Impact of a Withania somnifera and Bacopa monnieri Formulation on SH-SY5Y Human Neuroblastoma Cells Metabolism Through NMR Metabolomic DOI Open Access

Maria D’Elia,

Carmen Marino,

Rita Celano

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(23), P. 4096 - 4096

Published: Nov. 28, 2024

Objectives: This study investigates the effectiveness of an herbal formulation, STRESSLESS (ST-65), which combines ashwagandha (Withania somnifera) and bacopa (Bacopa monnieri), on SH-SY5Y human neuroblastoma cells. Given rising interest in natural compounds for neuroprotection stress alleviation, we aimed to explore cellular molecular effects this formulation. Methods: Utilizing a nuclear magnetic resonance (NMR) metabolomic approach ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS), identified key bioactive ST-65, including withanolides from bacosides bacopa. Results: Our findings indicate that ST-65 treatment significantly alters metabolic profile Key changes included increased levels metabolites linked neuroprotection, energy metabolism, antioxidant defense. Notable enhancements were observed specific amino acids neuroprotective compounds, suggesting activation mechanisms mitigation stress-induced damage. Conclusions: The reveals complex phyto-chemical underscores its potential as active agent addressing stress-related neurodegenerative conditions. These insights into neuronal provide foundation further exploration formulations neuroprotection.

Language: Английский

Neuro-nutraceuticals: Emerging molecular and functional insights into how natural products improve brain health DOI
Kochupurackal P. Mohanakumar, Catarina Rendeiro, Philip M. Beart

et al.

Neurochemistry International, Journal Year: 2025, Volume and Issue: unknown, P. 105948 - 105948

Published: Feb. 1, 2025

Language: Английский

Citations

0
Impact of a Withania somnifera and Bacopa monnieri Formulation on SH-SY5Y Human Neuroblastoma Cells Metabolism Through NMR Metabolomic DOI Open Access

Maria D’Elia,

Carmen Marino,

Rita Celano

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(23), P. 4096 - 4096

Published: Nov. 28, 2024

Objectives: This study investigates the effectiveness of an herbal formulation, STRESSLESS (ST-65), which combines ashwagandha (Withania somnifera) and bacopa (Bacopa monnieri), on SH-SY5Y human neuroblastoma cells. Given rising interest in natural compounds for neuroprotection stress alleviation, we aimed to explore cellular molecular effects this formulation. Methods: Utilizing a nuclear magnetic resonance (NMR) metabolomic approach ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS), identified key bioactive ST-65, including withanolides from bacosides bacopa. Results: Our findings indicate that ST-65 treatment significantly alters metabolic profile Key changes included increased levels metabolites linked neuroprotection, energy metabolism, antioxidant defense. Notable enhancements were observed specific amino acids neuroprotective compounds, suggesting activation mechanisms mitigation stress-induced damage. Conclusions: The reveals complex phyto-chemical underscores its potential as active agent addressing stress-related neurodegenerative conditions. These insights into neuronal provide foundation further exploration formulations neuroprotection.

Language: Английский

Citations

0