivek Tanavde

et al.

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

This chapter explores the potential of exosome-derived non-coding RNAs (ncRNAs) as minimally invasive biomarkers for early diagnosis and prognosis head neck cancers (HNC). Late-stage diagnosis, lack disease-specific disease heterogeneity contribute significantly to poor survival rates HNC patients, highlighting urgent need novel biomarkers. The reviews emerging role ncRNAs in predicting onset HNC, with improved accurate risk assessment better prognosis. also offers insights into future ncRNA-based diagnostics personalized patient management aimed at improving 5-year outcomes reducing burden HNC.

Language: Английский

Citations

0
Decoding Salivary ncRNAomes as Novel Biomarkers for Oral Cancer Detection and Prognosis DOI Creative Commons
Subhadeep Das, Sandip Basak, Soumyadev Sarkar

et al.

Non-Coding RNA, Journal Year: 2025, Volume and Issue: 11(2), P. 28 - 28

Published: March 20, 2025

Oral cancer (OC) ranks among the most prevalent head and neck cancers, becoming eleventh common worldwide with ~350,000 new cases 177,000 fatalities annually. The rising trend in occurrence of OC young individuals women who do not have tobacco habits is escalating rapidly. Surgical procedures, radiation therapy, chemotherapy are treatment options for oral cancer. To achieve better therapy an early detection cancer, it essential to understand disease’s etiology at molecular level. Saliva, body fluid obtained non-invasively, holds a collection distinct non-coding RNA pools (ncRNAomes) that can be assessed as biomarkers identifying Non-coding signatures, which transcripts lacking protein-coding function, been identified significant progression various including This review aims examine role salivary ncRNAs (microRNA, circular RNA, lncRNA) associated disease explore their functions potential identification ensure survival outcomes patients.

Language: Английский

Citations

0
Novel insights on oral squamous cell carcinoma management using long non-coding RNAs DOI

Subhayan Sur,

Dimple Davray, Soumya Basu

et al.

Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics, Journal Year: 2024, Volume and Issue: 32(10), P. 1589 - 1612

Published: Jan. 1, 2024

Oral squamous cell carcinoma (OSCC) is one of the most prevalent forms head and neck carcinomas (HNSCC) with a poor overall survival rate (about 50%), particularly in cases metastasis. RNA-based cancer biomarkers are relatively advanced concept, non-coding RNAs currently have shown promising roles detection treatment various malignancies. This review underlines function long (lncRNAs) OSCC its subsequent clinical implications. LncRNAs, class RNAs, larger than 200 nucleotides resemble mRNA numerous ways. However, unlike mRNA, lncRNA regulates multiple druggable non-druggable signaling molecules through simultaneous interaction DNA, RNA, proteins, or microRNAs depending on concentration localization cells. Upregulation oncogenic lncRNAs down-regulation tumor suppressor evident tissues body fluids such as blood saliva indicating their potential valuable biomarkers. Targeted inhibition candidate over-expression showed therapeutic

Language: Английский

Citations

3
Characterization of non-invasive oropharyngeal samples and nucleic acid isolation for molecular diagnostics DOI Creative Commons

Leonie Hose,

Matthias Schürmann,

Inga Mennebröcker

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 19, 2024

Abstract Molecular diagnostics is an increasingly important clinical tool, especially in routine sampling. We evaluated two non-invasive methods (oral swabs and mouthwashes) for sampling nucleic acids from the oral/pharyngeal area. created a workflow sample collection (n = 59) to RT-qPCR based analysis. The samples were further characterized terms of their cellular composition as well purity, degradation microbial content derived DNA/RNA. determined optimal housekeeping genes applicable these types samples. indicated that mouthwashes contained more immune cells bacteria. Even though protocol was not specifically optimized extract bacterial RNA it possible derive RNA, both methods. Optimizing allowed us generate stable quantities DNA/RNA purity parameters significantly different between integrity analysis demonstrated high level corresponding confirmed sequencing potential. TATA-Box Binding Protein most favorable gene. In summary, we have developed robust method suitable multiple downstream diagnostic techniques. This can be used foundation research endeavors focusing on developing molecular oropharyngeal cavity.

Language: Английский

Citations

2
Non-Coding RNAs in Oral Cancer: Emerging Biomarkers and Therapeutic Frontier DOI Creative Commons
Mehrdad Hashemi,

Saloomeh Khoushab,

Mina Hobabi Aghmiuni

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(21), P. e40096 - e40096

Published: Nov. 1, 2024

Around the world, oral cancer (OC) is a major public health problem, resulting in significant number of deaths each year. Early detection and treatment are crucial for improving patient outcomes. Recent progress DNA sequencing transcriptome profiling has revealed extensive non-coding RNAs (ncRNAs) transcription, underscoring their regulatory importance. NcRNAs influence genomic transcription translation molecular signaling pathways, making them valuable various clinical applications. Combining spatial transcriptomics (ST) metabolomics (SM) with single-cell RNA provides deeper insights into tumor microenvironments, enhancing diagnostic therapeutic precision OC. Additionally, exploration salivary biomarkers offers non-invasive avenue. This article explores potential ncRNAs as tools

Language: Английский

Citations

1
Epigenetic modulation of long noncoding RNA H19 in oral squamous cell carcinoma-A narrative review DOI Creative Commons
Peramaiyan Rajendran, Ramya Sekar, Basem M. Abdallah

et al.

Non-coding RNA Research, Journal Year: 2024, Volume and Issue: 9(2), P. 602 - 611

Published: Feb. 1, 2024

Oral squamous cell carcinoma (OSCC) showed a seemingly increasing incidence in the last decade. In India, despite use of tobacco decreased rapidly, past five years, pattern OSCC over gender and age drastic shift. About 51 % head neck cancers are not associated with habits. Studies exploring various contributing factors this malignancy have documented. Recently, epigenetic induction progression were explored. More than 90 human genome is made up non-coding transcriptome, which believed to be noises. However, these RNAs identified major modulators, raises concern non-habit patients. H19 long non coding RNA proved an effective biomarker carcinoma. Its role oral cancer was investigated depth. This review discusses detail inducing carcinogenesis.

Language: Английский

Citations

0
Silencing Exosomal circ102927 Inhibits Foot Melanoma Metastasis via Regulating Invasiveness, Epithelial-Mesenchymal Transition and Apoptosis DOI Creative Commons

Huiying Wan,

Ling Zhong, Tian Xia

et al.

Cancer Management and Research, Journal Year: 2024, Volume and Issue: Volume 16, P. 825 - 839

Published: July 1, 2024

Background: Exosomes contain abundant circular RNAs (circRNAs), playing an important role in intercellular communication. However, the function and underlying molecular mechanism of exosomal circRNAs foot metastatic melanoma remain unclear. Methods: Twelve differentially expressed between patients with primary were screened through high-throughput sequencing, their expression levels detected by real-time reverse transcriptase-polymerase chain reaction (RT-qPCR). CircRNA102927 silencing overexpression A2058 cell line was constructed, effects circRNA102927 on proliferation, apoptosis, migration, invasion, epithelial-mesenchymal transition (EMT) assessed using counting kit-8 (CCK-8), flow cytometry, wound healing, Transwell, Western blot assays, respectively. Results: ROC curve showed that six could be used as diagnostic biomarkers for melanoma. Melanoma-secreted exosomes induced differentiation CD4+ T cells into Treg cells. highly melanomas. Functionally, inhibited EMT, invasion cells, while promoting apoptosis. Meanwhile, had opposite effects. Conclusion: Our investigation suggests may suppress metastasis inhibiting invasiveness, EMT Keywords: circRNAs, CircRNA102927, melanoma,

Language: Английский

Citations

0
Extracellular Vesicle lncRNAs as Key Biomolecules for Cell-to-Cell Communication and Circulating Cancer Biomarkers DOI Creative Commons
Panagiotis Papoutsoglou, Antonin Morillon

Non-Coding RNA, Journal Year: 2024, Volume and Issue: 10(6), P. 54 - 54

Published: Nov. 5, 2024

Extracellular vesicles (EVs) are secreted by almost every cell type and considered carriers of active biomolecules, such as nucleic acids, proteins, lipids. Their content can be uptaken released into the cytoplasm recipient cells, thereby inducing gene reprogramming phenotypic changes in acceptor cells. Whether effects EVs on physiology cells mediated individual biomolecules or collective outcome total transferred EV is still under debate. The RNA consists several types RNA, messenger (mRNA), microRNA (miRNA), long non-coding (lncRNA), latter defined transcripts longer than 200 nucleotides that do not code for proteins but have important established biological functions. This review aims to update our insights functional roles their cargo during cancer progression, highlight utility novel diagnostic prognostic biomarkers cancer, tackle technological advances limitations identification, integrity assessment, preservation its functionality.

Language: Английский

Citations

0