A modified system to promote stemness of mouse intestinal stem cells by activating Nrf2 and α2-adrenergic receptor signaling pathway DOI Creative Commons
Xingyu Zhou, Yang Li,

Sentao Song

et al.

Acta Biochimica et Biophysica Sinica, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Intestinal stem cells (ISCs) maintain epithelial homeostasis through continuous self-renewal and differentiation, but their regulatory mechanisms remain incompletely understood. Using a simplified culture system, we identify two novel pathways that synergistically enhance cell characteristics: antioxidant signaling 2-phospho-L-ascorbic acid (pVc) α2-adrenergic receptor (α2-AR) activation by dexmedetomidine (Dex). Mechanistic studies reveal pVc promotes maintenance Nrf2-mediated responses, while α2-AR functions suppression of cAMP signaling. In vivo administration these compounds enhances intestinal renewal maintaining proper positioning identity. Notably, regeneration after radiation injury enhancing proliferation produced Bmi1 + in the post-injury process, demonstrating therapeutic potential. These findings advance our understanding ISC regulation suggest new strategies for protecting integrity during or disease.

Language: Английский

A modified system to promote stemness of mouse intestinal stem cells by activating Nrf2 and α2-adrenergic receptor signaling pathway DOI Creative Commons
Xingyu Zhou, Yang Li,

Sentao Song

et al.

Acta Biochimica et Biophysica Sinica, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Intestinal stem cells (ISCs) maintain epithelial homeostasis through continuous self-renewal and differentiation, but their regulatory mechanisms remain incompletely understood. Using a simplified culture system, we identify two novel pathways that synergistically enhance cell characteristics: antioxidant signaling 2-phospho-L-ascorbic acid (pVc) α2-adrenergic receptor (α2-AR) activation by dexmedetomidine (Dex). Mechanistic studies reveal pVc promotes maintenance Nrf2-mediated responses, while α2-AR functions suppression of cAMP signaling. In vivo administration these compounds enhances intestinal renewal maintaining proper positioning identity. Notably, regeneration after radiation injury enhancing proliferation produced Bmi1 + in the post-injury process, demonstrating therapeutic potential. These findings advance our understanding ISC regulation suggest new strategies for protecting integrity during or disease.

Language: Английский

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