A Circuit for Integration of Head- and Visual-Motion Signals in Layer 6 of Mouse Primary Visual Cortex DOI Creative Commons
Mateo Vélez‐Fort, Edward F. Bracey, Sepiedeh Keshavarzi

et al.

Neuron, Journal Year: 2018, Volume and Issue: 98(1), P. 179 - 191.e6

Published: March 18, 2018

To interpret visual-motion events, the underlying computation must involve internal reference to motion status of observer's head. We show here that layer 6 (L6) principal neurons in mouse primary visual cortex (V1) receive a diffuse, vestibular-mediated synaptic input signals angular velocity horizontal rotation. Behavioral and theoretical experiments indicate these inputs, distributed over network 100 L6 neurons, provide both reliable estimate and, therefore, physiological separation head-velocity signals. During head rotation presence stimuli, exhibit postsynaptic responses approximate arithmetic sum vestibular response. Functional mapping reveals arrive into via direct projection from retrosplenial cortex. therefore propose processing V1 is multisensory contextually dependent on animal's

Language: Английский

A Designer AAV Variant Permits Efficient Retrograde Access to Projection Neurons DOI Creative Commons

D. Gowanlock R. Tervo,

Bum-Yeol Hwang,

Sarada Viswanathan

et al.

Neuron, Journal Year: 2016, Volume and Issue: 92(2), P. 372 - 382

Published: Oct. 1, 2016

Language: Английский

Citations

1211
Integration of optogenetics with complementary methodologies in systems neuroscience DOI
Christina K. Kim, Avishek Adhikari, Karl Deisseroth

et al.

Nature reviews. Neuroscience, Journal Year: 2017, Volume and Issue: 18(4), P. 222 - 235

Published: March 17, 2017

Language: Английский

Citations

650
A multimodal cell census and atlas of the mammalian primary motor cortex DOI Creative Commons
Edward M. Callaway, Hong‐Wei Dong, Joseph R. Ecker

et al.

Nature, Journal Year: 2021, Volume and Issue: 598(7879), P. 86 - 102

Published: Oct. 6, 2021

Here we report the generation of a multimodal cell census and atlas mammalian primary motor cortex as initial product BRAIN Initiative Cell Census Network (BICCN). This was achieved by coordinated large-scale analyses single-cell transcriptomes, chromatin accessibility, DNA methylomes, spatially resolved morphological electrophysiological properties cellular resolution input-output mapping, integrated through cross-modal computational analysis. Our results advance collective knowledge understanding brain cell-type organization

Language: Английский

Citations

465
Genetic Dissection of Neural Circuits: A Decade of Progress DOI Creative Commons
Liqun Luo, Edward M. Callaway, Karel Svoboda

et al.

Neuron, Journal Year: 2018, Volume and Issue: 98(2), P. 256 - 281

Published: April 1, 2018

Tremendous progress has been made since Neuron published our Primer on genetic dissection of neural circuits 10 years ago. Since then, cell-type-specific anatomical, neurophysiological, and perturbation studies have carried out in a multitude invertebrate vertebrate organisms, linking neurons to behavioral functions. New methods allow systematic classification cell types provide access diverse neuronal for connectivity coding during behavior. Here we evaluate key advances over the past decade discuss future directions.

Language: Английский

Citations

449
A Unified Framework for Dopamine Signals across Timescales DOI Creative Commons
HyungGoo R. Kim, Athar N. Malik, John G. Mikhael

et al.

Cell, Journal Year: 2020, Volume and Issue: 183(6), P. 1600 - 1616.e25

Published: Nov. 27, 2020

Language: Английский

Citations

275
The BRAIN Initiative Cell Census Consortium: Lessons Learned toward Generating a Comprehensive Brain Cell Atlas DOI Creative Commons
Joseph R. Ecker, Daniel H. Geschwind, Arnold R. Kriegstein

et al.

Neuron, Journal Year: 2017, Volume and Issue: 96(3), P. 542 - 557

Published: Nov. 1, 2017

Language: Английский

Citations

272
The gut–brain axis mediates sugar preference DOI
Hwei-Ee Tan, Alexander C. Sisti, Hao Jin

et al.

Nature, Journal Year: 2020, Volume and Issue: 580(7804), P. 511 - 516

Published: April 15, 2020

Language: Английский

Citations

245
Heterogeneity within classical cell types is the rule: lessons from hippocampal pyramidal neurons DOI
Mark S. Cembrowski, Nelson Spruston

Nature reviews. Neuroscience, Journal Year: 2019, Volume and Issue: 20(4), P. 193 - 204

Published: Feb. 18, 2019

Language: Английский

Citations

237
Improved Monosynaptic Neural Circuit Tracing Using Engineered Rabies Virus Glycoproteins DOI Creative Commons
Euiseok J. Kim, Matthew W. Jacobs, Tony Ito-Cole

et al.

Cell Reports, Journal Year: 2016, Volume and Issue: 15(4), P. 692 - 699

Published: April 1, 2016

Monosynaptic rabies virus tracing is a unique and powerful tool used to identify neurons making direct presynaptic connections onto of interest across the entire nervous system. Current methods utilize complementation glycoprotein gene-deleted SAD B19 strain with its glycoprotein, B19G, mediate retrograde transsynaptic spread single synaptic step. In most conditions, this method labels only fraction input would thus benefit from improved efficiency spread. Here, we report newly engineered variants improve efficiency. Among them, oG (optimized glycoprotein) codon-optimized version chimeric consisting transmembrane/cytoplasmic domain B19G extracellular Pasteur glycoprotein. We demonstrate that increases for long-distance up 20-fold compared B19G. oG-mediated will therefore allow identification study more complete monosynaptic neural networks.

Language: Английский

Citations

234
Distributed and Mixed Information in Monosynaptic Inputs to Dopamine Neurons DOI Creative Commons
Ju Tian,

Ryan Huang,

Jeremiah Y. Cohen

et al.

Neuron, Journal Year: 2016, Volume and Issue: 91(6), P. 1374 - 1389

Published: Sept. 1, 2016

Language: Английский

Citations

229