Oscillatory brain activity in spontaneous and induced sleep stages in flies DOI Creative Commons
Melvyn Yap, Martyna Grabowska, Chelsie Rohrscheib

et al.

Nature Communications, Journal Year: 2017, Volume and Issue: 8(1)

Published: Nov. 22, 2017

Sleep is a dynamic process comprising multiple stages, each associated with distinct electrophysiological properties and potentially serving different functions. While these phenomena are well described in vertebrates, it unclear if invertebrates have sleep stages. We perform local field potential (LFP) recordings on flies spontaneously sleeping, compare their brain activity to induced using either genetic activation of sleep-promoting circuitry or the GABAA agonist Gaboxadol. find transitional stage 7-10 Hz oscillation central during spontaneous sleep. Oscillatory also evident when we acutely activate neurons dorsal fan-shaped body (dFB) Drosophila. In contrast, following Gaboxadol exposure characterized by low-amplitude LFPs, which dFB-induced effects suppressed. thus appears involve at least two stages: increased oscillatory activity, particularly induction, followed desynchronized decreased activity.

Language: Английский

Identification of Neurons with a Privileged Role in Sleep Homeostasis in Drosophila melanogaster DOI Creative Commons

Glen Seidner,

James E. Robinson, Mei‐Lin Wu

et al.

Current Biology, Journal Year: 2015, Volume and Issue: 25(22), P. 2928 - 2938

Published: Nov. 1, 2015

Language: Английский

Citations

138
Allatostatin A Signalling in Drosophila Regulates Feeding and Sleep and Is Modulated by PDF DOI Creative Commons
Jiangtian Chen,

Wencke Reiher,

Christiane Hermann-Luibl

et al.

PLoS Genetics, Journal Year: 2016, Volume and Issue: 12(9), P. e1006346 - e1006346

Published: Sept. 30, 2016

Feeding and sleep are fundamental behaviours with significant interconnections cross-modulations. The circadian system peptidergic signals important components of this modulation, but still little is known about the mechanisms networks by which they interact to regulate feeding sleep. We show that specific thermogenetic activation Allatostatin A (AstA)-expressing PLP neurons enteroendocrine cells reduces promotes in fruit fly Drosophila. effects AstA cell mediated peptides receptors homolog galanin subserving similar apparently conserved functions vertebrates. further identify as a downstream target neuropeptide pigment-dispersing factor (PDF), an output clock. contacted PDF-expressing clock neurons, express functional PDF receptor demonstrated cAMP imaging. Silencing signalling continuous input tethered changes sleep/activity ratio opposite directions does not affect rhythmicity. Taken together, our results suggest pleiotropic distinct neuronal subset adapts digestive energy-saving state can be modulated PDF.

Language: Английский

Citations

138
Molecular Mechanisms of Sleep Homeostasis in Flies and Mammals DOI Open Access
Ravi Allada, Chiara Cirelli, Amita Sehgal

et al.

Cold Spring Harbor Perspectives in Biology, Journal Year: 2017, Volume and Issue: 9(8), P. a027730 - a027730

Published: April 21, 2017

Ravi Allada1, Chiara Cirelli2 and Amita Sehgal3 1Department of Neurobiology, Northwestern University, Evanston, Ilinois 60208 2Department Psychiatry, University Wisconsin-Madison, Madison, Wisconsin 53719 3Department Neuroscience, Perelman School Medicine at Pennsylvania, Philadelphia, Pennsylvania 19104-6058 Correspondence: r-allada{at}northwestern.edu; ccirelli{at}wisc.edu; amita{at}mail.med.upenn.edu

Language: Английский

Citations

138
Drosophila Neuropeptide F Signaling Independently Regulates Feeding and Sleep-Wake Behavior DOI Creative Commons
Brian Y. Chung, Jennifer Ro,

Sabine A. Hutter

et al.

Cell Reports, Journal Year: 2017, Volume and Issue: 19(12), P. 2441 - 2450

Published: June 1, 2017

Proper regulation of sleep-wake behavior and feeding is essential for organismal health survival. While previous studies have isolated discrete neural loci substrates important either sleep or feeding, how the brain organized to coordinate both processes with respect one another remains poorly understood. Here, we provide evidence that Drosophila Neuropeptide F (NPF) network forms a critical component adult regulation. Activation NPF signaling in promotes wakefulness likely through its cognate receptor NPFR. Flies carrying loss-of-function allele do not suppress following prolonged starvation conditions, suggesting acts as hunger signal keep animal awake. NPF-expressing cells, specifically those expressing circadian photoreceptor cryptochrome, are largely responsible changes caused by neuron activation, but demonstrating different neurons separately drive hunger.

Language: Английский

Citations

128
Oscillatory brain activity in spontaneous and induced sleep stages in flies DOI Creative Commons
Melvyn Yap, Martyna Grabowska, Chelsie Rohrscheib

et al.

Nature Communications, Journal Year: 2017, Volume and Issue: 8(1)

Published: Nov. 22, 2017

Sleep is a dynamic process comprising multiple stages, each associated with distinct electrophysiological properties and potentially serving different functions. While these phenomena are well described in vertebrates, it unclear if invertebrates have sleep stages. We perform local field potential (LFP) recordings on flies spontaneously sleeping, compare their brain activity to induced using either genetic activation of sleep-promoting circuitry or the GABAA agonist Gaboxadol. find transitional stage 7-10 Hz oscillation central during spontaneous sleep. Oscillatory also evident when we acutely activate neurons dorsal fan-shaped body (dFB) Drosophila. In contrast, following Gaboxadol exposure characterized by low-amplitude LFPs, which dFB-induced effects suppressed. thus appears involve at least two stages: increased oscillatory activity, particularly induction, followed desynchronized decreased activity.

Language: Английский

Citations

125