Vision and Locomotion Shape the Interactions between Neuron Types in Mouse Visual Cortex DOI Creative Commons
Mario Dipoppa, Adam Ranson, Michael Krumin

et al.

Neuron, Journal Year: 2018, Volume and Issue: 98(3), P. 602 - 615.e8

Published: April 12, 2018

Language: Английский

Adult mouse cortical cell taxonomy revealed by single cell transcriptomics DOI
Bosiljka Tasic, Vilas Menon, Thuc Nghi Nguyen

et al.

Nature Neuroscience, Journal Year: 2016, Volume and Issue: 19(2), P. 335 - 346

Published: Jan. 4, 2016

Language: Английский

Citations

1761
Targeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization DOI Creative Commons
Elphège P. Nora, Anton Goloborodko, Anne-Laure Valton

et al.

Cell, Journal Year: 2017, Volume and Issue: 169(5), P. 930 - 944.e22

Published: May 1, 2017

Language: Английский

Citations

1616
Diversity of astrocyte functions and phenotypes in neural circuits DOI
Baljit S. Khakh, Michael V. Sofroniew

Nature Neuroscience, Journal Year: 2015, Volume and Issue: 18(7), P. 942 - 952

Published: June 25, 2015

Language: Английский

Citations

1056
A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality DOI Creative Commons
Tanya L. Daigle,

Linda Madisen,

Travis A Hage

et al.

Cell, Journal Year: 2018, Volume and Issue: 174(2), P. 465 - 480.e22

Published: July 1, 2018

Language: Английский

Citations

781
A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging DOI Creative Commons
Nicholas Sofroniew, Daniel Flickinger, Jonathan King

et al.

eLife, Journal Year: 2016, Volume and Issue: 5

Published: June 14, 2016

Imaging is used to map activity across populations of neurons. Microscopes with cellular resolution have small (<1 millimeter) fields view and cannot simultaneously image distributed multiple brain areas. Typical large field microscopes do not resolve single cells, especially in the axial dimension. We developed a 2-photon random access mesoscope (2p-RAM) that allows high-resolution imaging anywhere within volume spanning areas (∅ 5 mm x 1 cylinder). 2p-RAM near diffraction limited (lateral, 0.66 μm, 4.09 μm at center; excitation wavelength = 970 nm; numerical aperture 0.6) over range wavelengths. A fast three-dimensional scanning system efficient sampling neural arbitrary regions interest entire volume. illustrate use by multiple, non-contiguous transgenic mice expressing protein calcium sensors.

Language: Английский

Citations

644
Single-nucleus and single-cell transcriptomes compared in matched cortical cell types DOI Creative Commons
Trygve E. Bakken, Rebecca D. Hodge, Jeremy A. Miller

et al.

PLoS ONE, Journal Year: 2018, Volume and Issue: 13(12), P. e0209648 - e0209648

Published: Dec. 26, 2018

Transcriptomic profiling of complex tissues by single-nucleus RNA-sequencing (snRNA-seq) affords some advantages over single-cell (scRNA-seq). snRNA-seq provides less biased cellular coverage, does not appear to suffer cell isolation-based transcriptional artifacts, and can be applied archived frozen specimens. We used well-matched scRNA-seq datasets from mouse visual cortex compare type detection. Although more transcripts are detected in individual whole cells (~11,000 genes) than nuclei (~7,000 genes), we demonstrate that closely related neuronal types similarly discriminated with both methods if intronic sequences included analysis. estimate the nuclear proportion total mRNA varies 20% 50% for large small pyramidal neurons, respectively. Together, these results illustrate high information content RNA characterization diversity brain tissues.

Language: Английский

Citations

507
Ca2+ signaling in astrocytes from Ip3r2−/− mice in brain slices and during startle responses in vivo DOI
Rahul Srinivasan, Ben Huang, Sharmila Venugopal

et al.

Nature Neuroscience, Journal Year: 2015, Volume and Issue: 18(5), P. 708 - 717

Published: April 20, 2015

Language: Английский

Citations

483
Cerebellar granule cells encode the expectation of reward DOI
Mark J. Wagner, Tony Hyun Kim, Joan Savall

et al.

Nature, Journal Year: 2017, Volume and Issue: 544(7648), P. 96 - 100

Published: March 16, 2017

Language: Английский

Citations

477
Genetic Dissection of Neural Circuits: A Decade of Progress DOI Creative Commons
Liqun Luo, Edward M. Callaway, Karel Svoboda

et al.

Neuron, Journal Year: 2018, Volume and Issue: 98(2), P. 256 - 281

Published: April 1, 2018

Tremendous progress has been made since Neuron published our Primer on genetic dissection of neural circuits 10 years ago. Since then, cell-type-specific anatomical, neurophysiological, and perturbation studies have carried out in a multitude invertebrate vertebrate organisms, linking neurons to behavioral functions. New methods allow systematic classification cell types provide access diverse neuronal for connectivity coding during behavior. Here we evaluate key advances over the past decade discuss future directions.

Language: Английский

Citations

449
Integrated Morphoelectric and Transcriptomic Classification of Cortical GABAergic Cells DOI Creative Commons
Nathan W. Gouwens, Staci A. Sorensen, Fahimeh Baftizadeh

et al.

Cell, Journal Year: 2020, Volume and Issue: 183(4), P. 935 - 953.e19

Published: Nov. 1, 2020

Language: Английский

Citations

447