Neuron,
Journal Year:
2023,
Volume and Issue:
111(22), P. 3570 - 3589.e5
Published: Nov. 1, 2023
Efforts
are
ongoing
to
map
synaptic
wiring
diagrams,
or
connectomes,
understand
the
neural
basis
of
brain
function.
However,
chemical
synapses
represent
only
one
type
functionally
important
neuronal
connection;
in
particular,
extrasynaptic,
"wireless"
signaling
by
neuropeptides
is
widespread
and
plays
essential
roles
all
nervous
systems.
By
integrating
single-cell
anatomical
gene-expression
datasets
with
biochemical
analysis
receptor-ligand
interactions,
we
have
generated
a
draft
connectome
neuropeptide
C.
elegans
system.
This
network
characterized
high
connection
density,
extended
cascades,
autocrine
foci,
decentralized
topology,
large,
highly
interconnected
core
containing
three
constituent
communities
sharing
similar
patterns
input
connectivity.
Intriguingly,
several
key
hubs
little-studied
neurons
that
appear
specialized
for
peptidergic
neuromodulation.
We
anticipate
neuropeptidergic
will
serve
as
prototype
how
networks
neuromodulatory
organized.
Nature,
Journal Year:
2023,
Volume and Issue:
624(7991), P. 317 - 332
Published: Dec. 13, 2023
The
mammalian
brain
consists
of
millions
to
billions
cells
that
are
organized
into
many
cell
types
with
specific
spatial
distribution
patterns
and
structural
functional
properties1-3.
Here
we
report
a
comprehensive
high-resolution
transcriptomic
cell-type
atlas
for
the
whole
adult
mouse
brain.
was
created
by
combining
single-cell
RNA-sequencing
(scRNA-seq)
dataset
around
7
million
profiled
(approximately
4.0
passing
quality
control),
approximately
4.3
using
multiplexed
error-robust
fluorescence
in
situ
hybridization
(MERFISH).
is
hierarchically
4
nested
levels
classification:
34
classes,
338
subclasses,
1,201
supertypes
5,322
clusters.
We
present
an
online
platform,
Allen
Brain
Cell
Atlas,
visualize
whole-brain
along
MERFISH
datasets.
systematically
analysed
neuronal
non-neuronal
across
identified
high
degree
correspondence
between
identity
specificity
each
type.
results
reveal
unique
features
organization
different
regions-in
particular,
dichotomy
dorsal
ventral
parts
part
contains
relatively
fewer
yet
highly
divergent
types,
whereas
more
numerous
closely
related
other.
Our
study
also
uncovered
extraordinary
diversity
heterogeneity
neurotransmitter
neuropeptide
expression
co-expression
types.
Finally,
found
transcription
factors
major
determinants
classification
combinatorial
factor
code
defines
all
establishes
benchmark
reference
foundational
resource
integrative
investigations
cellular
circuit
function,
development
evolution
