Lagging Brain Gene Expression Patterns of Drosophila melanogaster Young Adult Males Confound Comparisons Between Sexes DOI Creative Commons
Flannery McLamb, Zuying Feng, Jeanne P. Vu

et al.

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 28, 2024

Abstract Many species, including fruit flies ( Drosophila melanogaster ), are sexually dimorphic. Phenotypic variation in morphology, physiology, and behavior can affect development, reproduction, health, aging. Therefore, designating sex as a variable sex-blocking should be considered when designing experiments. The brain regulates phenotypes throughout the lifespan by balancing survival sex-specific development at each life stage is likely. Changes morphology physiology governed differential gene expression, quantifiable molecular marker for age- variations. We assessed fly transcriptome three adult ages expression signatures of sex, age, sex-by-age: 6698 genes were differentially expressed between sexes, with most divergence 3 days. Between ages, 31.1% 6084 (1890 genes) share similar patterns from to 7 days females, 14 males. Most these (90.5%, 1712) upregulated enriched chemical stimulus detection and/or cilium regulation. Our data highlight an important delay male regulation compared females. Because significant delays could confound comparisons studies sexual dimorphism phenotypically comparable stages rather than chronological age more biologically relevant.

Language: Английский

Differential Developmental Neurotoxicity and Tissue Uptake of the Per- and Polyfluoroalkyl Substance Alternatives, GenX and PFBS DOI

Ola Wasel,

Hanna King, Youn Jeong Choi

et al.

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(48), P. 19274 - 19284

Published: Nov. 9, 2023

Per- and polyfluoroalkyl substances (PFAS) are a class of synthetic chemicals with several applications. Multiple adverse health effects reported for longer carbon chain (≤C8) PFAS. Shorter PFAS, [e.g., hexafluoropropylene oxide dimer acid (HFPO–DA; GenX) perfluorobutanesulfonic (PFBS)] were introduced as alternatives. Past studies indicate that longer-chain PFAS neurotoxic targeting the dopamine pathway, but it is not known if shorter-chain act similarly. This study aimed to evaluate developmental neurotoxicity tissue uptake GenX PFBS using zebrafish (Danio rerio). First, acute toxicity was assessed by measuring LC50 at 120 h postfertilization (hpf). Body burden determined after embryonic exposure (1–72 hpf) sublethal concentrations or LC-ESI-MS/MS. Locomotor activity visual motor response assay hpf levels 72 exposure. more acutely toxic bioaccumulative than GenX. caused hyperactivity hpf, stronger behavioral alterations observed PFBS. An increase in whole organism occurred 40 ppb GenX, while decrease 400 Differences detected these two differential mechanisms neurotoxicity.

Language: Английский

Citations

26
Environmentally relevant concentrations of individual per- and polyfluoroalkyl substances (PFAS) and a PFAS mixture impact proliferation, migration, and gene transcription in a human myometrial cell line. DOI
Kendra L Clark, Jitu W. George

Toxicology, Journal Year: 2025, Volume and Issue: 515, P. 154173 - 154173

Published: May 5, 2025

Language: Английский

Citations

0
Emerging Perfluorinated Chemical GenX: Environmental and Biological Fates and Risks DOI Creative Commons

Wan-Qian Guo,

Weidong Hao,

Wusheng Xiao

et al.

Environment & Health, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Perfluorinated chemical GenX, formally known as hexafluoropropylene oxide dimer acid (HFPO–DA), has been applied an alternative to the forever perfluorooctanoic (PFOA). The applications of HFPO–DA have rapidly expanded from traditional nonstick coating industries into high-tech semiconductor manufacturing. Because such facts in conjunction with its low biodegradation rate and high potential long-distance atmospheric transport, presence accumulation ubiquitously detected environmental media biological species, including animals human beings, posing alarming urgent needs for risk assessment HFPO–DA. Building on United States Environmental Protection Agency's evaluation 2021, this review first summarizes interaction environment, elaborates toxicities carcinogenicity, along their possible mechanisms, briefly addresses current exposure management strategies. These lines evidence support that safety necessitates further investigation monitoring, albeit being considered a less toxic persistence substitute PFOA.

Language: Английский

Citations

2
Reproductive toxicities of dimethyl phthalates on Caenorhabditis elegans with alteration in responses over life stages DOI
Jing Zhang, Yuheng Huang, Zhenyang Yu

et al.

Land Degradation and Development, Journal Year: 2024, Volume and Issue: 35(7), P. 2635 - 2642

Published: March 21, 2024

Abstract Phthalate esters (PAEs) raised serious concerns on their health risk due to toxicities. However, toxicities of low‐molecular‐weight PAEs, for example, dimethyl phthalates (DMPs), remained poorly investigated. In the present study, reproductive (o‐)phthalate (DMOP), (p‐)phthalate (DMPP), and (m‐)phthalate (DMMP) were measured Caenorhabditis elegans with mechanical exploration. Results showed that DMPP, DMOP, DMMP commonly inhibited total reproduction while stimulated lifespan, showing trade‐off effects. effects gene expressions, caused different regulations expressions vab‐1 , ceh‐18 gsa‐1 which are involved in oocyte growth, ovulation, maturation. Meanwhile, they downregulated those rme‐2 rcy‐4 regulate germ cells gonads lipid metabolism oocytes. Moreover, biochemicals processes alteration between stimulation inhibition over life stages. addition, at apical, biochemical, molecular levels clearly structure influences Summing up, DMPs depended exposure stages also chemical structures.

Language: Английский

Citations

1
Comparative Proteomics Highlights that GenX Exposure Leads to Metabolic Defects and Inflammation in Astrocytes DOI Creative Commons

Abdulla Abu-Salah,

Müberra Fatma Cesur, Aiesha Anchan

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(46), P. 20525 - 20539

Published: Nov. 5, 2024

Exposure to PFAS such as GenX (HFPO dimer acid) has become increasingly common due the replacement of older generation in manufacturing processes. While neurodegenerative and developmental effects legacy exposure have been studied depth, there is a limited understanding specific exposure. To investigate exposure, we exposed Drosophila melanogaster assessed motor behavior performed quantitative proteomics fly brains identify molecular changes brain. Additionally, metabolic network-based analysis using iDrosophila1 model unveiled potential link between neurodegeneration. Since linked Parkinson's disease (PD), compared proteome data sets GenX-exposed flies PD expressing human α-synuclein. Considering proteomic data- analyses that revealed may be regulating GABA-associated pathways immune system, next explored on astrocytes, astrocytes brain can regulate GABA. An array assays demonstrated lead mitochondrial dysfunction neuroinflammatory response possibly linking non-cell autonomous neurodegeneration deficits associated with

Language: Английский

Citations

1
Lagging Brain Gene Expression Patterns of Drosophila melanogaster Young Adult Males Confound Comparisons Between Sexes DOI Creative Commons
Flannery McLamb, Zuying Feng, Jeanne P. Vu

et al.

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 28, 2024

Abstract Many species, including fruit flies ( Drosophila melanogaster ), are sexually dimorphic. Phenotypic variation in morphology, physiology, and behavior can affect development, reproduction, health, aging. Therefore, designating sex as a variable sex-blocking should be considered when designing experiments. The brain regulates phenotypes throughout the lifespan by balancing survival sex-specific development at each life stage is likely. Changes morphology physiology governed differential gene expression, quantifiable molecular marker for age- variations. We assessed fly transcriptome three adult ages expression signatures of sex, age, sex-by-age: 6698 genes were differentially expressed between sexes, with most divergence 3 days. Between ages, 31.1% 6084 (1890 genes) share similar patterns from to 7 days females, 14 males. Most these (90.5%, 1712) upregulated enriched chemical stimulus detection and/or cilium regulation. Our data highlight an important delay male regulation compared females. Because significant delays could confound comparisons studies sexual dimorphism phenotypically comparable stages rather than chronological age more biologically relevant.

Language: Английский

Citations

0